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The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria.  相似文献   
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Population growth, urban expansion and economic development are increasing competition for water use between agriculture and other users. In addition, the high rate of soil degradation and declining soil moisture in the Sub-Saharan African Region have called for several crop production management and irrigation options to improve soil fertility, reduce water use by crops and produce ‘more crops per drop of water’. Notwithstanding this, considerable variations exist in the literature on water-use efficiency, WUEcwu (economic yield per water used) for maize (Zea mays L.) across climates and soil management practices. Different views have been expressed on the effect of different rates of nitrogen (N) application on transpiration efficiency, TE (biomass produced per unit of water transpired). The objectives of the study were to assess the effect of different rates of N-enriched municipal waste co-compost and its derivatives on TE, WUEcwu and yield of maize (Z. mays L.) in comparison to inorganic fertiliser. The greenhouse pot experiment was conducted in Accra, Ghana on a sandy loam soil (Ferric Lixisol) using a split plot design. The main plot treatments were soil (S), dewatered faecal sludge (DFS), municipal solid waste compost (C), co-compost from municipal solid waste and dewatered faecal sludge (Co), compost enriched with (NH4)2SO4 (EC), co-compost enriched with (NH4)2SO4 (ECO), (NH4)2SO4 and NPK15-15-15 + (NH4)2SO4. The sub-plot treatments were different rates of application of nitrogen fertiliser applied at the rate of 91, 150 and 210 kg N ha−1 respectively. Maize cv. Abelehii was grown in a poly bag filled with 15 kg soil. Eight plants per treatment were selected randomly and used for the collection of data on growth parameters forth-nightly. At physiological maturity two plants per treatment were also selected randomly from each treatment plot for yield data. The results showed that TE of maize (Z. mays) varied for the different treatments and these are 6.9 Pa in soil (S) alone to 8.6 Pa in ECO. Increase in N application rate increased TE at the vegetative phase for fast nutrient releasing fertilisers (DFS, ECO, EC, NPK + (NH4)2SO4, (NH4)2SO4) and at the reproductive phase for slow nutrient releasing fertilisers (C and CO). Water-use efficiency increased significantly as rate of N application increased. Treatment ECO improved crop WUEcwu and was 11% and 4 times higher than that for NPK + (NH4)2SO4 or soil alone; and 18-36% higher than those for DFS and CO. Treatment ECO used less amount of water to produce dry matter yield (DMY) and grain yield (GY) that was 5.2% and 12.6%, respectively, higher than NPK + (NH4)2SO4. Similarly, the DMY and GY for ECO was 8.9-18.5% and 23.4-34.7%, respectively, higher than DFS and CO. High nutrient (N and K) uptake, TE, and low leaf senescence accounts for 83% of the variations in DMY whereas WUEcwu accounts for 99% of the variations in GY. Thus, the study concluded that different sources of fertiliser increased TE and WUEcwu of maize differently as N application rate increases.  相似文献   
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In a comparison of potato dextrose agar (PDA) and Czapek’s Dox broth (CDB) agar with and without benomyl, aa growth media forHelminthosporium pedicellatum Henry (Trichometasphaeria pedicellatum Nelson), PDA was 1.5 times more efficient than CDB under all conditions. Growth rate was negatively correlated with benomyl concentration, and was inhibited 11% and 74% at benomyl concentrations of 10 μg and 200 μar, respectively, on both media. Pure isolates of the fungus were recovered from corn roots only where roots had been sterilized with 10% sodium hypochlorite. Root sterilization may thus be more important than medium amendment in isolating pure cultures ofedicellatum from plant roots.  相似文献   
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The study assessed the effects of seed sources and pretreatments on the germination of Dialium guineense Willd. Seeds were collected from four sources in Nigeria: Ijebu-Ode, Iperu, Odogbolu, and Moniya. Six hundred seeds from each progeny were weighed to determine differences in seed weight. Seeds were pretreated using six treatments: control, soaking in water at 90°C and allowing to cool overnight, soaking in water at room temperature, soaking in concentrated sulphuric acid (H2SO4) for 5 min, 10 min, and 15 min represented as T1, T2, T3, T4, T5, and T6, respectively. Thirty seeds were assigned to each treatment with three replicates for all the seed sources studied. Seeds were sown in germination trays containing washed-sterilized river sand and set under high humidity propagator. Germination counts were taken daily until no further germination took place for 7 d. In all the progeny sources studied, T6 gave the highest mean germination percentage of 59.2 followed by T5 (57.5), T4 (35.8), T2 (21.7), T1 (8.3), while T3 gave the lowest value of 6.7. Analysis of variance revealed significant differences at p ≤ .05 in seed weight, mean germination percentage among seed sources, and treatments. Velvet tamarind has inherent seed coat dormancy that can be removed by pretreatment with concentrated H2SO4 for 10 and 15 min.  相似文献   
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