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1.
A method for screening wood extractives was developed using cellulose thin-layer chromatography plate (Cell-TLC) separation and was directly applied to bioassays. Wood meal (60 mesh) from nangka (Artocarpus heterophyllus Lamk) heartwood was extracted with hot methanol. The crude extract was separated using a Cell-TLC plate (50 × 50mm). Two broad bands with Rf values of 0.46 and 0.96 were found, and the bands showed completely different effects against the pest termite Coptotermes formosanus Shiraki and the decay fungus Fomitopsis palustris. The band with Rf 0.46 was preferentially consumed by workers of C. formosanus, and it did not show any growth inhibition against F. palustris when the Cell-TLC plate was directly exposed to the organism. In contrast, the band with Rf 0.96 appeared to repel strongly the feeding by C. formosanus and inhibited the growth of F. palustris. It was concluded that the Cell-TLC system was applicable for screening wood extracts consisting of many compounds.  相似文献   
2.
Discovery of new alleles at important gene loci through allele mining could support the rice improvement program to sustain national food security. Evaluation of the existing Indonesian local rice landraces is an important point to detect the potential of functional alleles. One of the bacterial leaf blight (BLB) resistance alleles, Xa7 was detected in Indonesian rice landrace germplasm, Parekaligolara. To validate this potential allele, field evaluation on the segregating population, expression analysis using real time RT-PCR, and sequencing were carried out. Two selected Parekaligolara progenies lines (F4 and F6) from double crosses with other selected landraces were clearly more resistant to a dominant Indonesian BLB, Race IV. Specific primers of Xa7-LD40 successfully amplified the alleles of F4 and F6 lines approximately 300 bp in length. The amplicon sequenced using vector-targeted primers, resulting 264 bp which were flanked between 602 and 866 bp sites. The translated sequence which produced 60 amino acids (open reading frame) ORF, showed homology with the encoding gene associated with the defense system to biotic stress, BTB/POZ. As integrated researches for many potential biotic and abiotic stresses alleles on Indonesian landraces germplasm, this outcome expectedly supports rice landraces utilization for developing of elite cultivars which survive on global changed conditions and benefiting to national food security.  相似文献   
3.
A new social forestry program has been implemented in Java to overcome encroachment of state forests. In this program, the state and local communities jointly manage the state forests and share the benefits of increased forest resource stock and flow as a result of the management. This article aims to investigate the complete incentive structure of the social forestry program and how the incentive structure changes community member participation in forest management. Property rights transfers and economic analyses were employed to analyze the incentive structure. We conclude that although incomplete, the transfer of rights from the state to the community members is a useful approach toward establishing an effective incentive structure and provides a net gain to the community members. The extent of community participation, however, depends not only on the incentive structure but also on the social capital that exists in the community.  相似文献   
4.
The effectiveness of green mussel (Perna viridis) cultivation was compared between longline culture and the traditional bamboo stake method using different mussel densities in a rural area on the north coast of Java, Indonesia. The study took place in a rural area about 2 km north‐east from the city of Semarang where unsustainable shrimp and fish pond culture in the past has led to massive mangrove loss, spiralling environmental degradation and socio‐economic disintegration. Mangrove‐friendly alternatives for a sustainable socio‐economic recovery of local livelihoods are urgently being sought. Longlines were more successful than the stake method in spat collection. Longlines also showed a small but significantly higher relative weight gain and specific growth rate for mussels than bamboo stakes. Mussels in lower densities showed higher survival and grew to larger individual sizes, but initial seeding density had no significant effect on relative weight gain or specific growth rate per stocking. Slightly lower set‐up costs and time investment and somewhat higher yields for longlines give an almost twofold higher income per time unit of own time invested by the farmer for longline culture over the traditional stake culture method. The profitability of mussel culture using the stake method is below the average hourly wage for skilled labour, but above that when using the longline method. We conclude that green mussel culture using the longline system is feasible as an alternative to less sustainable forms of livelihood for the local communities in mangrove areas.  相似文献   
5.
This study was carried out to clarify the role of lymphocyte subpopulations and Babesia-specific antibody on the treatment of clindamycin in dogs infected with B. gibsoni. Ten beagle dogs were divided into two groups: an untreated group (5 dogs) and a clindamycin-treated group (5 dogs), which was administered clindamycin at 25 mg/ kg body weight, per os, q 12 hr from 7 days to 21 days post-infection (PI). On the acute stage of infection, clindamycin treatment resolved anaemia and other clinical findings. There were no significant differences between treated and untreated dogs either in parasitemia levels or Babesial IgG antibody levels. However, morphological changes that indicated degeneration in the majority of parasites were observed. The numbers of CD4(+) showed a significant increase in treated dogs, especially after treatment. On the chronic stage, CD4(+) cells maintained high level both of the treated and untreated dogs. Although parasitemia maintained low level, their relapses were occurred on the 49th day PI in treated dogs and on the 42nd and 63rd PI in untreated dogs. A rapid humoral antibody response was observed in treated dogs, however, lower humoral antibody responses in untreated dogs after relapses. The antibody levels of treated dogs were significantly higher than those of untreated dogs. These results suggested that clindamycin might not eliminate rapidly parasites from peripheral blood, but damage parasites, which might stimulate efficiently humoral and cellular immunity against Babesia infection, and result in an improvement of clinical conditions.  相似文献   
6.
Three cultivars of snake fruits, Pondoh Hitam, Pondoh Super, and Gading, were freshly extracted using liquid-liquid extraction. The aroma compounds of the three samples were analyzed by GC-MS and GC-olfactometry using the nasal impact frequency (NIF) method. A total of 24 odor-active compounds were associated with the aroma of snake fruit. Methyl 3-methylpentanoate was regarded as the character impact odorant of typical snake fruit aroma. 2-Methylbutanoic acid, 3-methylpentanoic acid, and an unknown odorant with very high intensity were found to be responsible for the snake fruit's sweaty odor. Other odorants including methyl 3-methyl-2-butenoate (overripe fruity, ethereal), methyl 3-methyl-2-pentenoate (ethereal, strong green, woody), and 2,5-dimethyl-4-hydroxy-3[2]-furanone (caramel, sweet, cotton candy-like) contribute to the overall aroma of snake fruit. Methyl dihydrojasmonate and isoeugenol, which also have odor impact, were identified for the first time as snake fruit volatiles. The main differences between the aroma of Pondoh and Gading cultivars could be attributed to the olfactory attributes (metallic, chemical, rubbery, strong green, and woody), which were perceived by most of the panelists in the Pondoh samples but were not detected in the Gading samples. This work is a prerequisite for effective selection of salak genotypes with optimal aroma profiles for high consumer acceptance.  相似文献   
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8.
Rice eating quality is considered to be one of the top priorities in determining the agronomical value of rice; thus, the rapid evaluation of eating quality at early breeding generations in breeding programmes for better eating quality is of great importance. In an attempt to develop DNA markers associated with eating quality of indica rice, we used multiple regression analysis to test 54 markers, which were preselected for their possible association with eating quality, using 24 indica varieties with different palatability scores. Of these markers, eighteen markers were found to be significantly associated with palatability according to sensory evaluation. Accordingly, a marker set in the model regression equation with a high R2 (0.997) was formulated to estimate indica rice palatability. Validation suggests that markers and the statistical parameters formulated by the equation could be a potential tool to predict the palatability of cooked Indonesian indica rice and could be reliable in developing country‐dependent model equations for eating quality.  相似文献   
9.
A genetic analysis of blast resistance in upland rice variety is very crucial. In this study, we performed a linkage mapping of quantitative trait loci (QTLs) for blast resistance using an advanced backcross population from a cross between Way Rarem (susceptible indica variety) and Oryzica Llanos 5 (durable resistant indica variety). A transgressive segregation was observed in the advanced backcross population of Way Rarem//Oryzica Llanos 5. A total of 16 QTLs have been identified along chromosomes 1, 3, 5, 6, 7, 8, 9, and 11 against eight blast pathogen isolates. Each QTL accounted from 11.31 to 45.11% of the variation in blast resistance. Most QTLs showed race specificity, demonstrating the small effect of such QTLs. Unexpectedly, several superior blast resistance alleles were contributed by Way Rarem, the susceptible-recurrent parent. Among eight candidate defense response genes detected in several loci, a single gene (oxalate oxidase) present on chromosome 3 was found to be associated with blast resistance in upland indica rice. Ultimately, these advanced backcross lines with resistance to blast tagged by markers might be useful for pyramiding blast resistance alleles in upland rice.  相似文献   
10.
This report examines the effectiveness of clindamycin for the treatment of babesiosis in dogs (n=10) experimentally infected with Babesia gibsoni (B. gibsoni). Clindamycin (25 mg/kg body weight, per os, q 12 hours for 14 days) gradually reduced parasitemia levels and induced morphological changes that indicated degeneration of parasites (e.g., segmentation; size reduction; localization in the cell limbic and/or torn state of the nucleus; and swelling, decrease, or disappearance of the cytoplasm) in the majority of dogs. Clindamycin treatment reduced the clinical symptoms characteristic of Babesia infection, including anemia, anorexia, and listlessness. Clindamycin might be useful as a medicine for treatment of B. gibsoni infection.  相似文献   
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