Porcine reproductive and respiratory syndrome virus detection in Thailand during 2005–2010 in relation to clinical problems, pig types, regions, and seasons     

Padet Tummaruk  Pannin Surapat  Sutharat Sriariyakun  Oraphan Seemakram  Em-on Olanratmanee  Rachod Tantilertcharoen  Roongroj Thanawongnuwech 《Tropical animal health and production》2013,45(3):771-779

The objectives of the present study were to determine the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand between 2005 and 2010. The study was conducted by retrospectively investigating the detection of PRRSV from different pig types including boars, sows, piglets, nursery pigs, and fattening pigs from six regions of Thailand, i.e., the northern, eastern, northeastern, central, western, and southern parts. The data were obtained from cases submitted to the Chulalongkorn University Veterinary Diagnostic Laboratory for PRRSV detection between 2005 and 2010. Frequency analyses and generalized linear models were used to evaluate the prevalence of PRRSV in relation to various factors. In total, 2,273 tissues (n?=?636), semen (n?=?210) and serum (n?=?1,427) samples were included. PRRSV was detected in 32.6 % (740/2,273) of the pigs. The virus was found in 43.1 %, 15.7 %, and 30.3 % in the tissues, semen, and serum samples, respectively (P?<?0.001). The prevalence of PRRSV was highest in 2005 (43.6 %) and lowest in 2009 (23.6 %) (P?<?0.001). The prevalence of PRRSV was highest in nursery pigs (43.7 %) and lowest in boars (15.4 %) (P?<?0.001). The prevalence of PRRSV in the hot season (34.9 %) was higher than that found in the cool season (28.1 %, P?=?0.018) but did not differ significantly compared to rainy season (34.0 %, P?=?0.486). The strain of PRRSV isolated in the present study was genotype 2 (54.5 %), genotype 1 (31.0 %), and mixed genotypes (14.5 %). It can be concluded that PRRSV was detected in the tissue samples more frequently than the semen and serum samples. The prevalence of PRRSV was high in the nursery pigs. A high prevalence of PRRSV was found in the hot season, indicating that climatic factors may also contribute to the prevalence of PRRSV in Thailand. Of all the PRRSV detected, 31.0 %, 54.5 %, and 14.5 % belonged to genotype 1, genotype 2, and mixed genotypes, respectively.  相似文献   

The inactivation of avian influenza virus subtype H5N1 isolated from chickens in Thailand by chemical and physical treatments     

Suwarak Wanaratana  Rachod Tantilertcharoen  Jiroj Sasipreeyajan  Somsak Pakpinyo 《Veterinary microbiology》2010,140(1-2):43-48

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Seroprevalence of porcine reproductive and respiratory syndrome,Aujeszky’s disease,and porcine parvovirus in replacement gilts in Thailand   总被引：2，自引：1，他引：1  

Tummaruk P  Tantilertcharoen R 《Tropical animal health and production》2012,44(5):983-989

The present study investigated the seroprevalence of porcine reproductive and respiratory syndrome virus, Aujeszky’s disease virus (ADV), and porcine parvovirus (PPV) in replacement gilts from selected five swine herds in Thailand. The study consisted of three parts. First, a retrospective data analysis on the seroprevalence of porcine reproductive and respiratory syndrome virus (PRRSV) and ADV glycoprotein I (gI) in gilts, sows, boars, nursery, and fattening pigs in five herds (n = 7,030). Second, a cross-sectional study on seroprevalence of PRRSV, ADV, and PPV (n = 200) in replacement gilts. Last, the seroprevalence of PRRSV, ADV, and PPV in gilts culled due to reproductive failure (n = 166). Across the herds, the seroprevalence of PRRSV and ADV was 79.3% and 5.3%, respectively. The cross-sectional study revealed that 87.5%, 4.0%, and 99.0% of the replacement gilts were infected with PRRSV, ADV, and PPV, respectively. In the gilts culled due to reproductive failure, the seroprevalence of PRRSV, ADV, and PPV was 73.5%, 28.3%, and 86.0%, respectively. Of these culled gilts, 75.5% had been infected with at least two viruses and 18.9% had been infected with all three viruses. It could be concluded that most of the replacement gilts were exposed to PRRSV (84%), PPV (97%), and ADV (4%) before entering the breeding house. PPV was an enzootic disease among the selected herds. The prevalence of ADV was higher in gilts culled due to reproductive disturbance than in the healthy gilts.  相似文献   

Comparative study of pandemic (H1N1) 2009, swine H1N1, and avian H3N2 influenza viral infections in quails     

Aunyaratana Thontiravong  Suwarak Wannaratana  Rachod Tantilertcharoen  Duangduean Prakairungnamthip  Ranida Tuanudom  Jiroj Sasipreeyajan  Somsak Pakpinyo  Alongkorn Amonsin  Pravina Kitikoon  Kanisak Oraveerakul 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):395-403

Quail has been proposed to be an intermediate host of influenza A viruses. However, information on the susceptibility and pathogenicity of pandemic H1N1 2009 (pH1N1) and swine influenza viruses in quails is limited. In this study, the pathogenicity, virus shedding, and transmission characteristics of pH1N1, swine H1N1 (swH1N1), and avian H3N2 (dkH3N2) influenza viruses in quails was examined. Three groups of 15 quails were inoculated with each virus and evaluated for clinical signs, virus shedding and transmission, pathological changes, and serological responses. None of the 75 inoculated (n = 45), contact exposed (n = 15), or negative control (n = 15) quails developed any clinical signs. In contrast to the low virus shedding titers observed from the swH1N1-inoculated quails, birds inoculated with dkH3N2 and pH1N1 shed relatively high titers of virus predominantly from the respiratory tract until 5 and 7 DPI, respectively, that were rarely transmitted to the contact quails. Gross and histopathological lesions were observed in the respiratory and intestinal tracts of quail inoculated with either pH1N1 or dkH3N2, indicating that these viruses were more pathogenic than swH1N1. Sero-conversions were detected 7 DPI in two out of five pH1N1-inoculated quails, three out of five quails inoculated with swH1N1, and four out of five swH1N1-infected contact birds. Taken together, this study demonstrated that quails were more susceptible to infection with pH1N1 and dkH3N2 than swH1N1.  相似文献   

Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand     

Kiatipattanasakul-Banlunara W  Tantilertcharoen R  Suzuki K  Albarenque SM  Thanawongnuwech R  Nakayama H  Doi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(5):449-452

A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.  相似文献   

Psittacine beak and feather disease in three captive sulphur-crested cockatoos (Cacatua galerita) in Thailand     

Kiatipattanasakul-Banlunara W  Tantileartcharoen R  Katayama K  Suzuki K  Lekdumrogsak T  Nakayama H  Doi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):527-529

Three sulphur-crested cockatoos (Cacatua galerita) were diagnosed as psittacine beak and feather disease (PBFD). Histopathology of the feather pulp and follicles showed intracytoplasmic botryoid clusters or granular inclusion bodies in epithelial cells and macrophages. Electron microscopy revealed multiple cytoplasmic clusters of electron dense viral particles corresponding to the inclusions. PBFD virus (circovirus) DNA-specific product was detected from formalin-fixed paraffin-embedded feathers by nested polymerase chain reaction (PCR) method.  相似文献