Digestive Enzyme Activity during Larval Development of Black Snook,Centropomus nigrescens          下载免费PDF全文

Carlos Yanes‐Roca  Mayra E. Toledo‐Cuevas  Lizbeth J. Sánchez  Ana Born‐Torrijos  Nicole Rhody  Kevan L. Main 《Journal of the World Aquaculture Society》2018,49(3):612-624

Black snook, Centropomus nigrescens, have been identified as a promising candidate for aquaculture although, like many of the Centropomid species, high mortality associated with early larval stages presents a significant bottleneck to their commercialization. The digestive capacity of black snook larvae throughout the first 37 d after hatch (d.a.h.) was evaluated by quantifying digestive enzyme activities using biochemical techniques. Results showed that black snook larvae have alkaline proteases at hatching, which are known to be important during the first days of feeding for digestion. Toward the end of the study, acid proteases concentration increased (37 d.a.h.). Enzymes for lipid digestion, pancreatic lipase and bile salt‐activated lipase, were already present in the larvae before exogenous feeding commenced, and their activity increased with age and growth (length). Intracellular digestion, measured as the activity of leucine‐alanine peptidase, was high early on (5 d.a.h.) and decreased as development progressed (next 32 d). In contrast, alkaline phosphatase activity was lowest at first feeding and subsequently increased with age. Overall patterns in enzyme activity suggest the possibility of live feed weaning before 32 d.a.h. if artificial diets can be properly balanced.  相似文献   

Veterinary ethics: filling a gap in undergraduate education     

Thornton PD  Morton DB  Main DC  Kirkwood JK  Wright B 《The Veterinary record》2001,148(7):214-216

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Ethical decision-making in veterinary practice     

Mullan S  Main D 《The Veterinary record》2001,149(11):339-340

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Evaluation of peritoneal fluid pH, glucose concentration, and lactate dehydrogenase activity for detection of septic peritonitis in horses     

Van Hoogmoed L  Rodger LD  Spier SJ  Gardner IA  Yarbrough TB  Snyder JR 《Journal of the American Veterinary Medical Association》1999,214(7):1032-1036

OBJECTIVE: To determine whether peritoneal fluid pH, glucose concentration, and lactate dehydrogenase activity can be used to differentiate horses with septic peritonitis from those with nonseptic peritonitis. DESIGN: Prospective study. ANIMALS: 46 horses, including 10 healthy horses, 15 horses with septic peritonitis, and 21 horses with nonseptic peritonitis. PROCEDURE: Peritoneal fluid and blood samples were analyzed for pH, glucose concentration, and lactate dehydrogenase activity. Complete blood cell counts were performed, and peritoneal fluid samples were submitted for bacterial culture. RESULTS: Horses with septic peritonitis had significantly lower peritoneal fluid pH and glucose concentrations than horses with nonseptic peritonitis and healthy horses. Compared with other tests, serum-to-peritoneal fluid glucose concentration differences > 50 mg/dl had the highest diagnostic use for detection of septic peritonitis. Peritoneal fluid pH < 7.3, glucose concentration < 30 mg/dl, and fibrinogen concentration > 200 mg/dl were also highly indicative of septic peritonitis. CLINICAL IMPLICATIONS: Peritoneal fluid pH and glucose concentration can be used to assist in the identification of horses with septic peritonitis. These measurements can provide an early indication of sepsis, especially if cytologic evaluation of peritoneal fluid is unavailable or results are equivocal and peritoneal fluid bacterial culture results are pending.  相似文献   

Clinical evidence in the evaluation of presale radiography: are we in a desert on a horse with no name?     

Bladon BM  Main JP 《Equine veterinary journal》2003,35(4):341-342

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Does the Presence of Detached Root Border Cells of Zea mays Alter the Activity of the Pathogenic Nematode Meloidogyne incognita?     

Rodger S  Bengough AG  Griffiths BS  Stubbs V  Young IM 《Phytopathology》2003,93(9):1111-1114

ABSTRACT The root-knot nematode Meloidogyne incognita is a major pathogen of a range of important crops. Currently, control is typically achieved by the use of nematicides. However, recent work suggests that manipulating the ability of roots to slough off border cells, which then act as a decoy to the nematode, can significantly decrease damage to the roots. We investigated the attractiveness of border cells to M. incognita and the response of the nematode to border cells in close proximity. We found very limited attraction, in that nematodes did not preferentially alter direction to move toward the border cells, but a large and significant increase in nematode speed was observed once they were in the immediate vicinity of border cells. We discuss the results in the context of physical and biological mechanisms in relation to the control of pathogenic nematodes.  相似文献   

Retrospective study of the relationships between age, inflammation and the isolation of bacteria from the lower respiratory tract of thoroughbred horses     

Chapman PS  Green C  Main JP  Taylor PM  Cunningham FM  Cook AJ  Marr CM 《The Veterinary record》2000,146(4):91-95

A total of 1235 tracheal aspirates taken from 724 thoroughbreds in race training, aged from two to 10 years, were examined cytologically and bacteriologically. An inflammation scoring system on a scale of 0 to 9 was devised to allow the severity of lower airway disease to be assessed from the cytological results. The inflammation scores were closely related to the isolation of bacteria (P<0.001), and the most common bacterial isolates were Streptococcus zooepidemicus, Streptococcus pneumoniae and Pasteurella/Actinobacillus-like species. Lower airway disease was less common in older horses (P = 0.031), and the groups at highest risk were the two- and four-year-olds. Lower airway inflammation was more common in the four-year-olds at National Hunt yards than in the four-year-olds at flat racing yards (P = 0.040, odds ratio = 3.80).  相似文献   

Cryptococcosis in Gilbert's and long-nosed potoroo     

R J Vaughan  S D Vitali  P A Eden  K L Payne  K S Warren  D Forshaw  J A Friend  A M Horwitz  C Main  M B Krockenberger  R Malik 《Journal of zoo and wildlife medicine》2007,38(4):567-573

Two cases of fatal cryptococcosis are described, one of Cryptococcus neoformans infection in a Gilbert's potoroo (Potorous gilbertii) and one of Cryptococcus gattii infection in a long-nosed potoroo (Potorous tridactylus). The diagnoses were confirmed by culture and specific immunohistochemistry, respectively. The long-nosed potoroo tested positive using the latex cryptococcal antigen test (LCAT), whereas the Gilbert's potoroo had a negative LCAT result despite having advanced disease of some duration. In both cases, the clinical presentation was a progressive neurologic disease associated with a central nervous system infection. Pulmonary infection was also observed in the long-nosed potoroo. Specific treatment with antifungal agents was unsuccessful in the long-nosed potoroo.  相似文献   

Systemic iridovirus infection in freshwater angelfish, Pterophyllum scalare (Lichtenstein)   总被引：2，自引：0，他引：2  

H.D. Rodger  M. Kobs  A. Macartney  & G.N. Frerichs 《Journal of fish diseases》1997,20(1):69-72

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Resolution of a Left Ureteral Stone Using Electrohydraulic Lithotripsy in a Thoroughbred Colt   总被引：1，自引：0，他引：1  

Lynn D. Rodger  Gary P. Carlson  Michael E. Moran  Tom B. Yarbrough  John R. Pascoe  Jay A. Reynolds 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(4):280-282

A 3-year-old Thoroughbred colt was presented for evaluation of azotemia and anorexia. Physical examination revealed a ureterolith in the left ureter, approximately 10 cm from the bladder, which was thought to obstruct urine flow by approximately 90% when viewed cystoscopically. Ultrasonographic examination of both kidneys revealed indistinct corticomedullary junctions, and the right kidney was more hyperechoic. A percutaneous biopsy of the right kidney revealed chronic interstitial nephritis with marked interstitial medullary fibrosis. Medical therapy consisting of IV fluids, sodium chloride PO, and ammonium chloride PO was initiated. Ureteroscopic electrohydraulic lithotripsy via a perineal urethrostomy was used to successfully remove the stone. Klebsiella oxytoca , which responded to oral enrofloxacin therapy, was cultured from the urine after surgery. Azotemia resolved and the horse resumed training.  相似文献