Glucuronic acid metabolism in dairy cows. Excretion in the urine     

J Ushijima  K Koyama  A Yuasa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1964,26(1):43-51

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Persistent infection with chicken anaemia virus and some effects of highly virulent infectious bursal disease virus infection on its persistency     

Imai K  Mase M  Tsukamoto K  Hihara H  Yuasa N 《Research in veterinary science》1999,67(3):233-238

Chicken anaemia virus (CAV) infectivity and the effect of highly virulent infectious bursal disease virus (hv IBDV) infection on CAV's infectivity were examined in chickens inoculated with CAV or inoculated dually with CAV and hv IBDV. Five chickens inoculated dually with hv IBDV at 35 days old and then with CAV at 40 days old exhibited no clinical signs of disease, but showed atrophic bursae of Fabricius when necropsied 4 weeks later. Upon examining the chickens at 7 days postinoculation (dpi) with CAV, it was found that hv IBDV infection had inhibited production of virus neutralising (VN) antibody to CAV, and that it was possible to recover CAV from plasma of these chickens. Although VN antibody to CAV appeared after 14 dpi, CAV was recovered from blood cells (BC s) at high titres ranging from 10(2.5)to 10(5.5)TCID(50)/0.1 ml, 7 to 28 dpi in IBDV -induced immunosuppressed chickens. In addition, CAV was sporadically recovered, using rectal swabs, from the dually inoculated chickens at low titers, ranging from 10(1.0)to 10(2. 0)TCID(50)/0.1 ml). In contrast, although CAV was recovered from BC s in most of the chickens inoculated with CAV alone, the titers were lower (10(1.0)to 10(2.5)TCID(50)/0.1 ml). No CAV was detected from the rectal swabs of these chickens. The results of virus recovery were confirmed by polymerase chain reaction. This study first examined the persistency of CAV in BC s and the effective enhancement of primary CAV infection as a result of immunosuppression caused by hv IBDV infection.  相似文献   

Recognition pattern of thyroglobulin autoantibody from hypothyroid dogs to tryptic peptides of canine thyroglobulin     

Tani H  Shimizu R  Sasai K  Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1049-1056

Circulating thyroglobulin autoantibody (TgAA) was analyzed using the Western immunoblot for determination of the dominant epitopes recognized by TgAA on tryptic peptides of canine thyroglobulin (cTg) in hypothyroid dogs. TgAA was measured in hypothyroid dogs, non-hypothyroid dogs with skin diseases and clinically normal dogs. Five of the 7 hypothyroid dogs, 1 of the 8 dogs with skin diseases and 1 of the 4 normal dogs were positive for TgAA. Four of the 5 TgAA-positive hypothyroid dogs were Golden Retrievers, and 3 of them showed high antibody titers. The sera of TgAA positive-dogs reacted to several peptides, and their patterns varied from sample to sample. Sera from 3 dogs with high titers of TgAA reacted broadly to high molecular weight peptides ranging from 45 to 90 kDa. These Western immunoblot patterns of the sera were disappeared after pretreatment with sufficient amount of intact cTg. All serum samples of both TgAA positive dogs and negative controls reacted to low molecular weight peptides ranging from 15 to 20 kDa. These immunoblot patterns of the sera were not disappeared even after pretreatment with sufficient amount of intact cTg. These findings show the possibility that the epitopes recognized by TgAA depend upon individual dogs with hypothyroidism and these autoantibodies recognize conformational epitopes on the cTg molecule.  相似文献   

Effects of acrosomal conditions of frozen-thawed spermatozoa on the results of artificial insemination in Japanese Black cattle     

Kazumi KISHIDA  Mitsuhiro SAKASE  Kenta MINAMI  Miyuki M. ARAI  Reiko SYOJI  Namiko KOHAMA  Takayuki AKIYAMA  Akio OKA  Hiroshi HARAYAMA  Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524

The purposes of this study were to examine the relationship between male artificial insemination (AI) fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4) in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques were significantly correlated with the conception rates of routine AI, rates of transferable embryos in superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with oocytes.  相似文献   

Seedling survival and growth during the 2 years following seed germination of Magnolia stellata,a threatened subcanopy tree,after clearcutting     

Ichiro Tamaki  Katsushige Nomura  Reiko Nomura  Chieko Tate  Mika Ogiso  Yoshihiro Miyakami  Yumiko Yabe 《Journal of Forest Research》2015,20(4):415-419

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A Study on Borna Disease Virus Infection in Domestic Cats in Japan     

Azusa SOMEYA  Ryoko FUKUSHIMA  Michiko YOSHIDA  Yasuyuki TANAHASHI  Tangmunkhong PRAPEUK  Reiko IIZUKA  Hiroshi HIRAMI  Atsushi MATSUDA  Shunichi TAKAHASHI  Goro KURITA  Takashi KIMURA  Misuzu SEO  Masayuki FUNABA  Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160

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Validity of data filtering technique for the behavior analysis of crab in neritic and closed sea by ultrasonic biotelemetry     

Yoshinori Miyamoto  Keiichi Uchida  Reiko Orii  Toshiharu Kakihara  Masahiko Furusawa 《Fisheries Science》2006,72(1):211-213

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Granulomatous Pododermatitis in the Digits Caused by Fusarium proliferatum in a Cat     

Go SUGAHARA  Akio KIUCHI  Reiko USUI  Ryouichi USUI  Takayuki MINESHIGE  Junichi KAMIIE  Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):435-438

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Immune response of humpback grouper, Cromileptes altivelis (Valenciennes) injected with the recombinant coat protein of betanodavirus   总被引：4，自引：0，他引：4  

K Yuasa  I Koesharyani  D Roza  K Mori  M Katata  & T Nakai 《Journal of fish diseases》2002,25(1):53-56

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Differential detection of shrimp and crab for food labeling using polymerase chain reaction     

Taguchi H  Watanabe S  Temmei Y  Hirao T  Akiyama H  Sakai S  Adachi R  Sakata K  Urisu A  Teshima R 《Journal of agricultural and food chemistry》2011,59(8):3510-3519

Shrimp and crab are well-known as allergenic ingredients. According to Japanese food allergy labeling regulations, shrimp species (including prawns, crayfishes, and lobsters) and crab species must be differentially declared when ≥10 ppm (total protein) of an allergenic ingredient is present. However, the commercial ELISA tests for the detection of crustacean proteins cannot differentiate between shrimp and crab. Therefore, two methods were developed to discriminate shrimp and crab: a shrimp-PCR method with postamplification digestion and a crab-PCR method that specifically amplifies a fragment of the 16S rRNA gene. The sensitivity and specificity of both PCR methods were verified by experiments using DNA extracted from 15 shrimp species, 13 crab species, krill, mysid, mantis shrimp, other food samples (cephalopod, shellfish, and fish), incurred foods, and commercial food products. Both PCR methods could detect 5 pg of DNA extracted from target species and 50 ng of genomic DNA extracted from incurred foods containing 10 ppm (μg/g) total protein of shrimp or crab. The two PCR methods were considered to be specific enough to separately detect species belonging to shrimp and crab. Although false-positive and false-negative results were obtained from some nontarget crustacean species, the proposed PCR methods, when used in conjunction with ELISA tests, would be a useful tool for confirmation of the validity of food allergy labeling and management of processed food safety for allergic patients.  相似文献