Prevalence of ovine and bovine respiratory syncytial virus infections in cattle determined with a synthetic peptide-based immunoassay.     

S T Grubbs  S A Kania  L N Potgieter 《Journal of veterinary diagnostic investigation》2001,13(2):128-132

Subgroup-specific peptide-based enzyme-linked immunosorbent assays from the G-protein of the ovine and bovine respiratory syncytial virus (RSV), respectively, were used to determine the prevalence of the ovine and bovine subgroup strains of RSV infections in cattle. A total of 1,102 bovine serum samples were obtained from 6 diagnostic laboratories located in the northwestern and the southeastern USA and were tested for antibody to either the bovine or ovine subgroups of RSV. Antibody to viruses from each subgroup was present in samples from each region and all states tested. The Southeast had a higher prevalence of the bovine subgroup strains (69.5%). Then did the Northwest (40.9%). The prevalence of the ovine strain was similar for the two regions (16.7% in the southeast, 14.9% in the northwest). The overall prevalence was 56.6% for the bovine strain and 15.9% for the ovine strain. These results suggest members of the ovine subgroup of RSV circulate in the cattle population but with less frequency than those viruses of the bovine subgroup.  相似文献   

Evaluation of an electronic device used as a quick screening method to detect changes in the red cell content of the blood in horses participating in endurance trail rides     

A. De Waal  F. J. Potgieter 《Veterinary research communications》1991,15(4):309-314

Dehydration, to varying degrees, will inevitably occur in horses participating in endurance trail rides. This water loss is directly related to the amount of body water lost through evaporative cooling, that in turn being related to the amount and rate of work performed, and to the environmental temperature and humidity.An electronic apparatus that can substitute for the conventional and time-consuming venous haematocrit was developed to measure the animals' hydration status. The mechanism of this electrometer is based upon the relationship between the red cell content and the electrical conductivity of the blood. The electrometer reading gave a significant correlation (p<0.001) with the venous haematocrit, thus indicating that the electrometer is a reliable apparatus for determining the animals' hydration status within seconds of venepuncture.  相似文献   

Studies on South African cardiac glycosides. I. Isolation of toxic principles of Homeria glauca (W. & E.) N.E. Br. and observations on their chemical and pharmacological properties     

T W Naudé  D J Potgieter 《The Onderstepoort journal of veterinary research》1971,38(4):255-277

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Prevalence and Aetiology of Mastitis in Cows from Two Major Ethiopian Dairies     

Workineh S  Bayleyegn M  Mekonnen H  Potgieter LN 《Tropical animal health and production》2002,34(1):19-25

The study was undertaken to determine the aetiology and prevalence of mastitis in hand-milked cows (n = 186) in two major Ethiopian dairies. The California Mastitis Test and culturing for bacteria revealed that 21.5% of the cows were clinically infected and 38.2% had subclinical mastitis. Most mastitis pathogens isolated from milk samples testing positive by the California Mastitis Test were Gram-positive cocci. Staphylococci constituted 57% of the isolates, of which the predominant cause of bovine mastitis was Staphylococcus aureus (40.5%). Other mastitis pathogens isolated include streptococci (16.5%), coliforms (9%) and corynebacteria (5%). Retrospective analysis of farm records indicated that mastitis was the second most important cause of culling and accounted for 27% of the cows removed from these two dairies.  相似文献   

Virucidal efficacy of four new disinfectants   总被引：5，自引：0，他引：5  

Eleraky NZ  Potgieter LN  Kennedy MA 《Journal of the American Animal Hospital Association》2002,38(3):231-234

Virucidal efficacy was evaluated for four recently available disinfectants: chlorine dioxide, potassium peroxymonosulfate, a quaternary ammonium compound, and citricidal (grapefruit extract). Sodium hypochlorite (3%) and tap water were used as positive and negative controls respectively. Feline herpesvirus, feline calicivirus, and feline parvovirus were exposed to the manufacturers' recommended dilutions of the evaluated disinfectants. Both chlorine dioxide and potassium peroxymonosulfate completely inactivated the three viruses used in this study. These disinfectants can aid in controlling nosocomial transmission of viruses with less of the deleterious effects of sodium hypochlorite. The quaternary ammonium compound evaluated in this study and citricidal were not effective against feline calicivirus and feline parvovirus.  相似文献   

Prevalence of bovine respiratory syncytial virus (BRSV) and bovine herpesvirus-4 (BHV-4) in cattle from Ethiopia     

Woldemeskel M  Kebede E  Yigezu L  Potgieter LN 《DTW. Deutsche tier?rztliche Wochenschrift》2000,107(11):464-466

A serological study was done to establish the occurrence and determine the prevalence of two important respiratory tract pathogens, bovine respiratory syncytial virus (BRSV) and bovine herpesvirus-4 (BHV-4), in cattle in Ethiopia. Prevalence rates for specific antibodies of 92.5% and 22.3% were recorded for BRSV and BHV-4, respectively. The presence of antibodies against these viruses in cattle from Ethiopia is recorded for the first time in this report. Our data suggests diseases caused by these viruses occur in Ethiopia but, perhaps because disease signs are not specific, they have not been recognized in the past.  相似文献   

Removal of Iron and Manganese from Water with a High Organic Carbon Loading. Part I: The Effect of Various Coagulants     

J. H. Potgieter  R. I. Mccrindle  Z. Sihlali  R. Schwarzer  N. BASSON 《Water, air, and soil pollution》2005,162(1-4):49-59

Iron and manganese removal from water is considered a routine operation in most potable water treatment plants. However, when there is dissolved organic carbon (DOC) present in the water source, it can significantly influence the success of iron and manganese removal. This investigation focused on the use of two different coagulants, alum and ferrichloride, used in conjunction with hydrogen peroxide as an oxidant, to reduce dissolved iron and manganese in water with a high DOC loading. The two coagulants behaved in a totally different manner during the treatment process, with alum being successful in reducing the iron content in the treated water, and ferrichloride yielding a good removal of the dissolved manganese present. Since these results were only possible in cases where hydrogen peroxide concentrations were also present, it was concluded that treatment with a single reagent would not yield the desired removal of contaminants from this specific water. Both treatment options significantly reduced the DOC levels in the water to below the desired concentration after treatment.  相似文献   

An investigation into the clinical pathological changes and serological response in horses experimentally infected with Babesia equi and Babesia caballi     

D T de Waal  J van Heerden  F T Potgieter 《The Onderstepoort journal of veterinary research》1987,54(4):561-568

Serologically negative horses, as determined with the indirect fluorescent antibody test (IFA), were infected with Babesia equi and 60 days later with Babesia caballi. The only clinical signs of disease observed in these animals were a febrile reaction and slight icterus. Haematological changes included a drop in haematocrit and haemoglobin concentration, as well as lowered platelet counts. The serum concentrations of albumin, iron and phosphorus were lowered. Mildly elevated serum bilirubin and fibrinogen concentrations were observed. Antibody titres were determined with the IFA and complement fixation (CF) tests. Antibodies to B. equi were first detected between Days 10-19 and 12-38 with the IFA and CF test, respectively, while the corresponding IFA periods for B. caballi were 6-8 days after infection. The parasitaemia of both B. equi and B. caballi infections never reached the 1% level.  相似文献   

Experimental bovine respiratory tract disease with Haemophilus somnus   总被引：1，自引：0，他引：1  

L N Potgieter  R G Helman  W Greene  M A Breider  E T Thurber  R H Peetz 《Veterinary pathology》1988,25(2):124-130

Eight calves were inoculated into the bronchus with H. somnus. Thirteen calves were inoculated with bovine respiratory syncytial virus (BRSV) and 8 days later with H. somnus. All calves developed necrotizing, suppurative, lobular bronchopneumonia and pleuritis. Clinical signs of disease and pneumonic lesions were significantly more severe in calves that were sequentially inoculated with BRSV followed by H. somnus. Pneumonic lesions in the inoculated calves were similar to those described for naturally occurring H. somnus-associated respiratory tract disease. Control calves inoculated with BRSV alone or sham-inoculated with medium did not develop clinical signs of respiratory tract disease. The BRSV-inoculated control calves developed minimal pneumonic lesions.  相似文献   

The transovarial transmission of Babesia trautmanni by Rhipicephalus simus to domestic pigs.     

D T De Waal  L M Lopez Rebollar  F T Potgieter 《The Onderstepoort journal of veterinary research》1992,59(3):219-221

Rhipicephalus simus was, for the first time, experimentally proven to be a transovarial vector of Babesia trautmanni of domestic pigs. The nymphal and adult progeny of experimentally infected female ticks transmitted the infection to 2 susceptible splenectomized pigs. Features of the infection included a prepatent period of 6-8 days post-tick infestation, a febrile reaction for 3 days and a maximum parasitaemia score of 15 (more than 6 parasites per 300 red blood cells). Other clinical signs in both pigs were mild inappetence and listlessness. Both pigs recovered without any antibabesial therapy.  相似文献