Suppression of ovarian progesterone production in dairy cows using an implant of GnRH-agonist (deslorelin) for the purpose of evaluating progesterone metabolism     

AR RABIEE  KL MACMILLAN  F SCHWARZENBERGER  D THALLER  MJ RATHBONE  TE TRIGG 《Australian veterinary journal》2001,79(10):690-694

OBJECTIVE: To evaluate the potential of an implant of a GnRH-agonist (deslorelin) to create a progesterone free animal suitable for studying progesterone (P4) metabolism in intact cows by measuring blood P4 and faecal P4 metabolites. METHODS: Experiment 1: Eighteen non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to one of three groups to study plasma P4 concentrations preceding an intravaginal insert. These groups comprised: i) a deslorelin group (GnRH-agonist implanted); ii) a PGF group receiving two injections of prostaglandin (PGF2alpha) 12 days apart; and, iii) an ovariectomised (OVX) group. An intravaginal device (CIDR) was inserted into the vagina of each animal and left in place for 11 days. Plasma P4 concentrations were measured during the study period. Experiment 2: Twelve non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to two groups: i) a deslorelin group (GnRH-agonist implanted); and ii) an ovariectomised group. Plasma P4 and faecal P4 metabolites (20-oxo-pregnanes, 20alpha-OH and 20beta-OH) were monitored for a period of 5 weeks. RESULTS: Experiment 1: Average plasma P4 concentration did not differ between the three groups (1.28, 1.43 and 1.55 ng/mL for deslorelin, OVX and PGF cows, respectively, P = 0.8) during the period of supplementation. Experiment 2: There was no difference in plasma P4 (mean plasma P4 < 0.02 ng/mL, P = 0.9) and faecal P4 metabolites between deslorelin and OVX cows 2 weeks after the implantation (P = 0.7). CONCLUSIONS: These data showed that a GnRH-agonist (deslorelin) implant may be used as an alternative to ovariectomy to create a progesterone free animal suitable for studying the metabolism of administered P4.  相似文献   

Correlations between ultrasonography findings and hormonal profiles at oestrus in pure Spanish breed mares     

JC ILLERA  MJ ILLERA  G SIL VAN  M ILLERA 《Australian veterinary journal》1993,70(7):273-275

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An evaluation of a live Toxoplasma gondii vaccine in Tammar wallabies (Macropus eugenii)     

MJ LYNCH  DL OBENDORF  P STATHAM  GL REDDACLIFF 《Australian veterinary journal》1993,70(9):352-353

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Activated coagulation times of normal cows     

Riley JH  Lassen ED 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1979,8(1):31-33

The Activated Coagulation Time (ACT) was determined on 10 normal cows. Duplicate samples were taken from each cow. The mean ACT was 145 seconds with a range of 120-180 seconds. Standard deviations were 18 and 13 for the first and second sampling, respectively. Repeatability of the ACT was acceptable.  相似文献   

Primary tonsillar mast cell tumour in a dog          下载免费PDF全文

CC Shekell  MJ Thomson  RI Miller  JT Mackie 《Australian veterinary journal》2018,96(5):184-187

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Detection and genetic diversity of porcine rotavirus A,B and C in eastern Australian piggeries     

B Genz  J Gerszon  Y Pollock  B Gleeson  R Shankar  MJ Sellars  RJ Moser 《Australian veterinary journal》2023,101(4):153-163

Rotaviruses (RV) have a high prevalence in piggeries worldwide and are one of the major pathogens causing severe diarrhoea in young pigs. RV species A, B, and C have been linked to piglet diarrhoea in Australian pig herds, but their genetic diversity has not been studied in detail. Based on sequencing of the structural viral protein 7 (VP7) RVA G genotypes G3, G4 and G5, and RVC types G1, G3, G5, and G6 have been identified in Australian piggeries in previous studies. Although occurrence of RVB was reported in Australia in 1988, no further genetic analysis has been conducted. To improve health management decisions in Australian pig herds, more information on RV prevalence and genetic diversity is needed. Here, 243 enteric samples collected from 20 pig farms within Eastern Australia were analysed for the presence of RV in different age groups using a novel PCR-based multiplex assay (Pork MultiPath™ enteric panel). RVA, RVB, and RVC were detected in 10, 14, and 14 farms, respectively. Further sequencing of VP7 in selected RV-positive samples revealed G genotypes G2, G5, G9 (RVA), G6, G8, G14, G16, G20 (RVB), and G1, G3, G5, G6 (RVC) present. RVA was only detected in young (<10 weeks old) pigs whereas RVB and RVC were also detected in older animals (>11 weeks old). Interestingly, RVB and RVC G-type occurrence differed between age groups. In conclusion, this study provides new insights on the prevalence and diversity of different RV species in pig herds of Eastern Australia whilst demonstrating the ability of the Pork MultiPath™ technology to accurately differentiate between these RV species.  相似文献   

Level and Timing of Nitrogen Fertilizer Application to Early and Semi-early Potatoes (Solanum tuberosum L.) Grown with Irrigation on Light Soils in Norway     

Hugh Riley 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):122-134

Trials were performed with early and semi-early potatoes to test the effects of nitrogen (N) fertilizer level (0-160 kg N ha^-1) and timing (all at planting versus half then and half either soon after emergence or 3 weeks later). All seven trials with earlies were irrigated as required, whilst different irrigation regimes (moderate versus intensive) were compared in two trials with semi-earlies. No benefit was derived from splitting the N application. Haulm growth and N uptake increased in all cases almost linearly up to the highest N level, but tuber yield did not respond in the same way. The optimum N level was 80 kg N ha^-1 for a yield of 15 Mg ha^-1, rising to 120 kg N ha^-1 for a yield of 40 Mg ha^-1. Tuber quality was lowered by the use of excess N fertilizer, particularly in the case of earlies. The quantity of mineralised N present in the soil after harvest rose sharply with above optimum fertilizer use, and the amount of N present in crop residues also increased. The likely leaching after early potatoes was estimated to be up to 80 kg N ha^-1. The proportion of fertilizer N which was not accounted for in either tuber yield, crop residues or mineral N in soil was 26% in earlies and 38% in semi-earlies.  相似文献   

Identification of infrared absorption spectral characteristics of synovial fluid of horses with osteochondrosis of the tarsocrural joint     

Vijarnsorn M  Riley CB  Ryan DA  Rose PL  Shaw RA 《American journal of veterinary research》2007,68(5):517-523

OBJECTIVE: To determine the feasibility of the use of Fourier-transform infrared (FTIR) spectroscopy within the midinfrared range to differentiate synovial fluid samples of joints with osteochondrosis from those of control samples. ANIMALS: 33 horses with osteochondrosis of the tarsocrural joint and 31 horses free of tarsocrural joint disease. PROCEDURES: FTIR spectroscopy of synovial fluid was used. Sixty-four synovial fluid samples from the tarsocrural joint were collected. Of these, 33 samples were from horses with radiographic evidence of osteochondrosis of the tarsocrural joint and 31 from control joints. Disease-associated features within infrared spectra of synovial fluid were statistically selected for spectral classification, and the variables identified were used in a classification model. Linear discriminant analysis and leave-one-out cross-validation were used to develop a classifier to identify joints with osteochondrosis. RESULTS: 12 significant subregions were identified that met the selection criteria. The stepwise discriminant procedure resulted in the final selection of 6 optimal regions that most contributed to the discriminatory power of the classification algorithm. Infrared spectra derived from synovial fluid of joints with osteochondrosis were differentiated from the control samples with accuracy of 77% (81% specificity and 73% sensitivity). CONCLUSIONS AND CLINICAL RELEVANCE: The disease-associated characteristics of infrared spectra of synovial fluid from joints with osteochondrosis may be exploited via appropriate feature selection and classification algorithms to differentiate joints with osteochondrosis from those of control joints. Further study with larger sample size including age-, breed-, and sex-matched control horses would further validate the clinical value of infrared spectroscopy for the diagnosis of osteochondrosis in horses.  相似文献   

The source of autumn and spring downstream migrating juvenile Atlantic salmon in a small lowland river     

Anton T. Ibbotson  William D. Riley  William R.C. Beaumont  Alastair C. Cook  Mark J. Ives  Adrian C. Pinder  Luke J. Scott 《Ecology of Freshwater Fish》2013,22(1):73-81

Over 3 years, 32,444 age‐0 group Atlantic salmon (Salmo salar) parr tagged with passive integrated transponder tags throughout the River Frome catchment were assigned to one of three groups, nonmigrants, autumn migrants and spring smolts, depending on the detection and the timing of detection at downstream tag readers (situated 8.6 km above the tidal limit). We examined the effect of density at the time of tagging (n·m^?2), distance upstream from the tidal limit (km), fish length (mm), Fulton condition index, habitat type (divided into two types, main river and carrier), days after 1 September that each fish was tagged and year (replication) on the proportions of fish in each of the migration groups. Distance upstream from tidal limit was strongly negatively related to the proportion of autumn migrants and positively related to the proportion of spring smolts. Nonmigrants had a lower average body size than migrants, although there were no differences in the sizes of autumn migrants and spring smolts in September prior to migration. Fish density had no effect on migration strategy. A lower proportion of fish migrated as autumn migrants from the smaller carrier habitats than the main river channel. There is some evidence that those parr destined to become autumn migrants underwent a lower mortality rate during September before tagging than those destined to become spring migrants indicating possible physiological or behavioural differences between these two groups of fish at that time. More research into the factors responsible for initiating the autumn migration is required.  相似文献   

Early harvest and ensilage of forage sorghum infected with ergot (Claviceps africana) reduces the risk of livestock poisoning     

BJ Blaney  MJ Ryley  BD Boucher 《Australian veterinary journal》2010,88(8):311-312

Sorghum ergot produces dihydroergosine (DHES) and related alkaloids, which cause hyperthermia in cattle. Proportions of infected panicles (grain heads), leaves and stems were determined in two forage sorghum crops extensively infected 2 to 4 weeks prior to sampling and the panicles were assayed for DHES. Composite samples from each crop, plus a third grain variety crop, were coarsely chopped and half of each sealed in plastic buckets for 6 weeks to simulate ensilation. The worst-infected panicles contained up to 55 mg DHES/kg, but dilution reduced average concentrations of DHES in crops to approximately 1 mg/kg, a relatively safe level for cattle. Ensilation significantly (P = 0.043) reduced mean DHES concentrations from 0.85 to 0.46 mg/kg.  相似文献