Digital mapping of soil salinity at various depths using an EM38     

Bhaskar Narjary  Murli Dhar Meena  Satyendra Kumar  Sushil Kumar Kamra  Dinesh Kumar Sharma  John Triantafilis 《Soil Use and Management》2019,35(2):232-244

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Blindness associated with toxoplasmosis in canaries.     

M C Vickers  W J Hartley  R W Mason  J P Dubey  L Schollam 《Journal of the American Veterinary Medical Association》1992,200(11):1723-1725

Seven of 30 canaries in an aviary in New Zealand developed ophthalmic problems. Clinically, 5 birds had unilateral and 2 birds had bilateral lesions characterized by conjunctivitis, crusty exudates on eyelids, and collapse of the eyeball. Microscopic lesions in 12 of 14 eyes examined included inflammation of the choroid and retina, with osseous replacement of the globe in some. Numerous Toxoplasma gondii tachyzoites were seen in the detached retina and vitreous humor of acutely affected birds. The diagnosis of toxoplasmosis was confirmed by immunohistochemical staining with T gondii antiserum. Affected birds had encephalitis, and T gondii was localized in the brains of these by immunohistochemical examination and by use of bioassays in mice. Toxoplasmosis should be considered in differential diagnosis of ophthalmitis in canaries.  相似文献   

The extent of genetic diversity among Vanilla species: Comparative results for RAPD and ISSR     

Praveen C. Verma  Debasis Chakrabarty  Satya Narayan Jena  Devesh K. Mishra  Pradhyumna K. Singh  Samir V. Sawant  Rakesh Tuli 《Industrial Crops and Products》2009,29(2-3):581-589

Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop.The present study investigated the genetic diversity among nine leafy- and leaf-less Vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0–66% and 0–81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by the cluster analysis. Specific groupings were revealed by each cluster analysis with slight variation between two different markers. Among the nine species studied, V. planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V. aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species.  相似文献   

Prevalence of Toxoplasma gondii infection in raccoons.     

J P Dubey  A N Hamir  C A Hanlon  C E Rupprecht 《Journal of the American Veterinary Medical Association》1992,200(4):534-536

Serum samples from 427 raccoons (93 from Pennsylvania, 45 from New Jersey, 72 from South Carolina, 68 from Virginia, 30 from Iowa, and 119 from Ohio) were evaluated for Toxoplasma gondii antibodies in dilutions of 1:25, 1:50, and 1:500. The distribution of T gondii antibody titers was less than 1:25 for 212 raccoons (49.6%), 1:25 for 34 raccoons (7.9%), 1:50 for 117 raccoons (27.4%), and greater than or equal to 1:500 for 64 raccoons (14.9%). Tissue cysts were seen in the liver, and tachyzoites were in the brain of a raccoon with abnormal neurologic signs and concurrent infection with canine distemper virus. Organisms in the liver were stained with anti-T gondii serum, and the raccoon had a T gondii titer of 1:160 in the agglutination test.  相似文献   

Toxoplasma gondii antibodies in exotic wild felids from Brazilian zoos.     

J C Silva  S Ogassawara  M F Marvulo  J S Ferreira-Neto  J P Dubey 《Journal of zoo and wildlife medicine》2001,32(3):349-351

Serum samples from 37 captive exotic felids in 12 zoos from six Brazilian states were assayed for antibodies to Toxoplasma gondii by the modified agglutination test using formalin-fixed whole tachyzoites. Titers greater than or equal to 1:20 were considered positive. Antibodies to T. gondii were found in 24 of 37 (64.9%) felids, including one European lynx (Lynx lynx), two jungle cats (Felis chaus), two servals (Leptailurus serval), two tigers (Panthera tigris), three leopards (Panthera pardus), and 14 of 27 lions (Panthera leo). This is the first serologic analysis for T. gondii infection in exotic wild felids from Brazilian zoos.  相似文献   

Ultrastructure of sarcocysts from water buffalo in India     

J P Dubey  C A Speer  H L Shah 《Veterinary parasitology》1989,34(1-2):149-152

The ultrastructure of sarcocysts of macro- and microscopic species of Sarcocystis was compared from naturally infected water buffalo from India. Grossly visible sarcocysts had walls consisting of cauliflower-like villar protrusions, typical of S. fusiformis. The sarcocyst wall of the microscopic species of Sarcocystis was 6.4 microns thick and consisted of tightly packed conical villar protrusions that were 9.6 microns long and 3.7 microns wide at the base. At approximately 3 microns above the base, the distal two-thirds of the villar protrusion became conical shaped and was bent laterally at an angle of 45 degrees to the sarcocyst surface. The granular layer beneath the villar protrusions was 0.9 microns thick. In S. levinei the granular layer was 1.9 microns thick, the villar protrusions were narrow and it had a highly undulating primary cyst wall. Whether the microscopic S. levinei-like sarcocysts of Indian and Malaysian water buffalo are distinct species of Sarcocystis will require further investigation.  相似文献   

Primary sulfates in atmospheric sulfates: estimation by oxygen isotope ratio measurements     

Holt BD  Kumar R  Cunningham PT 《Science (New York, N.Y.)》1982,217(4554):51-53

The relative amounts of primary and secondary sulfates in atmospheric aerosols and precipitation can be estimated from measurements of the stable oxygen isotope ratios. The oxygen-18 content of sulfates formed in power plant stack gases before emission into the atmosphere is significantly higher than that of sulfates formed from sulfur dioxide after emission. Results show that 20 to 30 percent of the sulfates in rain and snow at Argonne, Illinois, are of primary origin.  相似文献   

First report of gummy stem blight caused by Didymella bryoniae on muskmelon (Cucumis melo) in India     

J. Sudisha  T. Vasanth Kumar  S. R. Niranjana  H. Shekar Shetty 《Plant pathology》2004,53(4):533-533

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Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats          下载免费PDF全文

M Narasimhaiah  A Arunachalam  S Sellappan  VK Mayasula  PR Guvvala  SK Ghosh  V Chandra  J Ghosh  H Kumar 《Reproduction in domestic animals》2018,53(3):644-654

Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (p < .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (p < .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.  相似文献   

Spermatogenesis and related plasma androgen and progestin level in wild male golden mahseer,Tor putitora (Hamilton, 1822), during the spawning season     

Neetu Shahi  Sumanta Kumar Mallik  Jyoti Pande  Partha Das  Atul Kumar Singh 《Fish physiology and biochemistry》2015,41(4):909-920

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