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Nancy N. Nichols Nongnuch SutivisedsakBruce S. Dien Atanu BiswasWilliam C. Lesch Michael A. Cotta 《Industrial Crops and Products》2011,33(3):644-647
Dry common beans (Phaseolus vulgaris L.) were evaluated for potential conversion of starch to ethanol. Eight varieties of beans with average starch content of 46% (db) were assayed in a laboratory-scaled process based upon the commercial corn dry grind fermentation process. Ethanol yield was 0.43-0.51 g ethanol/g glucose (0.19-0.23 g ethanol/g beans). The average ethanol yield for the eight bean types was 92% of maximum theoretical yield, demonstrating that starch from beans could be efficiently converted to ethanol. Ethanol concentration obtained from 20% (w/w) solids loading was 3.5-4.4% (w/v). The residual fermentation solids contained, on a dry basis, 37.1-43.6% crude protein, 10.8-15.1% acid detergent fiber and 19.1-31.3% neutral detergent fiber. 相似文献
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JF de Brito Galvao WC Kisseberth S Murahari S Sutayatram DJ Chew N Inpanbutr 《American journal of veterinary research》2012,73(8):1262-1272
Objective-To evaluate in vitro effects of gemcitabine alone and in combination with carboplatin on canine transitional cell carcinoma (TCC) cell lines. Sample-In vitro cultures of 5 canine TCC cell lines. Procedures-Cells were treated with gemcitabine, carboplatin, or a combination of both at various concentrations. Cell proliferation was assessed via a fluorescence-based microplate cell proliferation assay. Cell cycle was evaluated via propidium iodide staining, and apoptosis was assessed by measurement of caspase 3 and 7 enzymatic activity. Synergy between gemcitabine and carboplatin was quantified via combination index analyses. Results-Treatment of 5 canine TCC cell lines with gemcitabine or carboplatin decreased cell proliferation, increased apoptosis, and induced cell cycle arrest. Cell cycle arrest and apoptosis were markedly increased when cell lines were treated with both gemcitabine and carboplatin simultaneously or sequentially. Order of administration during sequential treatment did not consistently affect cell proliferation results in TCC cell lines. When TCC cell lines were treated with gemcitabine and carboplatin in combination at therapeutically relevant concentrations (gemcitabine concentration, < 10μM; carboplatin concentration, < 250μM), a significant decrease in cell proliferation was observed, compared with cell proliferation following treatment with gemcitabine or carboplatin alone. In combination, the effects of gemcitabine and carboplatin were synergistic in 3 of 5 cell lines and additive in the other 2. Conclusions and Clinical Relevance-Gemcitabine had antitumor effects on canine TCC cells in vitro, and the combination of gemcitabine and carboplatin had synergistic activity at biologically achievable concentrations. 相似文献
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Megan E. Pozza Thattawan Kaewsakhorn Chumnan Trinarong Nongnuch Inpanbutr Ramiro E. Toribio 《Veterinary journal (London, England : 1997)》2014,199(3):451-456
Vitamin D is essential in calcium and phosphorus regulation, bone physiology, cell proliferation and epithelial integrity. Literature on vitamin D in growing horses is sparse, and the effect of age on vitamin D has not been evaluated in equids in the United States or in tropical countries. The goal of this study was to determine if there was an effect of age on serum 25(OH)D3 concentrations in equids in the US (Ohio/Kentucky) and Thailand (Chiang Rai and Kanchanaburi) during the same time of the year. Blood samples were collected from healthy ponies (n = 21) and Thoroughbred foals (n = 13), yearlings (n = 10), and horses (n = 20) in Thailand and from Thoroughbred foals (n = 10) and horses (n = 17) in the US. Serum concentrations of 25(OH)D3, calcium and phosphorus were measured.In both countries, serum 25(OH)D3 concentrations were lower in foals than in yearlings and adult horses. Serum 25(OH)D3 concentrations were higher in horses than in ponies in Thailand, but were not different between horses from either country. Calcium concentrations were not different between groups or location. In both countries, phosphorus concentrations were higher in foals than in older groups; however, were not different between ponies and horses. This study shows that independent of geography there are age-related differences in 25(OH)D3 concentrations in horses and further confirms that 25(OH)D3 concentrations are lower in horses compared to other species. The information will serve as the basis for future clinical studies and to help understand better the pathophysiology of equine disorders associated with calcium and phosphorus dysregulation. 相似文献
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Tawin Inpankaew Tawisa Jiyipong Kannika Wongpanit Nongnuch Pinyopanuwat Wissanuwat Chimnoi Chanya Kengradomkij Xuenan Xuan Ikuo Igarashi Lihua Xiao Sathaporn Jittapalapong 《Tropical animal health and production》2014,46(2):487-490
The objectives of this study were to determine the individual and herd-level prevalence and genotype of Cryptosporidium and to identify putative risk factors associated with Cryptosporidium spp. infections in water buffaloes in northeast Thailand. Fecal samples from 600 water buffaloes of 287 farms in six provinces were collected and tested using DMSO-modified acid-fast staining and polymerase chain reaction. The overall prevalence of Cryptosporidium infections in buffaloes was 5.7 and 8.7 % among individual animals and herds, respectively. The provinces with highest infected Cryptosporidium were located in the Sakon Nakhon Basin in the northern part of the region. In addition, higher herd prevalence was observed among farms with more than five buffaloes (30 %) than those with five or less animals (16.2 %). Thirty (88.2 %) of the 34 Cryptosporidium-positive samples were Cryptosporidium parvum and four (11.8 %) were Cryptosporidium ryanae. 相似文献
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1,25‐Dihydroxyvitamin D3 and its analogues increase catalase at the mRNA,protein and activity level in a canine transitional carcinoma cell line 
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下载免费PDF全文 R. P. Middleton R. Nelson Q. Li A. Blanton J. A. Labuda J. Vitt N. Inpanbutr 《Veterinary and comparative oncology》2015,13(4):452-463
Antioxidant enzymes, such as catalase, superoxide dismutases (SOD), MnSOD and Cu/ZnSOD, protect cells by scavenging reactive oxygen species (ROS). Numerous studies have reported the anti‐cancer effects of 1,25‐dihydroxyvitamin D3 (calcitriol) and its related analogues, seocalcitol and analogue V. In this study, canine bladder transitional cell carcinoma (cbTCC) cells were used to determine effects of calcitriol and its related analogues on antioxidant enzyme gene expression, protein expression and activity. Catalase mRNA was increased in response to calcitriol (10?7 M), and seocalcitol (10?7 and 10?9 M). MnSOD mRNA was decreased in response to calcitriol at 10?7 M. Catalase was significantly increased in response to calcitriol (10?7 and 10?9 M), and seocalcitol (10?9 M). Catalase enzymatic activity increased in response to calcitriol, seocalcitol and analogue V (10?9 M). In addition, global gene expression analysis identified the involvement of mitogen‐activated protein kinase (MAPK) signalling in cbTCC's response to calcitriol and seocalcitol treatment. 相似文献
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Jittapalapong S Nimsupan B Pinyopanuwat N Chimnoi W Kabeya H Maruyama S 《Veterinary parasitology》2007,145(1-2):138-141
Cats and dogs are the most popular pet animals worldwide. Cats are the natural reservoir of Toxoplasma gondii and excrete the resistant oocyst to environments. On the other hands, dogs play a role in the mechanical transmission of the parasite. Stray cats and dogs in the Bangkok metropolitan area are becoming a public concern because there is a considerable increase in their number annually. These facts indicate the risk of mechanically spreading zoonoses including toxoplasmosis to humans since human acquire the infection from infected mammals, either directly or indirectly. In the present study, the presence of T. gondii antibodies was examined in 592 cats and 427 dogs from October 2001 to September 2002 by using a latex agglutination test. T. gondii antibodies were detected in 65 (11.0%) of the 592 cats and 40 (9.4%) of the 427 dogs. The antibody titers in the positive animals ranged from 1:64 to 1:2048. Seroprevalence was significantly higher in female cats than in male cats. The present study suggested that T. gondii was widespread in the stray animals in the Bangkok metropolitan area; therefore, it is essential to control the number of stray cats and dogs in order to reduce the transmission of toxoplasmosis to animals and humans. 相似文献
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Thiangtum K Nimsuphun B Pinyopanuwat N Chimnoi W Tunwattana W Tongthainan D Jittapalapong S Rukkwamsuk T Maruyama S 《Veterinary parasitology》2006,136(3-4):351-355
The prevalence of antibodies against Toxoplasma gondii was investigated by commercial latex agglutination test kit (Toxocheck-MT 'Eiken') in captive felids maintained at zoos and a wildlife breeding center in different geographic regions of Thailand. Sera from a total of 136 captive felids of 12 species was obtained between 2002 and 2004. The overall seroprevalence of T. gondii was found in 21 of 136 (15.4%) felids. The titers varied from 1:64 (eight samples) to 1:8192 (one sample). The seroprevalence in different geographic regions were from 0% in the northern area to 23% in the southern area. This study suggested a widespread exposure of captive felids to T. gondii in Thailand and this is the first report of serologic analysis for T. gondii in captive felids in Southeast Asia. 相似文献
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Jittapalapong S Sangvaranond A Pinyopanuwat N Chimnoi W Khachaeram W Koizumi S Maruyama S 《Veterinary parasitology》2005,127(1):17-22
Goats are important domestic animals in the south of Thailand due to the minimal cost of rearing and maintaining them, and their production of both meat and milk. Toxoplasmosis is one of the most threatening parasitic zoonoses and the causative agent Toxoplasma gondii uses a wide range of warm-blooded intermediate hosts including the goat. The objective of this study was to assess the seroprevalence of antibodies to T. gondii in goats of Satun Province in Thailand. A total of 631 goat sera were examined for antibodies against toxoplasmosis with commercial latex agglutination test kits (Toxocheck-MT 'Eiken'). Of these, 176 (27.9%) were found to be positive to T. gondii; antibody titers ranged from 1:64 to 1:4096 (1:64 cut-off). Female goats were 1.73 times more likely than male to be seropositive (odds ratio [OR]=1.73; 95% confidential interval [CI]=1.11, 2.73). Dairy goats were more likely to be seropositive than meat goats (OR=1.36; 95% CI=0.84, 2.20). Goats were infected with T. gondii with acquisition of age because older goats were more likely to be seropositive than young goats under 1-year-old (for 1-2 years, OR=19.6; 95% CI=0.92, 4.15, for >2 years, OR=2.70; 95% CI=1.26, 5.80). The high seroprevalence of T. gondii antibodies found in the present study suggested widespread exposure of goats in Satun Province to T. gondii. 相似文献
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