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M Jeseta D Ctvrtlikova Knitlova K Hanzalova P Hulinska S Hanulakova I Milakovic L Nemcova J Kanka M Machatkova 《Reproduction in domestic animals》2014,49(3):469-475
This study was designed to specify chromatin and mitochondrial patterns in bovine oocytes with different meiotic competence in relation to maturation progress, resumption of meiosis, MII onset and completion of maturation. Oocytes with greater or lesser meiotic competence, recovered separately from medium (MF) and small follicles (SF), were categorized according to morphology. Four oocyte categories, healthy and light‐atretic MF and healthy and light‐atretic SF oocytes were matured and collected at 0, 3, 7, 16 and 24 h of maturation. Specific differences in terms of chromatin and mitochondrial patterns were found among the maturing oocyte categories. Resumption of meiosis was accelerated in light‐atretic oocytes, as compared with healthy oocytes, regardless of their meiotic competence. More competent oocytes activated mitochondria twice during maturation, before resumption of meiosis and before completion of maturation, while less competent oocytes did it only once, before completion of maturation. Changes in mitochondrial activity differed in light‐atretic compared with healthy in both more and less competent oocytes. Healthy meiotically more competent oocytes formed clusters and produced ATP for the whole time of maturation until its completion, while light‐atretic more competent oocytes and healthy less competent oocytes reduced these activities earlier, at MII onset. Contrary to these oocyte categories, light‐atretic less competent oocytes increased cluster formation significantly before resumption of meiosis. It can be concluded that bovine oocytes with different meiotic competence and health differed in the kinetics of mitochondrial patterns during maturation. 相似文献
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Ivona Travnickova Pavlina Hulinska Svatava Kubickova Katerina Hanzalova Bartozs Kempisty Lucie Nemcova Marie Machatkova 《Reproduction in domestic animals》2021,56(6):864-871
The study investigated the effects of sperm sorting, capacitation treatment and co-cultivation on sexed bovine in vitro embryo production. The effect of treatment and co-culture on production of embryos of the preferred sex from unsorted sperm was also studied. Sperm from five breeding bulls was used for fertilization of mature oocytes as follows : Experiment 1, sorted and unsorted sperm (bulls A-E) treated only with heparin in standard co-cultures; Experiment 2, sorted sperm (bulls A-E) treated with heparin-PHE (penicillamine, hypotaurine, and epinephrine) or heparin-caffeine in drop co-cultures; and Experiment 3, unsorted sperm (bull E) treated with either heparin-PHE or heparin-caffeine in both standard and drop co-cultures. In all bulls, treatment with heparin resulted in significantly (p < .05) reduced cleavage and blastocyst rates from sorted sperm, as compared with those from unsorted sperm. In bulls A, B, D and E, treatment of sorted sperm with heparin-PHE in drops significantly increased the blastocyst rate (p < .05). In unsorted sperm of bull E, heparin-PHE treatment in drops resulted in the XX/XY sex ratio inverse to that obtained by heparin-caffeine treatment in standard co-cultures (32.3%/67.7% and 66.7%/33.3%, respectively). In conclusion, the treatment of sorted sperm with heparin-PHE in modified drop co-cultures can be recommended for production of in vitro sexed embryos. The use of unsorted sperm for production of embryos of the preferred sex by selected capacitation treatment and co-culture can be the method of choice in bulls with low IVF yields from sorted sperm. 相似文献
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Fruit from Granny Smith, Fyriki, Fuji Kiku 8, and Imperial Double Red Delicious apple (Malus domestica Borkh.) trees planted in single or double rows, were harvested from different positions in the canopy, during the last month before commercial harvest. Fruit physico-chemical and antioxidant capacity, using the radical DPPH, were measured, in skin and flesh tissue. Greater total phenol content and total antioxidant capacity per fresh weight and serving portion of skin and flesh tissue, were found in Imperial Double Red Delicious and Fyriki apples, compared to Granny Smith and Fuji Kiku 8. The variation among cultivars in antioxidant contents was greater in peeled, compared with unpeeled fruit, highlighting the importance of eating unpeeled fruit. During the last two weeks before commercial harvest the total phenol content and total antioxidant capacity per fresh weight increased in skin of Granny Smith (by 24% and 42%, respectively) and Fuji Kiku 8 (by 19% and 27%, respectively). Fruit from the more sun—exposed parts of canopy had usually better red coloration and the effect was more pronounced in Fuji Kiku 8 and Fyriki, followed by Imperial Double Red Delicious and a lower effect was found in Granny Smith. Greater total soluble solid content, but not flesh firmness, were also found in fruit from the more sun—exposed parts of canopy, in all cultivars. The skin of fruit from the upper positions in the canopy had greater total phenol content and total antioxidant capacity, in all cultivars, apart from Fyriki. Antioxidant contents in flesh tissue were also greater in the upper positions of canopy in Fuji Kiku 8 and Imperial Double Red Delicious, and to our knowledge this is the first report on plant canopy effects on apple flesh antioxidant compounds. 相似文献
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A study was conducted to investigate the variability in the fruit antioxidant content and physical characters of six clingstone cultivars and three breeding selections of peach grafted on three rootstocks. The parameters measured were fruit weight, fruit and stone dimensions, flesh color using CIELAB color variables, total antioxidant activity using the radical DPPH, total phenolics, ascorbic acid, soluble solids and total acid content. Fruit from cultivar PI-E45 had the highest total antioxidant activity (10.7 mg g−1 DW) and total phenolic (6.9 mg g−1 DW) content, which were up to 6.3- and 5.3-fold greater, respectively, compared with the rest studied cultivars. The highest ascorbic acid content was found in Fortuna (7.3 mg 100 g−1 FW) and was up to 1.4-fold greater compared with the rest studied cultivars. A high correlation between AEAC and the phenolic content was found, but not between AEAC and the ascorbic acid content. The largest fruit was harvested in cultivar Andross followed with a descending order by PI-E45, PI-IB42, PI-A37 (seedlings of Andross), Fortuna and Loadel ? Everts and Catherina ? Romea. Changes in the fruit weight were usually according to changes in stone width. The fruit and stone shape differed among the cultivars but not among the rootstocks studied. Effects of rootstock on the fruit antioxidant contents were not pronounced. Nevertheless rootstocks altered the fruit weight since in all cultivars, apart from Romea and Catherina, when grafted on GF 677 produced the largest fruit (mean 186 g) followed by PR204 (mean 176 g) and even smaller by KID1 (mean 161 g). Results from correlation analyses showed that flesh brightness (measured in frozen fruit) may suggest for more nutritional flesh and small sized fruit may contain a redder and less bright colored flesh. 相似文献
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Energy Status Characteristics of Porcine Oocytes During In Vitro Maturation is Influenced by Their Meiotic Competence 
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下载免费PDF全文 I Milakovic M Jeseta S Hanulakova D Knitlova K Hanzalova P Hulinska L Machal B Kempisty P Antosik M Machatkova 《Reproduction in domestic animals》2015,50(5):812-819
The characteristics of energy status in porcine oocytes as related to their meiotic competence and in vitro maturation were studied. Cycling pubertal gilts in the early luteal to early follicular phases of the ovarian cycle were used as oocyte donors. The oocytes recovered from medium (MF) or small follicles (SF) were considered meiotically more or less competent, respectively. A half of oocytes from each category was matured by the standard protocol. The oocytes were examined before or after maturation by confocal microscopy, a bioluminescent cell assay and Western blotting. Four experiments, each in triplicate, were performed to assess both SF and MF oocytes in terms of metabolic units formed by mitochondria and lipids, ATP and lipid consumption and lipid droplets with adipose differentiation‐related protein (ADRP) expression. The proportion of oocytes with metabolic units, the mean ATP content and the number of lipid droplets per oocyte, and the relative number of lipid droplets with ADRP expression were significantly higher in the MF compared to SF oocytes before maturation. On the other hand, after maturation, there was an increase in the proportion of oocytes with metabolic units and the relative number of lipid droplets with ADRP expression in the SF compared to MF oocytes. In conclusion, specific differences in energy characteristics between porcine oocytes with different meiotic competence were found. Meiotically more competent oocytes are more advanced in terms of energy reserves before maturation, while meiotically less competent oocytes are more active in replenishing energy stores during maturation. 相似文献
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Faldyna M Sinkora J Knotigova P Rehakova Z Moravkova A Toman M 《Veterinary immunology and immunopathology》2003,95(3-4):165-176
Dogs represent both an important veterinary species and a convenient model for allogeneic hematopoietic stem cell transplantation. Even though anti-canine CD34 antibodies have recently become available, little is known about hematopoietic lineages in dogs, partially because CD34- cells have been ignored in all analyses performed so far. In this study, we have focused on the bone marrow mononuclear compartment to provide an additional piece of information on the phenotype of CD34+ progenitors and to identify the dominant CD34- population. We have shown that, in contrast to the adults, mature lymphocytes are scarce in neonatal dog bone marrow. Using cross-reactive antibodies against CD79alpha we have shown that the B lineage of hematopoiesis strongly prevails. CD34+ cells were shown to be positive for MHC class II and SWC3, a member of the signal regulatory protein family. 相似文献
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Drogoudi PD Vemmos S Pantelidis G Petri E Tzoutzoukou C Karayiannis I 《Journal of agricultural and food chemistry》2008,56(22):10754-10760
Fruit physical and chemical characters of 29 apricot cultivars of Greek and American origin and their hybrids were evaluated using correlation and principal component analysis. A remarkable variation was observed in the total phenol content (0.3-7.4 mg gallic acid equivalent g(-1) FW) and total antioxidant capacity (0.026-1.858 mg ascorbic acid equivalent g(-1) FW), with the American origin cultivars Robada and NJA(2) and the new cultivar Nike exhibiting the greatest values. The cultivar Tomcot and hybrid 467/99 had the highest content of total carotene (37.8 microg beta-carotene equivalent g(-1) FW), which was up to four times greater as compared with the rest of studied genotypes. The dominant sugar in fruit tissue was sucrose, followed second by glucose and third by sorbitol and fructose-inositol. The new cultivars Nike, Niobe, and Neraida contained relatively higher contents of sucrose and total sugars, while Ninfa and P. Tirynthos contained relatively higher contents of K, Ca, and Mg. Correlation analysis suggested that late-harvesting cultivars/hybrids had greater fruit developmental times (r = 0.817) and contained higher sugar (r = 0.704) and less Mg contents (r= -0.742) in fruit tissue. The total antioxidant capacity was better correlated with the total phenol content (r = 0.954) as compared with the total carotenoid content (r = 0.482). Weak correlations were found between the fruit skin color and the antioxidant contents in flesh tissue. Multivariate analysis allowed the grouping of variables, with more important variables being the harvest date, fruit developmental time, skin Chroma, sorbitol, and total sugar, K and Mg contents. Plotting the genotypes in a dendrogram revealed cases of homonymy between parents and hybrids, although independent segregation of the measured traits after hybridization was also found. 相似文献