全文获取类型
收费全文 | 98篇 |
免费 | 9篇 |
专业分类
林业 | 1篇 |
农学 | 1篇 |
1篇 | |
综合类 | 1篇 |
畜牧兽医 | 100篇 |
植物保护 | 3篇 |
出版年
2023年 | 2篇 |
2020年 | 2篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2011年 | 6篇 |
2010年 | 2篇 |
2009年 | 8篇 |
2008年 | 1篇 |
2006年 | 2篇 |
2004年 | 4篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
排序方式: 共有107条查询结果,搜索用时 31 毫秒
1.
2.
Effect of porcine reproductive and respiratory syndrome virus (PRRSV) on alveolar lung macrophage survival and function 总被引:9,自引:0,他引:9
Porcine reproductive and respiratory syndrome virus (PRRSV) recently emerged as an important cause of reproductive disorders and pneumonia in domestic pigs throughout the world. Acute cytocidal replication of PRRSV in alveolar lung macrophages causes the acute pneumonia; however, it remains largely unresolved whether there may also be a predisposition to longer-term local immunodeficiency in the PRRSV-convalescent lung. We applied various flow cytometric techniques to study the interplay between PRRSV replication and macrophage viability/function in pure cultures of porcine alveolar lung macrophages. Monitored by flow cytometric detection of intracellular PRRSV nucleocapsid protein, acute (24 h post infection) PRRSV replication did not impede the ability of alveolar macrophages to ingest fluorescently labelled Escherichia coli. At 48 h post infection, PRRSV-induced cytotoxicity (quantitated by flow analysis of cell size and membrane integrity) led to 40% reduction in the total number of phagocytozing cells. However, viable/uninfected macrophages in PRRSV-infected cultures exhibited normal phagocytic ability at 48 h, indicating that no soluble phagocytosis-suppressive mediators were induced by PRRSV infection in this system. In short, in our minimal system containing only a single cell type, phagocytosis-suppressive effects of PRRSV infection were detected, that acted at the culture level by reducing the total number of alveolar lung macrophages. 相似文献
3.
Oleksiewicz MB Kristensen B Ladekjaer-Mikkelsen AS Nielsen J 《Veterinary immunology and immunopathology》2002,86(1-2):55-77
The extracellular domains of swine leukocyte antigen class I (SLA-I, major histocompatibility complex protein class I) were cloned and sequenced for two haplotypes (H4 and H7) which do not share any alleles based on serological typing, and which are the most important in Danish farmed pigs. The extracellular domain of SLA-I was connected to porcine beta2 microglobulin by glycine-rich linkers. The engineered single-chain proteins, consisting of fused SLA-I and beta2 microglobulin, were overexpressed as inclusion bodies in Escherichia coli. Also, variants were made of the single-chain proteins, by linking them through glycine-rich linkers to peptides representing T-cell epitopes from classical swine fever virus (CSFV) and foot-and-mouth disease virus (FMDV). An in vitro refold assay was developed, using a monoclonal anti-SLA antibody (PT85A) to gauge refolding. The single best-defined, SLA-I restricted porcine CD8(+) T-cell epitope currently known is a 9-residue peptide from the polyprotein of CSFV (J. Gen. Virol. 76 (1995) 3039). Based on results with the CSFV epitope and two porcine haplotypes (H4 and H7), the in vitro refold assay appeared able to discriminate between peptide-free and peptide-occupied forms of SLA-I. It remains to be seen whether the rapid and technically very simple in vitro refold assay described here will prove generally applicable for the screening of virus-derived peptides for SLA-I binding. 相似文献
4.
C.R.E. Halnan MA Vet MB PhD FRCVS C.F.P. Irwin BVSc MACVSc 《Journal of Equine Veterinary Science》1985,5(6):328-329
A Thoroughbred mare, barren during three years at stud, unresponsive to therapy, and not palpably abnormal anatomically was found to have hypoplasia of the uterine tubes after excision at laparotomy. The cause was an unbalanced genotype. The mosaic karyotype, determined on a blood sample, was indispensible in diagnosis and the case extends the potential for cytogenetic prognostic procedures. 相似文献
5.
JONATHAN L. STOWATER DVM MS CARL A. KIRKER-HEAD MA VET MB MRCVS RICHARD M. JAKOWSKI DVM PHD 《Veterinary radiology & ultrasound》1986,27(4):115-117
Osteochondrosis of the lateral femoral condyles was diagnosed radiographically in an 8-month-old, female Arabian horse, which had been presented with a hindlimb lameness. The diagnosis was confirmed by gross and microscopic pathology. The location of the lesions was considered unusual for osteochondrosis in the horse. 相似文献
6.
7.
8.
Recombinant Bone Morphogenetic Proteins: Novel Substances for Enhancing Bone Healing 总被引:1,自引:0,他引:1
CARL A. KIRKER-HEAD MA Vel MB MRCVS Diplomate ACVS Diplomate ECVS 《Veterinary surgery : VS》1995,24(5):408-419
Bone morphogenetic proteins (BMPs) are differentiative factors whose principal function is to induce transformation of undifferentiated mesenchymal cells into chondroblasts and osteoblasts in a dose-dependent manner. Bone morphogenetic proteins have been isolated postnatally in mammals from bone matrix, periosteal cells, mesenchymal cells of marrow stroma, tooth anlagen, and cells of osteosarcoma and chondrosarcoma. Distribution in additional embryonic tissues implies a broader organogenic function. Bone morphogenetic proteins are the only differentiative factors able to singularly induce de novo bone formation in vitro and in vivo. Recombinant DNA technology allows their production in large and highly purified quantities. The BMPs' osteoinductive ability has been shown with a variety of carriers including collagens and polymers at heterotopic and orthotopic sites in a wide range of species. They are presently being readied for clinical use as alternatives to bone grafts. Other potential applications include use as pulp capping agents, promoters of implant osteointegration and soft tissue reunion with bone, treatments for nonadaptive bone disease, and implants for use with mitotically expanded skeletal stem cell populations. Errors in the genetic coding of BMPs may manifest as clinical disease entities. 相似文献
9.
Malm O Guimarães JRD Castro MB Bastos WR Viana JP Branches FJP Silveira EG Pfeiffer WC 《Water, air, and soil pollution》1997,97(1-2):45-51
Informal economy of gold mining has contaminated some important river basins in Amazon. Follow-up studies on critical compartments showed some areas with high Hg levels in fish as well as in human hair samples. Average Hg in piscivorous fish in the Madeira river itself was 846 ppb (N=284) with a maximum of 3921 ppb. Mercury in fish from non polluted areas in this basin shows high variability, even for single species. A seasonal variation in Hg content was observed, with higher values at the end of the dry season. In the upper Tapajós basin comparable values were found for fish but with a definite decreasing trend downstream. Average value for piscivorous fish in the whole Tapajós basin is 482 ppb (N=122) with a maximum value of 3770 ppb. Hair Hg was higher in fishing villages in the Tapajós (average: 17 ppm; with N=432 and maximum value of 176 ppm) than in the Madeira (average: 9 ppm; N=169; maximum 71 ppm), and data from some areas of the Tapajós suggest a decrease with time. Mercury was much higher in urine of goldshop workers in Santarém (low Tapajós) than in Alta Floresta (high Tapajbs) and show a decreasing trend in both cases, probably related to the significant decline in gold mining activities during the study period (1986–1994). 相似文献
10.
The situation in Thoroughbred breeding in relation to cytogenetics is considered and it is indicated where screening of horses will be useful in practice. The techniques involved are explained and karyotypes are illustrated. The present state of knowledge in horse cytogenetics is reviewed. The types of abnormality are described in relation to phenotype. Future promise is discussed in relation to the human field, concluding that with the refined methods now available a wider rangeof meaningful aberrations will be uncovered. 相似文献