Pathologic and immunohistochemical findings in an outbreak of systemic toxoplasmosis in a mob of red kangaroos     

Mariano Carossino  Rudy Bauer  Mark A. Mitchell  Charles O. Cummings  Anke C. Sthr  Nobuko Wakamatsu  Kimberly Harper  Ingeborg M. Langohr  Kendra Schultz  Maria S. Mitchell  Daniel K. Howe  Udeni B. R. Balasuriya 《Journal of veterinary diagnostic investigation》2021,33(3):554

Toxoplasma gondii is a zoonotic protozoan pathogen that infects many endothermic vertebrates, including humans; the domestic cat and other felids serve as the definitive host. Macropodids are considered highly susceptible to toxoplasmosis. Here, we describe the clinical, pathologic, and immunohistochemical findings of an outbreak of systemic toxoplasmosis in a mob of 11 red kangaroos (Macropus rufus), with high morbidity (73%) and mortality (100%) rates. Affected animals had either severe and rapidly deteriorating clinical conditions or sudden death, which was correlated with widespread necrotizing lesions in multiple organs and intralesional T. gondii organisms identified via MIC3-specific immunohistochemistry and confirmed by REP529-specific rtPCR. Quantification of parasites demonstrated the highest parasite density in pulmonary parenchyma compared with other tissues. Our study highlights the continued importance of this severe condition in Australian marsupials.  相似文献   

Detection of Newcastle disease virus RNA by reverse transcription-polymerase chain reaction using formalin-fixed, paraffin-embedded tissue and comparison with immunohistochemistry and in situ hybridization.   总被引：2，自引：0，他引：2  

Nobuko Wakamatsu  Daniel J King  Bruce S Seal  Corrie C Brown 《Journal of veterinary diagnostic investigation》2007,19(4):396-400

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The duality of teleost gonadotropins   总被引：5，自引：0，他引：5  

Hiroshi Kawauchi  Kunimasa Suzuki  Hiromichi Itoh  Penny Swanson  Nobuko Naito  Yoshitaka Nagahama  Masumi Nozaki  Yasumitu Nakai  Seiga Itoh 《Fish physiology and biochemistry》1989,7(1-6):29-38

The duality of salmon gonadotropins has been proved by biochemical, biological, and immunological characterization of two chemically distinc gonadotropins. GTH I and GTH II were equipotent in stimulating estradiol production, whereas GTH II appears to be more potent in stimulating maturational steroid synthesis. The ratio of plasma levels and pituitary contents of GTHs and the secretory control by a GnRH suggest that GTH I is the predominant GTH during vitellogenesis and early stages of spermatogenesis in salmonids, whereas GTH II is predominant at the time of spermiation and ovulation. GTH I and GTH II are found in distinctly separate cells. In trout, GTH I is expressed first in ontogeny, whereas GTH II cells appear coincident with the onset of spermatogenesis and vitellogenesis, and increase dramatically at the time of final reproductive maturation. Comparison of the amino acid sequences of polypeptides and the base sequences of cDNA revealed that salmon GTH I β is more similar to bovine FSHβ than bovine LHβ and salmon GTH II β shows higher homology to bovine LHβ than to bovine FSHβ. The existence of two pituitary gonadotropins in teleosts as well as tetrapods suggests that the divergence of the GTH gene took place earlier than the time of divergence of teleosts from the main line of evolution leading to tetrapods.  相似文献   

Validation of a food-frequency questionnaire for cohort studies in rural Japan     

Ogawa K  Tsubono Y  Nishino Y  Watanabe Y  Ohkubo T  Watanabe T  Nakatsuka H  Takahashi N  Kawamura M  Tsuji I  Hisamichi S 《Public health nutrition》2003,6(2):147-157

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Mucopolysaccharides in plasma and urine of fattening cattle affected with laminitis     

Y Motoi  Y Kimura  H Wakamatsu  K Shimbayashi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(4):565-569

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The effect of pretreatment with different doses of GnRH to synchronize follicular wave on superstimulation of follicular growth in dairy cattle   总被引：1，自引：0，他引：1  

Sato T  Nakada K  Uchiyama Y  Kimura Y  Fujiwara N  Sato Y  Umeda M  Furukawa T 《The Journal of reproduction and development》2005,51(5):573-578

The objective of this study was to evaluate the efficiency of gonadotropin releasing hormone (GnRH) and GnRH doses in synchronizing follicular wave emergence as a pretreatment for superovulation in cattle. Fourteen Holstein-Friesian cows 6 days from estrus were randomly assigned to receive 100 microg (n=4), 50 microg (n=5), or 25 microg (n=5) of GnRH. Superovulation was induced with injections of porcine FSH (pFSH) twice daily, decreasing the dose (total 42 AU) over 5 days beginning 2.5 days after receiving GnRH. On the 7th and 8th injections of pFSH, 750 microg of PGF(2alpha) was also given. With the exception of one cow that was given 50 microg of GnRH, ovulation was induced in all cows from the three groups and the new follicular wave emergence was observed. The total number of follicles for the 25 microg GnRH group was less than that observed for the 100 microg GnRH group (P<0.05), although there were no differences between the 100 microg, 50 microg and 25 microg GnRH groups with respect to the number of preovulatory follicles (>or=10 mm) and CL. The numbers of normal embryos were greater for the 25 microg GnRH group than the 100 or 50 microg GnRH groups (P<0.01); however, the numbers of ova/embryos did not differ significantly between the three groups. These results suggest that 25 microg of GnRH was sufficient to induce ovulation and follicular wave emergence. On day 6 of the estrous cycle, a reduction of the dose of GnRH to synchronize follicular wave emergence as a pretreatment for superstimulation promotes transferable embryos.  相似文献   

The effect on pathogenesis of Newcastle disease virus LaSota strain from a mutation of the fusion cleavage site to a virulent sequence     

Wakamatsu N  King DJ  Seal BS  Peeters BP  Brown CC 《Avian diseases》2006,50(4):483-488

The principal molecular determinant of virulence of Newcastle disease virus (NDV) is the amino acid sequence at the fusion cleavage activation site. To extend the understanding of the role of the fusion cleavage activation site in NDV virulence, the pathogenesis in chickens of a lentogenic LaSota isolate and two infectious clones, NDFL and NDFLtag, were compared. NDFL is an infectious clone of a lentogenic NDV strain (LaSota E13-1), and NDFLtag is the infectious clone with the fusion cleavage site sequence mutated to the virulent motif. NDFL and NDFLtag were described by Peeters et al. The viruses were inoculated intraconjunctivally into groups of 4-wk-old white leghorn chickens and compared in a pathogenesis study for determination of disease causation (clinical signs of disease, gross lesions, histology, virus isolation, and serology) and viral distribution (presence of viral nucleoprotein and mRNA was detected by immunohistochemistry and in situ hybridization, respectively). The modification of the fusion cleavage activation site to the virulent motif in the infectious clone only slightly increased disease severity and viral distribution in the pathogenesis assessment, even though dramatically increased pathogenicity of NDFLtag was confirmed by standard pathogenicity index tests. The result, that the mutated fusion cleavage site of NDV-NDFLtag had only a small influence on pathogenesis in chickens compared to either E13-1 or NDFL, suggests that the pathogenic effects of NDV are not dependent on the fusion cleavage site alone.  相似文献   

Pathology in practice. Subcutaneous granulomas     

S Gumber  R Schnellbacher  MS Orandle  N Wakamatsu 《Journal of the American Veterinary Medical Association》2012,241(3):327-329

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Reputational damage and the Fukushima disaster: an analysis of seafood in Japan     

Hiroki Wakamatsu  Tsutom Miyata 《Fisheries Science》2017,83(6):1049-1057

As the levels of radioactivity in seafood have fallen back into the safe range, Fukushima fisheries are considering reopening. However, even if seafood from the Fukushima area were sufficiently safe to distribute to seafood markets, its value may be undermined because of the damage done to its reputation by the Fukushima disaster. We quantified consumers’ preferences for seafood from Fukushima and adjacent prefectures to examine the extent of the reputational damage to Fukushima seafood. We conducted a choice experiment to measure consumers’ willingness to pay for seafood from the Fukushima area. We also measured the impact of displaying ecolabels [Marine Stewardship Council (MSC) and Marine Eco-label Japan (MEL)] on Fukushima products. The results indicated that Fukushima products are considerably discounted compared with products displayed as domestic; even products from adjacent prefectures are substantially discounted. By contrast, consumers positively evaluated locally labeled products. We also found that demersal fish are discounted more than pelagic fish that inhabit the ocean surface off the shore of Fukushima.  相似文献   

New method for the estimation of nitrous oxide emission rates from an agricultural watershed     

Nobuko KATAYANAGI  Takuji SAWAMOTO  Atsushi HAYAKAWA  Ryusuke HATANO 《Soil Science and Plant Nutrition》2009,55(4):590-598

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