Effects of green tea polyphenol (-)-epigallocatechin-3-gallate on newly developed high-fat/Western-style diet-induced obesity and metabolic syndrome in mice     

Chen YK  Cheung C  Reuhl KR  Liu AB  Lee MJ  Lu YP  Yang CS 《Journal of agricultural and food chemistry》2011,59(21):11862-11871

The aim of this study was to investigate the effects of (-)-epigallocatechin-3-gallate (EGCG) on newly developed high-fat/Western-style diet-induced obesity and symptoms of metabolic syndrome. Male C57BL/6J mice were fed a high fat/Western-style (HFW; 60% energy as fat and lower levels of calcium, vitamin D(3), folic acid, choline bitartrate, and fiber) or HFW with EGCG (HFWE; HFW with 0.32% EGCG) diet for 17 wks. As a comparison, two other groups of mice fed a low-fat diet (LF; 10% energy as fat) and high-fat diet (HF; 60% energy as fat) were also included. The HFW group developed more body weight gain and severe symptoms of metabolic syndrome than the HF group. The EGCG treatment significantly reduced body weight gain associated with increased fecal lipids and decreased blood glucose and alanine aminotransferase (ALT) levels compared to those of the HFW group. Fatty liver incidence, liver damage, and liver triglyceride levels were also decreased by the EGCG treatment. Moreover, the EGCG treatment attenuated insulin resistance and levels of plasma cholesterol, monocyte chemoattractant protein-1 (MCP-1), C-reactive protein (CRP), interlukin-6 (IL-6), and granulocyte colony-stimulating factor (G-CSF). Our results demonstrate that the HFW diet produces more severe symptoms of metabolic syndrome than the HF diet and that the EGCG treatment can alleviate these symptoms and body fat accumulation. The beneficial effects of EGCG are associated with decreased lipid absorption and reduced levels of inflammatory cytokines.  相似文献   

TOXICITY OF THE RADIOGRAPHIC CONTRAST MEDIA IOPAMIDOL, IOHEXOL AND METRIZAMIDE TO CELL CULTURES     

Carol H.  Carlisle FACVSc.  Michael A.  Pass  Ph.D.  Herb E.  Lowndes  Ph.D.  Kenneth R.  Reuhl  Ph.D. 《Veterinary radiology & ultrasound》1995,36(3):207-211

Cultured murine embryonal carcinoma cells were exposed to the tri-iodinated radiographic contrast media iopamidol, iohexol and metrizamide at concentrations below those used for clinical myelography and examined by light and electron microscopy. Cytologic changes consisting of swelling and vacuolation of mitochondria and other cytoplasmic organelles were observed within 1 h of exposure to the contrast media. By 12 h of incubation cells altered shape, lifted from the culture dish and eventually died. These changes occurred irrespective of the osmolarity of the incubation medium and did not occur when cells were incubated in the presence of 1.16 mM EDTA or 10 mM Tris, which are present in commercial preparations of iopamidol and iohexol. Similar cytological changes were observed in cultures of neurons derived from embryonal carcinoma cells and in cultures of rat dorsal root ganglion cells. The results indicate that iopamidol, iohexol and metrizamide are cytotoxic to cells in culture at less than 20% of the concentration used for myelography and this could contribute to the adverse reactions to myelography seen in people and animals.  相似文献