排序方式： 共有18条查询结果，搜索用时 187 毫秒
1.
Pinto RD Nascimento DS Vale Ad Santos NM 《Veterinary immunology and immunopathology》2006,110(1-2):169-177
In this work, the gene and cDNA of the sea bass CD8alpha have been isolated and characterized. The coding sequence has an ORF of 666 bp. It retains the Ig motif that interacts with MHC and the two cysteines responsible for an intra-chain disulfide bridge. The hinge region contains the two essential cysteines involved in dimerization. The transmembrane region is well conserved in all analysed sequences. Similar to other teleosts, the cytoplasmic region lacks the consensus p56(lck) motif common in higher vertebrates. Analysis of the expression pattern using RT-PCR shows the highest expression in the thymus. Like in the human gene, the sea bass CD8alpha genomic structure is organized into six exons, which roughly correspond to separate functional domains of the protein. Southern blotting shows that CD8alpha exists as a single copy gene. Together, these results support the concept that the basic structure of CD8alpha has been maintained through evolution. 相似文献
2.
3.
Ruben Avendao‐Herrera Eloisa Arias‐Muoz Vernica Rojas Alicia E. Toranzo Matías Poblete‐Morales Claudio Crdova Rute Irgang 《Journal of fish diseases》2019,42(10):1447-1455
Vibrio ordalii is an extracellular, Gram‐negative bacterium that produces vibriosis in salmonids. While pathogenesis is not fully understood, this bacterium has numerous likely genes for adhesion, colonization, invasion factors and, as recently suggested, intracellular behaviour. Therefore, this study aimed to clarify possible intracellular behaviour for V. ordalii Vo‐LM‐18 and ATCC 33509T in the fish‐cell lines SHK‐1 and CHSE‐214. Confocal microscopy revealed Vo‐LM‐18 and ATCC 33509T inside cytoplasm in both fish‐cell lines at 4 hr post‐inoculation (hpi). At 8 and 16 hpi, the proportion of fish cells invaded by both strains increased. Moreover, intracellular V. ordalii were observed after 8 hpi inside mouse embryonic fibroblasts (MEF), demonstrating that entry was not due to a cellular phagocytosis process. Flow cytometry confirmed immunocytochemistry results, with both V. ordalii evidencing statistically significant differences in the number of infected cells between 8 and 16 hpi. Interestingly, V. ordalii infection did not significantly damage fish cells, as determined by LDH liberation. Viable counts at 8 hpi detected, on average for both lines, 176 ± 47 CFU/ml of culturable intracellular Vo‐LM‐18 and ATCC 33509T cells. These in vitro findings support the facultative intracellular behaviour of V. ordalii and may be of importance for understanding pathogenicity and survival in aquatic environments. 相似文献
4.
Anderson RM Latorre-Esteves M Neves AR Lavu S Medvedik O Taylor C Howitz KT Santos H Sinclair DA 《Science (New York, N.Y.)》2003,302(5653):2124-2126
Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR. 相似文献
5.
生长抑素（Somatostatin,SST）是一类神经激素多肽,对脊椎动物的生长和代谢起着关键的调控作用。在斑马鱼（Danio rerio）和其它鱼类中,生长抑素由6个基因编码（sst1-sst6）,sst1存在于所有脊椎动物中,并且在进化上最为保守。本研究利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素基因sst1突变体鱼系。通过比较6 dpf（days post fertilization,dpf）的sst1-/-突变体和野生型仔鱼转录组,发现sst1-/-突变体相关生化过程和信号通路发生了显著变化。相比野生型,sst1-/-突变体仔鱼中有354个基因显著上调,504个基因显著下调。GO（Gene ontology）富集分析表明,大部分差异基因与氨基酸和蛋白质生物合成相关。KEGG（Kyoto encyclopedia of genes and genomes）通路富集显示代谢通路、氨基酸合成、氨基酰基- tRNA合成、核糖体合成和rRNA加工相关通路表达增强。结果表明,sst1-/-突变体蛋白质生物合成和代谢过程更加活跃。RT-qPCR验证结果显示,随机选取的10个基因mRNA表达变化趋势与转录组测序结果一致,说明转录组测序结果真实可靠。6月龄突变体体重体长和野生型相比并无显著差异。本研究结果为进一步挖掘生长抑素基因家族的潜在功能奠定基础。 相似文献
6.
Marina Solé Maria D. Gómez Alfonso Martínez Galisteo Rute Santos Mercedes Valera 《Journal of Equine Veterinary Science》2013
This paper describes the handled walking and trotting kinematics (linear, temporal, and angular traits) of 35 Menorca Purebred (MEN) stallions, and the relationships among these variables is presented for the first time, along with a discussion of the influence of the hind limb pastern angle on kinematic variables at both gaits. For data collection, all animals, aged between 3 and 10 years old and belonging to 28 different studs, were recorded under the same experimental and environmental conditions, using a three-dimensional (3D) semiautomatic movement analysis system. A total of 24 kinematic variables (temporal, linear, and angular) at the walk and the trot and a morphometric variable measured at the mid stance position of walking (hind pastern angle) were included in this analysis. Angle-time diagrams of the hind pastern angle while walking and trotting normalized to stride duration were also obtained. Generally the MEN stallions' forelimb movements closely resembled the movement characteristics of other European dressage performance breeds, while the hind limb locomotion showed a greater likeness to Iberian dressage Purebreds. Despite this, their ability in collection and propulsion at the walk and the trot was relatively low. The hind limb pastern conformation was partially connected to the hind limb movements for both gaits, with an apparently negative effect of excessively upright pasterns on the amplitude at the trot, which indirectly reduced collection ability. 相似文献
7.
Cesar Ortega Leticia Caas‐Lopez Rute Irgang Raúl Fajardo Matías Poblete‐Morales Benjamin Valladares-Carranza Diana Tapia‐Cammas Ruben Avendao‐Herrera 《Journal of fish diseases》2019,42(5):667-675
Spring viraemia of carp (SVC) is an infectious disease responsible for severe economic losses for various cyprinid species, particularly common carp (Cyprinus carpio carpio). The causative agent is the SVC virus (SVCV), a member of the Sprivivirus genus, Rhabdoviridae family, and a List 1 pathogen notifiable by the World Organization for Animal Health. This study describes the diagnosis of an SVCV pathogen isolated in October 2015 from wild common carp inhabiting a natural lagoon in central Mexico. While neither an epidemic nor fish mortalities were reported, the collected killed specimens exhibited clinical signs of disease (e.g., exopthalmia, moderate abdominal distension and haemorrhaging, as well as internal haemorrhages and adhesions). Histological results of injuries were consistent with the pathology caused by SVCV. This finding was supported by the isolation of a virus in EPC and BF‐2 cells and subsequent RT‐PCR confirmation of SVCV. The phylogenetic analyses of partial SVCV glycoprotein gene sequences classified the isolates into the Ia genogroup. These findings make this the first report of SVCV detection in Mexico, extending the southern geographical range of SVCV within North America. However, since this pathogen was detected in fish inhabiting a natural body of water without tributaries or effluents, it is difficult to estimate the risk of SVCV for other wild/feral cohabitating cyprinid species in the lagoon. The status of this virus is also unknown for other bodies of water within this region. 相似文献
8.
Evaluation of a simple sedimentation method (modified McMaster) for diagnosis of bovine fascioliosis
A coprological sedimentation method is evaluated for quantification of egg shedding in bovine faeces. Through the inclusion of different number of Fasciola hepatica eggs in negative faeces, egg recovery rate and the sensitivity of the method were determined. The mean egg recovery rate of the technique was 76.72+/-15.42%. The sensitivity of the method was 33.3% whenever eggs/g of faeces (EPG) are less than 1.5 and 100% for higher values. To improve the diagnostic accuracy with this technique, it is advisable to increase the sample from 10 to 30g of faeces when manipulating low egg shedding, which allowed for a sensitivity of 83.3%. Regression equations were calculated to quantify the relationship between the number of recovered and incorporated eggs. 相似文献
9.
Aguiar Alice O. Duarte Rute A. Ladeira Ana Claudia Q. 《Water, air, and soil pollution》2013,224(9):1-8
The successive alkalinity-producing passive system (SAPPS) located in Gangneung, South Korea was designed to treat acid mine drainage. The performance of SAPPS has been monitored intensively for 3 years at the component level (influent, settling pond A, the successive alkalinity-producing system (SAPS), settling pond B, constructed wetland, and effluent). This study evaluated the ability of SAPPS to remove acidity and iron from influents at flow rates ranging from 17 to 160 m3/day. The concentration of soluble Fetotal was the highest, and the pH was the lowest at low flow rates (≤61 m3/day). When flow rates were over 80 m3/day, concentrations decreased and Fetotal was removed primarily at the SAPS stage. For flow rates of less than 61 m3/day, Fetotal was removed at the SAPS stage as well as in settling pond B and at the constructed wetland. Hydraulic retention times of 1 and 2 days were found to be appropriate and economical for use with the SAPS stage and for settling pond B and the constructed wetland, respectively The treatment of acid mine drainage by conventional SAPPSs is limited by the availability of alkaline materials. However, the new proposed system can address this weakness through the provisioning of a suitable alkalinity supply. 相似文献
10.
Rute Cesário Holger Hintelmann Nelson J. O’Driscoll Carlos E. Monteiro Miguel Caetano Marta Nogueira Ana M. Mota João Canário 《Water, air, and soil pollution》2017,228(7):257
Mercury (Hg) dynamics was evaluated in contaminated sediments and overlying waters from Tagus estuary, in two sites with different Hg anthropogenic sources: Cala Norte (CNOR) and Barreiro (BRR). Environmental factors affecting methylmercury (MMHg) production and Hg and MMHg fluxes across sediment/water interface were reported. [THg] and [MMHg] in solids (0.31–125 μg g?1 and 0.76–201 ng g?1, respectively) showed high variability with higher values in BRR. Porewater [MMHg] (0.1–63 ng L?1, 0.5–86% of THg) varied local and seasonally; higher contents were observed in the summer campaign, thus increasing sediment toxicity affecting the sediment/water Hg (and MMHg) fluxes. In CNOR and BRR sediments, Hg availability and organic carbon were the main factors controlling MMHg production. Noteworthy, an upward MMHg diffusive flux was observed in winter that was inverted in summer. Although MMHg production increases in warmer month, the MMHg concentrations in overlying water increase in a higher proportion compared to the levels in porewaters. This opposite trend could be explained by different extension of MMHg demethylation in the water column. The high concentrations of Hg and MMHg and their dynamics in sediments are of major concern since they can cause an exportation of Hg from the contaminated areas up to ca. 14,600 mg year?1 and an MMHg deposition of up to ca. 6000 mg year?1. The results suggest that sediments from contaminated areas of Tagus estuary should be considered as a primary source of Hg for the water column and a sink of MMHg to the sedimentary column. 相似文献