Pituitary responsiveness following infusion or repeated injections of [D-Leu6, des Gly-NH210]-LHRH-ethylamide in prepubertal ewes.     

M Q Mayar  G K Tarnavsky  J J Reeves 《Journal of animal science》1978,47(1):235-240

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Disappearance of LHRH and D-Leu-des Gly NH2 LHRH ethylamide in ewes.     

J A Dias  J J Reeves 《Journal of animal science》1978,46(6):1707-1711

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Scanning electron microscope study of Sendai virus fusion between HeLa cells and chick erythrocytes.     

A D Irvin  H S Anger  E R Young  J F Reeves 《Research in veterinary science》1978,25(1):67-75

Chick erythrocytes were fused with HeLa cells by Sendai virus and preparations examined by scanning electron microscopy at different times after fusion. Heterokaryons were usually formed by fusion of erythrocyte ghosts with HeLa cells. Occasionally whole erythrocytes were engulfed but there was no evidence that free nuclei fused. Initial inter-cell attachments were usually, and possibly always, made at the site of an attached virus particle. This study helps to correlate topographical findings with previous two-dimensional studies with the transmission electron microscope and may also provide a model system for the fusion of parasitised erythrocytes with eukaryotic cells.  相似文献   

Within-farm variability in number of females mated per week during a one-year period and breeding herd productivity on swine farms     

Koketsu Y  Duangkaew C  Dial GD  Reeves DE 《Journal of the American Veterinary Medical Association》1999,214(4):520-524

OBJECTIVE: To determine the effect of within-farm variability in the number of females mated per week during a 1-year period on annual breeding herd productivity in swine breeding herds and to apply statistical process control charts to measures of within-farm variability. DESIGN: Longitudinal study. SAMPLE POPULATION: 84 swine farms with a female inventory of 390 to 1,491 sows and gilts (mean, 761 females). PROCEDURE: As a measure of within-farm variability in a breeding herd, SD for the number of females mated per week during a 1-year period was evaluated. Two types of production records for 84 farms were evaluated. One file contained within-farm variability (SD) in mated females for each farm, and the second included annual productivity 19 weeks after week of mating. We also defined forewarning limits as mean +/- 2 SD, using a statistical process control chart. RESULTS: Larger within-farm variability in number of mated females was associated with lower annual measurements, such as fewer pigs weaned per mated female per year and lower farrowing rate. In addition, farms that did not have any weeks outside the forewarning limits for number of mated females produced more pigs weaned per mated female per year than those with 1 or more weeks of over- or underproduction. Furthermore, the number of weeks outside of forewarning limits was positively associated with within-farm variability in number of mated females. CLINICAL IMPLICATIONS: We recommend that farm managers determine a target range for the number of females mated per week to prevent large week-to-week variations in breeding herd operations.  相似文献   

Technical note: recombinant LHRH fusion protein suppresses estrus in heifers     

Sosa JM  Zhang Y  de Avila DM  Bertrand KP  Reeves JJ 《Journal of animal science》2000,78(5):1310-1312

A recombinant luteinizing hormone-releasing hormone (LHRH) fusion protein was evaluated for its effectiveness in suppression of estrus in heifers. Eight heifers were randomly assigned to two equal treatment groups. Treatments consisted of recombinant ovalbumin-LHRH-7 or recombinant ovalbumin (control). This recombinant chimeric fusion protein consisted of ovalbumin with seven LHRH peptides (ovalbumin-LHRH-7). The plasmid for this protein was expressed in E. coli and was collected and purified as an insoluble protein. One milligram of the respective proteins was suspended in 2 mL of Z-Max adjuvant and administered by intramammary injection three times at 7-wk intervals. Luteinizing hormone-releasing hormone antibody binding was elevated in heifers treated with ovalbumin-LHRH-7 compared to ovalbumin-treated heifers (P < .05). Serum progesterone concentrations (< 1 ng/mL) indicate that the estrous cycle of the four heifers treated with ovalbumin-LHRH-7 was suppressed for a time period ranging from 60 to 238 d, which was different from control heifers (P < .01). Serum progesterone for the control heifers continued to exhibit cyclic profiles over the experimental period. This preliminary study in heifers demonstrated that a chimeric LHRH fusion protein induced elevated concentrations of circulating LHRH antibodies that suppressed estrus for an average of 122 +/- 41 d.  相似文献   

Active immunization of beef heifers against luteinizing hormone: I. Evaluation of protein carriers and adjuvants on antigenicity of LH     

A J Roberts  D M deAvila  J D Gerber  J J Reeves 《Journal of animal science》1990,68(11):3742-3746

Two experiments were conducted to evaluate two carrier proteins and nine adjuvants in promoting antibody production in heifers immunized against LH. The anti-LH antibody response was evaluated in heifers immunized against LH conjugated to either ovalbumin or keyhole limpet hemocyanin (KLH) (Exp. 1). In Exp. 2, an LH-ovalbumin conjugate was used to evaluate effectiveness of nine different adjuvants in antibody production. Weekly blood samples were collected from all heifers throughout the 23-wk study to determine LH antibody binding activity. In Exp. 1, heifers immunized with the LH-ovalbumin (LH-oval) conjugate had greater LH antibody binding activities (P less than .001) than those immunized with the LH-KLH conjugate. In Exp. 2, nine groups of heifers were immunized with LH-oval suspended in one of nine adjuvants; a 10th group was immunized against ovalbumin alone (control). Only adjuvants that contained at least 40% oil resulted in LH antibody binding activity that differed (P less than .01) from control. These results show that ovalbumin was a superior carrier protein to KLH in enhancing antibody production; adjuvants with greater than 50% oil were superior to those with less oil in promoting LH antibody production.  相似文献   

A multivariable prognostic model for equine colic patients     

Mathew J. Reeves  Charles R. Curtis  Mo D. Salman  John S. Reif  Ted S. Stashak 《Preventive veterinary medicine》1990,9(4):241-257

A survey of 1965 equine colic cases was conducted from August 1985 to July 1986 ten equine referral hospitals located througout the U.S.A. Two-thirds of the cases were randomly selected for model development (1336), while the remaining cases (629) were used only for subsequent validation of the model. The following outcomes were defined: (1) died or killed prior to discharge from the hospital; or (2) alive at the time of discharge. Only variables which were significant (P<0.05) in an initial bivariate screening procedure and for which there were less than 400 missing values were considered in the multivariable modelling. A multivariable logistic regression model was constructed using a stepwise algorithm. The model used 666 cases and included the following variables: peripheral pulse (normal or weak), pulse rate, surgical or medical treatment, packed cell volume, self-inflicted trauma (absent or present) and capillary refill time. For each horse in the validation data set which had the appropriate variables recorded (n=335), the estimated expected probability of death (expected value) was calculated using the logistic regression equation. Using Bayes theorem, the post-test probability was calculated from the expected value (an estimate of the test odds) and the present probability (the case-fatality rate at each institution). Nomograms of predictive values for different case-fatality rates and expected values were constructed. Hosmer-Lemeshow goodness-of-fit statistics indicated that the model fitted the model data set well but the validation set poorly. However, when the observed case-fatality rates were compared with the average post-test probabilities for 0.10 increments of post-test probability, qualitatively, the model's performance was better.  相似文献   

商陆(Phytolacca acinosa Roxb.)的锰耐性和超积累   总被引：20，自引：0，他引：20  

薛生国  陈英旭  骆永明  Roger D Reeves  林琦 《土壤学报》2004,41(6):889-895

超积累植物的确证对成功实施重金属污染环境植物修复是必不可少的。通过野外调查和营养液培养试验 ,研究超积累植物商陆 (PhytolaccaacinosaRoxb )的锰富集特性 ,结果表明 ,商陆对生长介质中的Mn具有很强的耐性和累积能力。商陆在锰含量高达 114× 10 3 mgkg-1的尾矿废弃地上依然生长良好 ,叶锰含量最高达 19 3× 10 3 mgkg-1。温室培养条件下 ,当生长介质中Mn浓度为 8 0 0 0mmolL-1时 ,虽然其生物量与对照相比有所降低 ,但植株仍能生长。随着生长介质中Mn浓度的升高 ,商陆叶和茎的Mn含量逐渐增加 ,生物富集系数则逐渐降低 ,但是地上部分锰积累量则先增加后减少。当Mn浓度为 5 0 0 0mmolL-1时 ,商陆地上部分锰积累量达到最大值 2 5 8 2mgplant-1;当Mn浓度为 12 0 0 0mmolL-1时 ,商陆仍能完成整个生命周期 ,叶锰含量达到最大值 36 4× 10 3 mgkg-1,生物富集系数为 5 5。不同锰供应水平下 ,商陆吸收的锰有87%～ 95 %被转移到地上部分。这进一步验证商陆的锰超积累特性 ,为利用超积累植物对大面积污染土壤实施植物修复提供了有力证据 ,对锰污染土壤和水体实施植物修复具有很大的应用前景  相似文献   

Nitrogen mineralization potential of dairy manures and its relationship to composition   总被引：2，自引：0，他引：2  

J. Van Kessel  J. Reeves 《Biology and Fertility of Soils》2002,36(2):118-123

The objectives of this study were to determine the variability in mineralization of dairy manure N, to determine if N mineralization can be predicted by compositional factors or by near- or mid-infrared reflectance spectroscopy. Dairy manures (n =107) were collected from farms in Maryland, Virginia, Pennsylvania, New York, and Connecticut. The composition of these manures ranged from 14 to 386 g dry matter kg^-1, 0.9 to 9.5 kg total N/m³, and 0.3 to 4.7 kg NH₄⁺-N/m³. Manure-amended soil was aerobically incubated at 25°C and concentrations of NH₄⁺-N and NO₃^--N were determined at day 2 and day 56. The manures were highly variable in their N mineralization characteristics, ranging from a net mineralization of 54.9% to a net immobilization of 29.2% of the organic N. When compositional parameters were individually regressed against percentage mineralized organic N, the highest correlation coefficient (r) was 0.164. A stepwise regression of all 11 variables yielded a maximal r of 0.486. These results suggest that the availability of dairy manure organic N is highly variable and that the availability cannot be predicted from simple compositional parameters. No relationship was found between near-infrared spectral characteristics and N mineralization suggesting that no simple relationship exists between N mineralization and compositional characteristics. There appears to be some potential for the use of mid-infrared for determining the mineralization potential of manures.  相似文献   

In-row tillage methods for subsoil amendment and starter fertilizer application to conservation-tilled grain sorghum     

D.W. Reeves  J.H. Edwards  C.B. Elkins  J.T. Touchton 《Soil & Tillage Research》1990,16(4):359-369

Acid subsoils and tillage pans limit crop yields on sandy soils of the Southern Coastal Plain of the United States. Studies were conducted for 3 years on two soils with acid subsoils and tillage pans to determine the effect of starter fertilizer (22 kg N, 10 kg P ha⁻¹ and fluid lime (1350 kg ha⁻¹) placement with in-row tillage methods on growth and yield of grain sorghum (Sorghum bicolor (L.) Moench) grown in a conservation-tillage system. Fertilizer and lime were applied in factorial combinations in the in-row subsoil channel, in a narrow (4-mm) slit 18 cm below the tillage pan (slit-tillage), or 7 cm to the side of the row incorporated 7 cm deep. Slit-tillage was as effective as subsoiling in two of the four tests where plant growth and grain yield responded to deep tillage. Of the other two tests where there was a response to deep tillage, slit-tillage resulted in a 6% decrease in grain yield compared to subsoiling in one test, and an 8% yield increase in the other. Starter fertilizer placement was not critical, but response to starter fertilizer occurred only when deep tillage, either in-row subsoiling or slit-tillage, was used in conjunction with the fertilizer. Starter fertilizer consistently increased early-season plant growth; however, yield response to starter fertilizer was highly dependent on rainfall. Starter fertilizer application increased yield in only one of five tests. There was no benefit from injecting lime.  相似文献