Challenges facing arthropod biological control: identifying traits for genetic improvement of predators in protected crops     

Pablo Bielza  Virginia Balanza  Dina Cifuentes  Jos E Mendoza 《Pest management science》2020,76(11):3517-3526

Biological control is an efficient pest control method but there are still limitations that are hindering its wider adoption. Genetic improvement of biological control agents (BCAs) can help to overcome these constraints, but the choice of key attributes for better performance that need to be selected is still an open question. Several characteristics have been suggested but the harsh reality is that selective breeding of BCAs has received a lot of attention but resulted in very little progress. Identifying the appropriate traits to be prioritized may be the first step to reverse this situation. In our opinion, the best way is to look at the factors limiting the performance of key BCAs, especially generalist predators (pesticide compatibility, prey‐density dependence, non‐suitable crops, and extreme environmental conditions), and according to these challenges, to choose the attributes that would allow BCAs to overcome those limitations. The benefits of selection for higher resistance to toxins, whether artificially applied (pesticides) or plant produced (plant defenses); increased fitness when feeding on non‐prey food (supplemented or plant‐derived); and better adaptation to extreme temperature and humidity are discussed. In conclusion, genetic improvement of BCAs can bring about new opportunities to biocontrol industry and users to enhance biocontrol resilience. © 2020 Society of Chemical Industry  相似文献   

Revisiting rice breeding methods – evaluating the use of rapid generation advance (RGA) for routine rice breeding     

Bertrand C. Y. Collard  Joseph C. Beredo  Bert Lenaerts  Rhulyx Mendoza  Ronald Santelices  Vitaliano Lopena 《Plant Production Science》2017,20(4):337-352

Rice production needs to increase in the future in order to meet increasing demands. The development of new improved and higher yielding varieties more quickly will be needed to meet this demand. However, most rice breeding programmes in the world have not changed in several decades. In this article, we revisit the evidence in favour of using rapid generation advance (RGA) as a routine breeding method. We describe preliminary activities at the International Rice Research Institute (IRRI) to re-establish RGA on a large scale as the main breeding method for irrigated rice breeding. We also describe experiences from the early adoption at the Bangladesh Rice Research Institute. Evaluation of RGA breeding lines at IRRI for yield, flowering time and plant height indicated transgressive segregation for all traits. Some RGA lines were also higher yielding than the check varieties. The cost advantages of using RGA compared to the pedigree method were also empirically determined by performing an economic analysis. This indicated that RGA is several times more cost effective and advantages will be realized after 1 year even if facilities need to be built. Based on our experience, and previous independent research empirically testing the RGA method in rice, we recommend that this method should be implemented for routine rice breeding in order to improve breeding efficiency.  相似文献   

Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity     

Philippe Régnier  Jorge Bastias  Violeta Rodriguez-Ruiz  Noelia Caballero-Casero  Carmen Caballo  Dolores Sicilia  Axelle Fuentes  Murielle Maire  Michel Crepin  Didier Letourneur  Virginie Gueguen  Soledad Rubio  Graciela Pavon-Djavid 《Marine drugs》2015,13(5):2857-2874

Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of intracellular oxidative stress. The aim of the study was to compare the antioxidant activity of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent or supercritical extraction methods. UV, HPLC-DAD and (HPLC-(atmospheric pressure chemical ionization (APCI)+)/ion trap-MS) characterizations of both natural extracts showed similar compositions of carotenoids, but different percentages in free astaxanthin and its ester derivatives. The Trolox equivalent antioxidant capacity (TEAC) assay showed that natural extracts containing esters displayed stronger antioxidant activities than free astaxanthin. Their antioxidant capacities to inhibit intracellular oxidative stress were then evaluated on HUVEC cells. The intracellular antioxidant activity in natural extracts was approximately 90-times higher than synthetic astaxanthin (5 µM). No modification, neither in the morphology nor in the viability, of vascular human cells was observed by in vitro biocompatibility study up to 10 µM astaxanthin concentrations. Therefore, these results revealed the therapeutic potential of the natural extracts in vascular human cell protection against oxidative stress without toxicity, which could be exploited in prevention and/or treatment of cardiovascular diseases.  相似文献   

Piquerol a and diacetyl piquerol act as energy transfer inhibitors of photosynthesis     

Jos Luis Mendoza  Manuel Jimnez  Blas Lotina-Hennsen 《Pest management science》1994,40(1):37-40

ATP synthesis and phosphorylating (coupled) electron flow of freshly lysed pea chloroplasts were inhibited by piquerol A and diacetyl piquerol, the latter being more active. H + -uptake, basal and uncoupled electron transport were not affected by either compound, indicating that they act as inhibitors of photophosphorylation at the H + -ATPase complex.  相似文献   

Genital lesions in an outbreak of caprine contagious agalactia caused by Mycoplasma agalactiae and Mycoplasma putrefaciens     

Gil MC  Peña FJ  Hermoso De Mendoza J  Gomez L 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(10):484-487

This paper reports on the genital lesions observed in adult male and female goats from a commercial flock in the Extremadura region of southwestern Spain, following an outbreak of contagious agalactia syndrome caused by Mycoplasma agalactiae and M. putrefaciens. Although both species were isolated from several organs, M. putrefaciens was the only agent isolated from the genital lesions reported here, characterized by desquamative salpingitis and cystic catarrhal metritis in females and by testicular degeneration in males. Mycoplasma putrefaciens was isolated from the testes of only one of the males examined.  相似文献   

Disseminated pythiosis in three horses     

Reis JL  de Carvalho EC  Nogueira RH  Lemos LS  Mendoza L 《Veterinary microbiology》2003,96(3):289-295

Three cases of equine subcutaneous pythiosis with dissemination to the internal organs were investigated. The subcutaneous lesions were observed on the mammary gland, nostrils and limbs of the infected horses. Histopathological analysis of the infected tissues revealed a strong eosinophilic reaction, with macrophages, mast cells and giant cells. Sparsely septated hyphal filaments of 4-6 microm diameter were identified in the center of the eosinophilic areas. Specific fluorescent antibody against Pythium insidiosum confirmed the hyphae in the infected tissues in three examined horses. In one of the three cases, the DNA sequences amplified from the infected subcutaneous tissues and internal organs, revealed that P. insidiosum's 18S SSU rDNA amplicons shared 100% identity with those sequences deposit in GenBank. This is the first report confirming by immunochemical and genetic techniques that P. insidiosum can disseminated from superficial to deep structures.  相似文献   

Evaluation of a new immunocapture test for the diagnosis of ovine brucellosis caused by Brucella melitensis   总被引：3，自引：0，他引：3  

Durán-Ferrer M  Mendoza J  Osuna A  Caporale V  Lucas A  León L  Garrido F 《The Veterinary record》2002,151(21):629-635

A new immunocapture technique has been applied to the diagnosis of ovine brucellosis under experimental conditions. The tests were made on a serum bank derived from both young and adult ewes vaccinated conjunctivally with the Rev 1 strain at a dose of 10(8) to 10(9) colony-forming units. Adult ewes were infected experimentally two-and-a-half years after they had been vaccinated and the results were compared with an unvaccinated control group. The condition of each animal in terms of infection with Brucella melitensis was determined by clinical and bacteriological investigations. The development of the immune response was compared by the rose bengal test, the complement fixation test, the Coombs' test and the immunocapture technique for 180 days after the vaccination and for 410 days after the experimental infection, that is, the two following gestations. The results suggest that the new technique is more specific in animals vaccinated conjunctivally, regardless of their age when they were vaccinated. After the experimental infection, significantly (P < 0.05) fewer of the vaccinated sheep which were free of clinical signs and were not excreting B melitensis reacted positively to the test.  相似文献   

Selection for ovulation rate in rabbits     

Noelia Ibez  María Antonía Santacreu  Maria Martinez  Agueda Climent  Agustín Blasco 《Livestock Science》2006,101(1-3):126-133

An experiment of selection for ovulation rate was carried out. Animals were derived from a synthetic line first selected 12 generations for litter size, then 10 generations for uterine capacity. Selection was relaxed for 6 generations. Selection was based on the phenotypic value of ovulation rate with a selection pressure on does of 30%. Males were selected from litters of does with the highest ovulation rate. Males were selected within sire families in order to reduce inbreeding. Ovulation rate was measured in the second gestation by a laparoscopy, 12 days after mating. Each generation had about 80 females and 20 males. Results of three generations of selection were analyzed using Bayesian methods. Marginal posterior distributions of all unknowns were estimated by Gibbs sampling. Heritabilities of ovulation rate (OR), number of implanted embryos (IE), litter size (LS), embryo survival (ES), fetal survival (FS), and prenatal survival (PS) were 0.44, 0.32, 0.11, 0.26, 0.35, and 0.14, respectively. Genetic correlation between OR and LS was 0.56, indicating that selection for ovulation rate can augment litter size. Response to selection for OR was 1.80 ova. Correlated responses in IE and LS were 1.44 and 0.49, respectively. Selection for ovulation rate may be an alternative to improve litter size.  相似文献   

A mathematical model for generating land-use allocation alternatives for agroforestry systems   总被引：2，自引：0，他引：2  

Guillermo A. Mendoza 《Agroforestry Systems》1987,5(4):443-453

Agroforestry has been considered a viable land-use system particularly in the tropical regions. In developing or designing agroforestry systems, various concerns have to be addressed namely: (1) economic; (2) biological and physical; and (3) silvicultural and ecological considerations. This paper addresses these concerns from an interdisciplinary perspective. A land-use allocation model which ensures the simultaneous consideration of these concerns is proposed.  相似文献   

Antibacterial activity of fresh flower heads of Chrysantemum coronarium     

Urzùa A  Mendoza L 《Fitoterapia》2003,74(6):606-608

The antibacterial activity of the methylene chloride and methanol extracts of Chrysantemum coronarium fresh flower heads was investigated. The methylene chloride extract showed discrete antimicrobial activity.  相似文献