What Is Your Diagnosis?     

Walton RM  Thrall MA  Wheeler S 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(4):117-118

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Experimental infection of infectious myonecrosis virus (IMNV) in the Pacific white shrimp Litopenaeus vannamei (Boone, 1931)     

Suzianny Maria Bezerra Cabral da Silva  João Luís Rocha  Pedro Carlos Cunha Martins  Alfredo Olivera Gálvez  Fernando Leandro dos Santos  Humber Agrelli Andrade  Maria Raquel Moura Coimbra 《Aquaculture International》2015,23(2):563-576

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Fortification of Wheat Dough with Calcium and Magnesium Ions Affects Empirical Rheological Properties     

Georgia Ane Raquel Sehn  Amanda de Cssia Nogueira  Eveline Lopes Almeida  Yoon Kil Chang  Caroline Joy Steel 《Cereal Chemistry》2015,92(4):405-410

This study evaluated the influence of calcium and magnesium ions on the empirical rheological properties of wheat flour to verify possible effects of these ions on processing because, in addition to their nutritional importance, they are also responsible for water hardness. Calcium (0–1.30 g/100 g) and magnesium (0–0.34 g/100 g) ions from sulfate salts were added to wheat flour, according to a central composite rotatable design. The farinograph and extensigraph properties of wheat flour and its mixtures were evaluated. The results were analyzed by response surface methodology. Calcium ions stood out for increasing water absorption, decreasing mixing stability, and producing a delayed effect on dough extensibility (reduced at 135 min). Magnesium ions influenced most flour rheological properties in a similar manner to oxidizing agents (increased dough stability, increased resistance to extension, and reduced extensibility), thus proving to be a possible replacement agent for these additives. An interaction effect of the combined calcium and magnesium ions was observed on dough development time. The results showed that effects on processing can occur when wheat flour fortification is made, and adaptations on wheat flour specifications, product formulation, and processing parameters may be required.  相似文献   

Validation of new automated turbidimetric immunoassays for the measurement of haptoglobin and inter-α-trypsin inhibitor heavy chain H4 specific for the bovine species     

Anna Bassols  José Angel Robles-Guirado  Laura Arroyo  Lourdes Soler  Natalia García  Raquel Pato  Raquel Peña  Yolanda Saco  Ramon Armengol  Fermín Lampreave  María A. Alava  Francesca Canalias  Matilde Piñeiro 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(Z1):64-74

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Hepatozoon spp.: report of some cases in dogs in Brasília, Brazil     

Paludo GR  Dell'Porto A  de Castro e Trindade AR  McManus C  Friedman H 《Veterinary parasitology》2003,118(3-4):243-248

Canine hepatozoonosis is a disease caused by the tick-borne protozoan Hepatozoon spp. It has been reported in the United States, southern Europe, the Middle East, Africa and the Far East. In Brazil, canine hepatozoonosis is an emerging protozoal tick-borne disease, and is characterized by distinct clinical signs. The objective of this study was to analyze the laboratory findings of some hepatozoonosis cases in dogs in Brasília, Brazil, and their clinical signs. The animals of this experiment showed low parasitemia, similar to H. americanum, but the clinical signs presented were similar to H. canis. According to our observations and in agreement with O'Dwyer et al. [Vet. Parasitol. 94 (2001) 143], the Brazilian Hepatozoon appears more to resemble the species found in the eastern Hemisphere than with H. americanum of North America, or could be caused by a new species. Our data revealed that hepatozoonosis could be considered endemic in Brasília.  相似文献   

Evaluation of metabolic detoxifying enzyme activities and insecticide resistance in Frankliniella occidentalis     

Maymó AC  Cervera A  Sarabia R  Martínez-Pardo R  Garcerá MD 《Pest management science》2002,58(9):928-934

The western flower thrips Frankliniella occidentalis (Pergande) is a very significant pest of a number of different agricultural crops in the south-east of Spain. The importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (TSWV) which they transmit. The economic threshold is therefore almost zero, which enhances the problems of resistance management. The present work is part of a global project that attempts to evaluate the status of insecticide resistance in field populations of thrips obtained from several agricultural crops. We have studied, in either individual or pooled insects, some enzyme systems classically related to detoxification of insecticides: esterase and glutathione-S-transferase (GST). The activity of these enzymes from laboratory populations selected with various classes of insecticides has also been measured using several appropriate substrates. An increase in GST mean activity was found in two field-collected strains. Differences in frequency distributions of esterase and GST activities were found for both field-collected strains and for a laboratory strain selected with acrinathrin. These activities were compared with those of a wild-type reference strain.  相似文献   

Testing genetic determinism in rate of hoof growth in pigs using Bayes Factors     

Raquel Quintanilla  Luis Varona  Jos Luis Noguera 《Livestock Science》2006,105(1-3):50-56

Excessive growth and consequent deformity of hooves is a frequent disorder in some purebred pig populations. A test to detect possible genetic determinism related with this phenomenon was performed using the Bayes Factor (BF). Data were available for females from three purebred selection lines: Landrace (561 records), Pietrain (183) and Large White (225). Animals were scored in four categories, according to the overall growth rate of their hooves. A Bayesian analysis was performed separately for each line using a threshold model with a probit approach, and Bayes Factors between models with and without additive genetic effects were computed. Results from the three lines showed that models exhibiting genetic variability were much more probable than those that did not include a genetic component, with BF values of 312, 35 and 40 (and posterior probabilities of 0.99, 0.97 and 0.98), respectively, for the Landrace, Pietrain and Large White lines. Monte Carlo estimates of posterior means of heritabilities were medium to high (0.25, 0.41 and 0.38, respectively), and the highest posterior density region for heritability at 99% did not include zero in any of the three lines. These results allow us to conclude that genetic determinism has an important influence upon the rate of hoof growth in the pig. A potential genetic response can be achieved in the populations analysed, but further studies are needed to determine the genetic architecture of hoof growth disorders in pigs.  相似文献   

Genetic variation of Prunus avium in susceptibility to cherry leaf spot (Blumeriella jaapii) in spatially heterogeneous infected seed orchards     

Raquel Díaz  Rafael Zas  Josefa Fernández-López 《Annals of Forest Science》2007,64(1):21-30

Cherry leaf spot (Blumeriella jaapii (Rehm) Arx.) is a very serious disease of wild cherry (Prunus avium L.), which produces premature leaf defoliation and vigor decrease. In two clonal seed orchards of P. avium naturally infected by B. jaapii, spatial heterogeneity and autocorrelation of neighbor damage caused by cherry leaf spot impeded proper analysis of the fungus incidence. The iterative spatial analysis (ISA), based on variography and kriging, was successfully used to eliminate the effect of this spatial heterogeneity in analysis of genetic variation in susceptibility to B. jaapii. Significant differences among P. avium clones were found, with moderate to high broad-sense heritability estimates. Genetic by environment interactions, although significant, were not quantitatively important. A strong relationship between leaf spot susceptibility and bud burst was found. However, other factors must be affecting the genetic variation in leaf spot susceptibility, as differences among clones remained highly significant when considering the bud burst as a covariate in the genetic model.  相似文献   

Influence of diet and feeding strategy on the performance of nitrifying trickling filter,oxygen consumption and ammonia excretion of gilthead sea bream (Sparus aurata) raised in recirculating aquaculture systems     

Godoy-Olmos  Sergio  Jauralde  Ignacio  Monge-Ortiz  Raquel  Milián-Sorribes  María C.  Jover-Cerdá  Miguel  Tomás-Vidal  Ana  Martínez-Llorens  Silvia 《Aquaculture International》2022,30(2):581-606

Gilthead sea bream (Sparus aurata) was raised in six individual recirculating aquaculture systems (RAS) whose biofilters’ performance was analyzed. Fish were fed with three different diets (a control diet, a fishmeal-based diet (FM), and a plant meal-based diet (VM)) and with three different feeding strategies (manual feeding to apparent satiation, automatic feeding with restricted ration, and auto-demand feeding). For every combination of diet and feeding strategy, the mean oxygen consumption, ammonia excretion, and ammonia removal rate were determined. Fish fed with the VM diet consumed the most oxygen (20.06?±?1.80 gO₂ consumed kg^?1 day^?1). There were significant differences in ammonia excretion depending on the protein content and protein efficiency of the diet, as well as depending on feeding strategy, which in turn affected ammonia removal rates. Fish fed by auto-demand feeders led to the highest mean ammonia removal rate (0.10 gN-TAN removed m^?2 biofiltration area day^?1), while not leading to peaks of high ammonia concentration in water, which preserve fish welfare and growth.

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Evaluation of hepatitis E virus infection between different production systems of pigs in Brazil     

Marconni Victor da Costa Lana  Noemi Rovaris Gardinali  Raquel Aparecida Sales da Cruz  Letícya Lerner Lopes  Gustavo Sousa Silva  João Garcia Caramori Júnior  Anderson Castro Soares de Oliveira  Marcos de Almeida Souza  Edson Moleta Colodel  Amauri Alcindo Alfieri  Caroline Argenta Pescador 《Tropical animal health and production》2014,46(2):399-404

The objectives of this study were to (1) investigate the occurrence of hepatitis E virus (HEV) in pigs from large-scale and family-scale farms, (2) genetically characterize the strains isolated, and (3) study the pathogenesis of swine HEV infection via immunohistochemistry. A total of 50 pigs from 10 farms in Mato Grosso State, Brazil were divided according to type of production system into either large-scale farms (n?=?5) or family-scale farms (n?=?5). Samples of liver, gallbladder, small and large intestines, bile, and feces from the pigs were analyzed by nested PCR with primers targeting the ORF2 region of HEV and by immunohistochemistry. Of the eight HEV-positive samples from pigs of family-scale farms, phylogenetic analysis revealed that seven of the swine HEV isolates clustered with subtype 3b of genotype 3 and one isolate was categorized with subtype 3 f. The HEV antigen was detected mainly in the small intestine samples from family-scale farms, suggesting an early stage HEV infection. HEV was not detected in the samples of pigs from large-scale farms, reinforcing the need for additional studies to evaluate the risk of transmission of HEV to humans from pigs from family-scale farms in Mato Grosso State.  相似文献