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1.
Microbial activities impact arsenic (As) transformation in mine tailings substantially,yet little is understood on the functional diversity of As metabolism genes.This study explored this issue using a metagenomic approach coupled by a local BLASTN procedure established in our recent studies.An assembled metagenome,recovered from hypersaline and sulfidic mine tailings,was screened for As metabolism genes aioA,arrA,arsC and arsM.This was done using a local BLASTN procedure against databases of the As metabolism genes built in this study.Putative As metabolism genes detected in the assembled metagenome included 11 arsM,20 arsC and 1 arrA full-length sequences.Together with the rRNA-based phylogenetic profiling results,a picture depicting microbial As cycling in the tailings to the genus level was obtained.It was found that most of the dominant genera in the tailings potentially harboured the genes for As reduction and/or methylation.In particular,a typical pyrite-eater present in the tailings,Thioalkalivibrio sp.,was found to harbour not only arsC and arsM,but also arrA.These results highlight the unexpected diversity of As metabolism genes in the tailings,especially considering the extremely low species diversity therein.The microbial As cycling picture established here has potential use for guiding the purposeful manipulation of As biogeochemistry in the tailings. 相似文献
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Modelling the effect of weed-seed distribution in the soil profile on seedling emergence 总被引:3,自引:0,他引:3
Experiments to monitor weed seedling emergence from various soil depths were begun in 1989. Recently shed seeds were buried in either narrow bands at a range of depths in the soil or mixed evenly between the soil surface and each of these depths. Total emergence of Stellaria media (L.) Vill., Matricaria spp., Veronica persica Poiret, Veronica arvensis L. and Polygonum aviculare L., declined with increasing depth of burial. A similar pattern was observed for Chenopodium album L. with the exception of emergence from the surface layer. The reduction in emergence with increasing soil depth was greater for seeds in the narrow-banded treatments than for those in broad layers. A model was developed using the data from the narrow-banded treatments to predict emergence from distributions over broad layers. Predictions from the model closely agree with the data from the present broad-layer treatments and those from a similar experiment made in the 1960s. By including the effects of depth of seed burial on seedling emergence, this model could be combined with models that determine the effects of cultivation on seed distribution, and therefore improve precision of predictions of seedling emergence from the seedbank. 相似文献
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D. E. JACOBS M. T. FOX J. G. PILKINGTON D. B. ROSS W. G. RYAN† 《Journal of veterinary pharmacology and therapeutics》1989,12(4):444-450
Seeder calves infected with Dictyocaulus viviparus were used to contaminate a field divided into three similar paddocks. Twenty-four autumn-born calves were allocated to three matched groups; one group was given topical ivermectin treatments (0.5 mg/kg) at 3, 8 and 13 weeks after turnout (Day 0); each member of a second group was given an oxfendazole pulse-release intraruminal device (OPRB) at turnout; while a third group was kept as untreated controls to monitor the natural epidemiological pattern of events. Severe pasteurella pneumonia exacerbated by lungworm infection occurred in the controls after Day 24. Two died and repeated doses of antibiotic and anthelmintic therapy were necessary to save the remainder. Clinical signs were much milder in the ivermectin and OPRB groups and resolved with only a single dose of antibiotic. The OPRB group excreted some lungworm larvae at this time, but none was detected in the faeces of the ivermectin group during the grazing season. At housing, five calves from each group and four lungworm-naive calves were challenged with D. viviparus larvae. The infection became patent in all challenge-control calves, but no larvae were passed by any of the trial animals. Post-mortem worm-counts revealed percentage takes for the challenge controls, trial controls, ivermectin and OPRB groups of 16.7, 0.01, 0.9 and 0.2, respectively. All trial groups had therefore developed a substantial immunity. 相似文献
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1. Vigilance and feeding behaviour of male and female adult breeding ostriches were recorded to determine feeding and scanning bout lengths, a time budget and the pattern of vigilance immediately after food was provided. 2. Males were more vigilant and fed for shorter periods than females immediately after concentrate food was delivered but not throughout the whole day. 3. Most interscan periods of males were below 40 s with a maximum of 90 s compared with most interscan periods of females lasting less than 70 s with a maximum of 160 s. 4. Gender differences in behaviour are attributed to increased male vigilance for predators and/or conspecifics, and increased female feeding required for egg production and greater opportunity to feed because of male vigilance. 相似文献
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Abstract. The effectiveness of xylocaine anaesthesia, potentiated with sodium bicarbonate, was investigated on the altiplano cyprinid, Algansea lacustris Steindachner. The drug gave excellent sedation, handling and recovery and was effective at doses between 50 and 300 mg/l in 1 g/l sodium bicarbonate. 相似文献
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