全文获取类型
收费全文 | 716篇 |
免费 | 81篇 |
专业分类
林业 | 48篇 |
农学 | 33篇 |
122篇 | |
综合类 | 41篇 |
农作物 | 28篇 |
水产渔业 | 106篇 |
畜牧兽医 | 367篇 |
园艺 | 14篇 |
植物保护 | 38篇 |
出版年
2023年 | 5篇 |
2022年 | 12篇 |
2021年 | 19篇 |
2020年 | 23篇 |
2019年 | 32篇 |
2018年 | 35篇 |
2017年 | 43篇 |
2016年 | 33篇 |
2015年 | 18篇 |
2014年 | 34篇 |
2013年 | 42篇 |
2012年 | 40篇 |
2011年 | 54篇 |
2010年 | 34篇 |
2009年 | 31篇 |
2008年 | 31篇 |
2007年 | 32篇 |
2006年 | 30篇 |
2005年 | 22篇 |
2004年 | 23篇 |
2003年 | 29篇 |
2002年 | 18篇 |
2001年 | 14篇 |
2000年 | 17篇 |
1999年 | 9篇 |
1998年 | 8篇 |
1997年 | 18篇 |
1996年 | 3篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 15篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 10篇 |
1988年 | 7篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
排序方式: 共有797条查询结果,搜索用时 31 毫秒
1.
Vimal Nair Min Cheol Kim James A. Golen Arnold L. Rheingold Gabriel A. Castro Paul R. Jensen William Fenical 《Marine drugs》2020,18(11)
A new cytotoxic thiodepsipeptide, verrucosamide (1), was isolated along with the known, related cyclic peptide thiocoraline, from the extract of a marine-derived actinomycete, a Verrucosispora sp., our strain CNX-026. The new peptide, which is composed of two rare seven-membered 1,4-thiazepane rings, was elucidated by a combination of spectral methods and the absolute configuration was determined by a single X-ray diffraction study. Verrucosamide (1) showed moderate cytotoxicity and selectivity in the NCI 60 cell line bioassay. The most susceptible cell lines were MDA-MB-468 breast carcinoma with an LD50 of 1.26 µM, and COLO 205 colon adenocarcinoma with an LD50 of 1.4 µM. Also isolated along with verrucosamide were three small 3-hydroxy(alkoxy)-quinaldic acid derivatives that appear to be products of the same biosynthetic pathway. 相似文献
2.
One hundred two fecal specimens from psittacine birds submitted to Veterinary Laboratory Services of the California Department of Food and Agriculture at Petaluma were screened for Chlamydia psittaci by a direct immunofluorescence assay using a fluorescein-labeled monoclonal antibody conjugate specific for Chlamydia sp. Results were compared with those obtained by isolation of chlamydia in cultures of McCoy mouse cells. The relative specificity of the direct fluorescent antibody test on fecal smears was 98.9% and the relative sensitivity was 62.5%. The results of this study suggested that the direct fluorescent antibody test was highly specific, and it proved to be a useful same-day antemortem diagnostic test for birds with symptomatic chlamydial infection. The use of centrifugation in the cell culture assay was found to significantly enhance the level of chlamydial infection in cell culture. 相似文献
3.
SUMMARY Twenty-four of 57 calves fed a diet containing 33% cotton seed meal (CSM) died between 7 and 15 weeks of age. Initial deaths were not accompanied by premonitory signs, but after CSM withdrawal most calves developed rough coats, anorexia, weakness, ascites and subcutaneous oedema. Those that died had large volumes of serous fluid in the body cavities, hard livers of ‘nutmeg’ appearance, and pulmonary congestion. Histopathologically the livers showed periacinar necrosis in acute cases and periacinar fibrosis in chronic cases. Lungs from several calves had oedema, haemosiderosis and fibrosis in some pulmonary vessels. Atrophy of myocardial fibres was present in most cases. The concentration of free gossypol in the diet was 100 to 220 mg/kg. Ante-mortem and post-mortem findings supported a diagnosis of gossypol poisoning. The deaths continued for 4 weeks after withdrawal of CSM from the diet. 相似文献
4.
Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758) 下载免费PDF全文
GL Lima VB Luz LF Lima RMP Rocha SV Castro TS Castelo APR Rodrigues JR Figueiredo AR Silva 《Reproduction in domestic animals》2018,53(4):880-888
The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions. 相似文献
5.
RO Pinho BS Camilo DMA Lima FAC Villadiego JCM Vergara HH Shiomi RE Cardoso PS Lopes JD Guimarães 《Reproduction in domestic animals》2018,53(2):393-400
The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty‐six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles. The groups of animals that were evaluated showed no difference in the main semen parameters that were evaluated, except for the sperm volume, concentration of the ejaculated sperm and the supravital staining; the lowest figures were for the animals from the Piau breed (group 1). In relation to the testicular biometrics, Duroc animals (group 4) had a greater scrotal width compared to the other groups. But when we assessed the intensity of pixels of the testicles, there was a difference between groups. The groups 2 (finishing animals), 3 (Pietrain) and 4 had no difference between themselves. Group 3 had greater pixel intensity in relation to group 1. Of the 26 animals studied, five showed an abnormality during ultrasound evaluation, like hydrocele, hyperechoic mass in the testicular parenchyma, cyst in the head of the epididymis and the presence of fluid in the head and tail of the epididymis. The various animal groups studied did not differ in the principal reproductive parameters evaluated, showing that despite the great variability of reproductive traits between breeds and within the same breed, the breeding soundness evaluation, the more complete it is, is essential for the selection of breeders and the ultrasonography of the reproductive system becomes an important addition in this examination. 相似文献
6.
Carbonari Caio Antonio Gomes Giovanna Larissa Gimenes Cotrick Krenchinski Fábio Henrique Simões Plinio Saulo Batista de Castro Edicarlos Velini Edivaldo Domingues 《New Forests》2020,51(4):723-737
New Forests - Eucalyptus harvests generate residues that remain on the soil and affect the efficacy of preemergent herbicides in the following planting cycle. The objective of this work was to... 相似文献
7.
S. Leal‐Soto R. Barraza‐Guardado R. Castro‐Longoria J. Chvez‐Villalba F. Hoyos‐Chirez 《Journal of the World Aquaculture Society》2011,42(6):789-800
Pen shells are economically important and most are threatened by overexploitation and habitat degradation. Cultivation represents an alternative for preserving the resource and increasing the production. Atrina maura was cultivated in a suspension system which was followed by a bottom phase. Samples of pen shells and tissues were collected monthly to measure absolute growth, shell height increase, and an adductor muscle index. After the trials, adults shell height was 195 ± 10 mm, total weight was 223 ± 28 g, and mean shell height increase was 0.81 cm/mo. Protective devices were vital to avoid predation by crabs and fish; survival was 90% in the suspension phase and 70% in the bottom phase. Variations in the muscle index were apparently related to reproduction, suggesting activity (partial spawning) from August 2008 to April 2009 in juveniles and a major spawning in adults during August 2009. Adductor muscles reached a mean of 21 g after 20 mo of cultivation, but muscle weights decreased significantly after reproduction (August to November 2009). Results indicated that A. maura is suitable for aquaculture and the cultivation system adopted here should be tested in pilot‐scale ventures. This system may work with other species of pen shells. 相似文献
8.
Analgesic efficacy of an oral transmucosal spray formulation of meloxicam alone or in combination with tramadol in cats with naturally occurring osteoarthritis 下载免费PDF全文
9.
10.
Microdialysis measurements of equine lamellar perfusion and energy metabolism in response to physical and pharmacological manipulations of blood flow 下载免费PDF全文