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Kaur Prabhdeep Filia G. Singh S. V. Patil P. K. Sandhu K. S. 《Tropical animal health and production》2010,42(5):1031-1035
Genotyping of Mycobacterium avium subspecies paratuberculosis (MAP) is important for precise classification of bacterium and for understanding the molecular epidemiology. The present
study reports detection and typing of the MAP from milk. On the basis of clinical signs of diarrhea and/or weakness, the dairy
animals suspected for Johne’s disease were screened by Ziehl–Neelsen staining of fecal samples. The milk samples from 13 selected
animals were processed for DNA extraction and direct IS900 polymerase chain reaction (PCR). MAP identified by IS900 PCR was
genotyped using IS1311 PCR-restriction endonuclease analysis (REA). IS900 milk PCR revealed 30.8% animals positive for MAP,
including 40% of the moderate and 50% of the heavy fecal shedders. All infected animals showed Bison type MAP in IS1311 PCR-REA.
IS900 PCR can be used for screening of milk for MAP; however, the method needs to be evaluated for subclinical cases. IS1311
PCR-REA results indicated the predominance of Bison type MAP in the dairy animals of this region. 相似文献
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