排序方式： 共有51条查询结果，搜索用时 15 毫秒
1.
Effect of intramammary injection of rboGM-CSF on milk levels of chemiluminescence activity, somatic cell count, and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis 
下载免费PDF全文
下载免费PDF全文 Hideyuki Takahashi Masaharu Odai Kenji Mitani Shigeki Inumaru Shozo Arai Rieko Horino Yuichi Yokomizo 《Canadian journal of veterinary research》2004,68(3):182-187
The effect of intramammary injection of recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF, 400 microg/10 mL) on quarter milk levels of chemiluminescence (CL) activity, and somatic cell count (SCC) and shedding pattern of Staphylococcus aureus was investigated. Ten Holstein cows, naturally infected with S. aureus were used, with either early-stage or late-stage subclinical mastitis. Injection of rboGM-CSF caused a remarkable increase in milk CL activity with a peak at 6 h after the cytokine injection in the early- and late-stage groups. In the early-stage group, milk SCC stayed around preinjection level at 6 h, rose significantly on days 1 and 2, and was followed by a smooth and significant decline to an under preinjection level (below 200 000 cells/mL) on day 7 postinjection. Alternatively, in the late-stage group, milk SCC rose significantly at 6 h after the cytokine injection and maintained high levels thereafter. The milk S. aureus count decreased drastically by the cytokine injection in the early-stage group. The bacterial count was moderately decreased in the late-stage group, but increased back to preinoculation levels on day 7 after the cytokine injection. The results suggest that the rboGM-CSF has a potential as a therapeutic agent for S. aureus infection causing subclinical mastitis of dairy cows, if the cytokine is applied at the initial stage of infection. 相似文献
2.
Rieko OKAME Keiko NAKAHARA Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):815-821
We measured the plasma levels of amino acids at various reproductive stages in female
rats, including the estrous cycle, pregnancy and lactation, and compared the resulting
amino acid profiles using two- or three-dimensional figures. These figures revealed that
the amino acid profiles of pregnant and lactating dams differed considerably from those
during the estrous cycle or in male rats. The plasma levels of individual amino acids were
almost the same between proestrus, estrus, metestrus and diestrus, and their profiles did
not differ significantly. However, the amino acid profiles changed during pregnancy and
lactation in dams. The plasma Ser level decreased significantly in mid and late pregnancy,
whereas Tyr, Gly and His decreased significantly in the late and end stages of pregnancy,
and Trp and Lys significantly decreased and increased at the end of pregnancy,
respectively. Much larger changes in amino acid profiles were observed during lactation,
when the levels of many amino acids increased significantly, and none showed a significant
decrease. Plasma Pro, Ser and Gly levels increased continuously from day 1 until day 15 of
lactation, whereas Asn and Met increased significantly from days 1 and 5 respectively
until the end of lactation. These results suggest that the profiles of plasma amino acids
show characteristic changes according to reproductive stage and that it may be necessary
to consider such differences when performing amino acid-based diagnosis. 相似文献
3.
Taguchi H Watanabe S Hirao T Akiyama H Sakai S Watanabe T Matsuda R Urisu A Maitani T 《Journal of agricultural and food chemistry》2007,55(5):1649-1655
Kiwifruit (Actinidia deliciosa and Actinidia chinensis) is allergenic to sensitive patients, and, under Japanese regulations, it is one of the food items that are recommended to be declared on food labeling as much as possible. To develop PCR-based methods for the detection of trace amounts of kiwifruit in foods, two primer pairs targeting the ITS-1 region of the Actinidia spp. were designed using PCR simulation software. On the basis of the known distribution of a major kiwifruit allergen (actinidin) within the Actinidia spp., as well as of reports on clinical and immunological cross-reactivities, one of the primer pairs was designed to detect all Actinidia spp. and the other to detect commercially grown Actinidia spp. (i.e., kiwifruit, Actinidia arguta, and their interspecific hybrids) except for Actinidia polygama. The specificity of the developed methods using the designed primer pairs was verified by performing PCR experiments on 8 Actinidia spp. and 26 other plants including fruits. The methods were considered to be specific enough to yield target-size products only from the target Actinidia spp. and to detect no target-size products from nontarget species. The methods were sensitive enough to detect 5-50 fg of Actinidia spp. DNA spiked in 50 ng of salmon testis DNA used as a carrier (1-10 ppm of kiwifruit DNA) and 1700 ppm (w/w) of fresh kiwifruit puree spiked in a commercial plain yogurt (corresponding to ca. 10 ppm of kiwifruit protein). These methods would be expected to be useful in the detection of hidden kiwifruit and its related species in processed foods. 相似文献
4.
Masaaki Katayama Yasuhiko Okamura Shunsuke Shimamura Rieko Katayama Hiroaki Kamishina Yuji Uzuka 《Research in veterinary science》2014
Graft perfusion with cold heparinized saline has known to induce ischemia and reperfusion injury in feline kidney transplantation. In this study, the effects of phosphate-buffered sucrose solution and heparinized saline solution on early kidney graft function were compared in feline kidney autotransplantation. Perfusion of grafts with or without hypothermic storage with chilled phosphate-buffered sucrose solution prevented ischemia and reperfusion injury despite a very short ischemic time. The results of our study suggest that phosphate-buffered sucrose perfusion and storage solution should be effective to reduce ischemia and reperfusion injury despite a very short ischemic time in feline kidney transplantation. 相似文献
5.
Masaaki Katayama Yasuhiko Okamura Rieko Katayama Jun Sasaki Shunsuke Shimamura Yuji Uzuka Hiroaki Kamishina Yoshinori Nezu 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(4):381-386
A 12-year-old, 3.5-kg spayed female domestic shorthair cat had a tracheal mass identified as malignant B-cell lymphoma. The cat had tracheal resection and subsequently developed laryngeal paralysis. Due to multiple episodes of respiratory distress the cat subsequently had tracheal surgeries. Finally, the cat had a sudden onset of severe respiratory distress and collapsed. Computed tomography imaging and arterial blood gas analysis supported a diagnosis of acute lung injury. 相似文献
6.
T Itabisashi R Horino K Hirano M Maeda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(3):551-558
Nine Suffolk sheep and 4 Holstein cattle were employed to observe electroencephalographic changes in experimental cerebrocortical necrosis. Amprolium (600 mg/kg/day) alone was given intraruminally 6 sheep and 4 cattle, all of which showed neurological signs and abnormal electroencephalograms. Both amprolium and thiamine (200 mg/day) and thiamine (50 mg/day) alone were given 2 and 1 sheep, respectively, as controls, which did not show any abnormal signs. Abnormal electroencephalograms included continuous slow waves and long-lasting spindles, both of which appeared diffusely and were seen subclinically or with neurological signs. The spindles consisted of slow waves or sharp- (or spike-) and-slow-wave complexes with or without convulsive seizures even during the convulsion stage. Conversely, the seizures occurred with the spindles or slow waves. 相似文献
7.
Chhourn Orn Rieko Shishido Masahiro Akimoto Ryo Ishikawa Than Myint Htun Ken-Ichi Nonomura Yohei Koide Men Sarom Seng Vang Sakhan Sophany Ouk Makara Takashige Ishii 《Breeding Science》2015,65(5):430-437
A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources. 相似文献
8.
Ingestion of water-soluble soybean fiber prevents osteopenia and hypercholesterolemia induced by ovariectomy in rats 总被引：2，自引：0，他引：2
Mitamura R Hara H Aoyama Y Takahashi T Furuta H 《Journal of agricultural and food chemistry》2003,51(4):1085-1089
This study examined the effects of feeding water-soluble soybean fiber (WSSF), a highly fermentable dietary fiber with low viscosity, on intestinal calcium (Ca) absorption, bone mineral content, and serum total cholesterol levels using female Sprague-Dawley rats (5 weeks old) with or without ovariectomy (OVX). The rats in each group were fed a WSSF (50 g/kg of diet) or WSSF-free diet for 4 weeks. Ca absorption was higher in OVX rats fed the WSSF diet than in OVX rats fed the WSSF-free diet. Femoral Ca content in both sham and OVX rats fed the WSSF diet was higher than that in rats fed the WSSF-free diet. The serum total cholesterol levels were elevated after OVX compared with that in the sham rats, and this elevation was prevented by the WSSF diet. In conclusion, ingestion of WSSF prevented osteopenia and hypercholesterolemia induced by ovariectomy in rats. 相似文献
9.
Koiwai M Hamaoka T Haritani M Shimizu S Zeniya Y Eto M Yokoyama R Tsutsui T Kimura K Yamane I 《Veterinary parasitology》2006,135(2):175-179
Serum samples from 2420 clinically healthy dairy cattle, randomly selected from stored sera in 18 districts of Japan, were tested for the presence of Neospora caninum antibodies using an indirect fluorescent antibody test (titer > or =1:200). Nationwide seroprevalence is estimated at 5.7% (139/2420). Seropositive cattle were detected in all surveyed districts despite the evidence of confirmed case reports of bovine neosporosis, showing that N. caninum is widely distributed throughout Japan. Age-specific seroprevalence did not increase with cattle age, suggesting that Neospora infection is likely to be transmitted vertically rather than horizontally in Japan. Considering that N. caninum seropositive cows are thought to be more likely to abort, substantial fetal losses may be induced by N. caninum infection in Japan. Devising strategies are needed to reduce the economic impact on the Japanese dairy industry. This is the first study to investigate the nationwide seroprevalence of N. caninum in cattle in Asia. 相似文献
10.
Takuo Hishi Rieko Urakawa Naoaki Tashiro Yuka Maeda Hideaki Shibata 《Biology and Fertility of Soils》2014,50(2):343-356
This study aimed to evaluate the spatial patterns of soil nitrogen (N) transformations in relation to slope aspect and position, and to investigate the main factors controlling N transformation patterns during both the growing and dormant seasons in cool-temperate deciduous natural forests and larch plantations in eastern Hokkaido, northern Japan. Net rates of N mineralization (NRminN) and of nitrification (NRnit) in surface soils on north-facing and lower slopes were higher than those on south-facing and upper slopes, whereas the net rate of ammonium-N production (NRamm) on south-facing and upper slopes was higher than that on north-facing slopes in both the natural forests and larch plantations. Both NRminN and NRnit were higher in the growing than in the dormant season, whereas NRamm was higher in the dormant season. The soil C/N ratio, water content, soil pH and frequency of freeze–thaw cycles were important variables affecting N transformation patterns in any season. In relation to seasonality, the solar radiation index, daily temperature range and earthworm biomass were important controlling factors only during the growing season, and watershed area and soil N concentration only during the dormant season, suggesting that biological control accompanied with wet–dry events were important factors affecting N transformations during the growing season, but that run-off water and chemical controls were important determinants of spatial variation in N transformations during the dormant season. 相似文献