全文获取类型
收费全文 | 877篇 |
免费 | 37篇 |
国内免费 | 93篇 |
专业分类
林业 | 36篇 |
农学 | 59篇 |
基础科学 | 40篇 |
81篇 | |
综合类 | 389篇 |
农作物 | 76篇 |
水产渔业 | 21篇 |
畜牧兽医 | 177篇 |
园艺 | 74篇 |
植物保护 | 54篇 |
出版年
2024年 | 3篇 |
2023年 | 8篇 |
2022年 | 40篇 |
2021年 | 40篇 |
2020年 | 25篇 |
2019年 | 35篇 |
2018年 | 31篇 |
2017年 | 45篇 |
2016年 | 25篇 |
2015年 | 50篇 |
2014年 | 53篇 |
2013年 | 39篇 |
2012年 | 68篇 |
2011年 | 79篇 |
2010年 | 63篇 |
2009年 | 85篇 |
2008年 | 64篇 |
2007年 | 53篇 |
2006年 | 52篇 |
2005年 | 32篇 |
2004年 | 16篇 |
2003年 | 12篇 |
2002年 | 20篇 |
2001年 | 15篇 |
2000年 | 8篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 9篇 |
1995年 | 1篇 |
1994年 | 6篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1958年 | 2篇 |
1956年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有1007条查询结果,搜索用时 609 毫秒
1.
2.
3.
AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P<0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps. 相似文献
4.
Two disorders of almond, know as 'stem pitting’and‘graft union necrosis (black line)', were described years ago from Puglia (southern Italy). The etiology of these diseases was uncertain, the causal agent unknown and reliable diagnostic methods were not available. Investigations were therefore carried out to identify Prunus species and/or cultivars as possible indicators for a quick and reliable diagnosis. In 1990, two different almond sources (cv. Filippo Ceo) affected by black line and stem pitting were budded onto seedlings of P. persica, P. cerasifera, P. amygdalus cv. Don Carlo, GF305, self-rooted cuttings of GF677 (P. persica×P. amygdalus) and onto Don Carlo and GF305 seedlings that had been already grafted with almond cvs Genco, Tuono and Filippo Ceo. At least two inoculated plants were inspected each year for the presence of symptoms on the woody cylinder after removal of the cortex. Stem pitting developed on P. persica and P. cerasifera seedlings during the first year after graft inoculation. On the grafted plants that had been inoculated, pitting and grooving of the wood were much more pronounced in cvs Filippo Ceo and Genco, with a higher incidence when GF305 was used as rootstock. Clear symptoms were observed (from the first year in cvs Tuono and Genco), regardless of the rootstock, all along the junction line and often not associated with pitting of the rootstock and scion. 相似文献
5.
JIANG Xun ZENG Yao-ying HE Xian-hui XU Li-hui DI Jing-fang FENG Zheng ZHAO Jing-xian WANG Qing WANG Tong SHI Jian-bo 《园艺学报》2004,20(6):924-928
AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage. 相似文献
6.
DU Yi-mei TANG Ming LIU Chang-jin HONG Zhi-gang KE Qin-mei DI Jiu-fang LUO Hong-yan HU Mou-xian HU Xin-wu XI Jiao-ya TANG Bi Jurgen Hescheler 《园艺学报》2004,20(9):1537-1541
AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV. 相似文献
7.
V. L. Savage L. A. Cudmore C. M. Russell D. I. Railton A. P. Begg N. M. Collins A. R. Adkins 《Equine Veterinary Education》2018,30(8):403-408
Cystic lymphangiomas are rare malformations of the lymphatics that result in the formation of a cystic mass. Where multiple cysts are seen, the condition is termed cystic lymphangiomatosis. This case describes the diagnosis and unique management of cystic lymphangiomatosis in a 10‐day‐old Thoroughbred foal. Ultrasonography, histopathology and laparoscopy were essential for diagnosis and appreciation of the extent of disease. Ultimately, the cystic lymphangiomatosis was so extensive in this foal that complete surgical excision was considered impossible and the presence of adhesions within the abdomen indicated a very poor long‐term prognosis; the owners elected for euthanasia at age 14 weeks. Although rare, lymphangioma and lymphangiomatosis should be considered as a differential diagnosis for an intra‐abdominal mass in a young horse. 相似文献
8.
极细链格孢激活蛋白对大豆光合特性、叶绿素荧光参数及产量的影响 总被引:1,自引:0,他引:1
为探讨激活蛋白对大豆生长、产量的影响,以大豆冀豆17为研究对象,利用光合仪与称重法测量了不同浓度极细链格孢激活蛋白(1,2,3,4,5,6μg/m L)处理后大豆的光合特性、叶绿素荧光参数及产量。结果表明,随着施用的极细链格孢激活蛋白浓度的增加,大豆叶绿素含量、净光合速率、气孔导度、蒸腾速率、最大光化学量子效率(Fv/Fm)、有效量子效率(ΦPSⅡ)和光化学猝灭系数(q P)呈先上升后下降的趋势。施用1,2,3,4μg/m L浓度的极细链格孢激活蛋白不仅提高了大豆叶绿素含量,而且增加了大豆叶片的净光合速率、气孔导度、蒸腾速率,同时还能提高叶绿素荧光的最大光化学量子效率(Fv/Fm)、有效量子效率(ΦPSⅡ)和光化学猝灭系数(q P)。其中以3,4μg/m L浓度的处理效果最好。施用更高浓度的激活蛋白(5,6μg/m L),上述光合特性与叶绿素荧光促进效果下降或者消失。施用中浓度激活蛋白(3,4μg/m L)大豆产量增加了11.21%~14.76%。结果表明,喷洒合适浓度的极细链格孢激活蛋白是一种促进大豆增产的有效措施。 相似文献
9.
10.
为研究硒浸种对春小麦种子活力和幼苗生长特性的影响,分别设置0,0.5,1.0,2.5,3.5,5.0,10.0,50.0 mg/L共8个亚硒酸钠溶液浓度,通过发芽试验,比较研究了不同浓度的亚硒酸钠浸种对春小麦种子萌发和幼苗生长的影响.结果表明:随着亚硒酸钠溶液浓度升高,春小麦种子的发芽率、发芽势、发芽指数、活力指数、幼苗和根长以及干鲜重等指标均呈先升高后下降的趋势;1.0 mg/L的亚硒酸钠溶液对小麦种子萌发和幼苗生长有促进作用;随着亚硒酸钠浓度的升高(≥2.5 mg/L),其各项指标显著下降,并对根和苗的生长表现出抑制作用;因此在实际生产中,掌握合适的硒浸种浓度非常关键. 相似文献