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Vine-1 retrotransposon-based sequence-specific amplified polymorphism for Vitis vinifera L. genotyping 总被引：1，自引：0，他引：1
The sequence‐specific amplification polymorphism (S‐SAP) method, derived from the amplified fragment length polymorphism (AFLP) technique, produces amplified fragments containing retrotransposon long terminal repeat ( LTR ) sequence at one end and a host restriction site at the other. The development and application of this procedure to the LTR of the Vine‐1 element from grapevine is reported. Two primers derived from one of the LTR sequences flanking the retrotransposon were used in combination with MseI degenerated primers on 15 grapevine accessions. S‐SAP results were compared with AFLP data. The heterozygosity and gene diversity values were higher for S‐SAP than for the AFLP procedure. Results show that S‐SAP amplification is effective in identifying polymorphisms and defining genetic distances among cultivars, and could be used for fingerprinting and for ‘Traminer’ clone identification. To the contrary Vine‐1 retrotransposon‐based S‐SAP was not able to distinguish ‘Pinot’ clones. 相似文献
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Luca MALFASSI Francesca FIDANZIO Massimo SALA Silvia MARCARINI Giovanni MAZZA Nancy CARRARA Simone PAVESI Giacomo GNUDI Gaetano URSO Mario DOLERA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):695
The aims of this pilot study were to evaluate the feasibility and efficacy of high-dose hypofractionated volumetric modulated arc radiotherapy (VMAT) applied to whole pelvic region radiotherapy (WPRT) with multilevel simultaneous integrated boost (MLSIB) combined with piroxicam and chemotherapy for the treatment of canine transitional cell carcinoma (TCC) of the lower urinary tract with muscle invasion TCC. Twelve dogs were enrolled, according to stage, in two groups: group 1, TCC confined to the urinary tract; group 2, TCC with metastasis. The planning target volume dose was tailored from 36 to 42 Gy in 6 fractions. All dogs were prescribed piroxicam and radiosensitizing carboplatin, and six received chemotherapy after radiotherapy. Serial follow-ups with computed tomography and magnetic resonance imaging were performed. Disease control and toxicity effects were evaluated according to the Response Evaluation Criteria in Solid Tumors and Veterinary Radiation Therapy Oncology Group criteria. The treatment was well tolerated, and no high-grade side effects were reported. The median overall survival times for groups 1 and 2 were 1,230 and 150 days, respectively. A considerable percentage of patients in group1 (50%) were still alive at the time of writing this paper, and a longer follow-up could enable a more accurate survival analysis. This preliminary analysis shows that VMAT applied to the WPRT with MLSIB is an effective and safe option for dogs with lower urinary TCC, although the presence of metastases worsens the prognosis. 相似文献
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Measurements of exchangeable K, percentage of exchangeable K in the total exchange capacity, K extracted in a CaCl2 solution, and the change in free energy of exchange of K for Mg and Ca (ΔF) were compared as methods for the evaluation of available K in soils. The evaluation was based on the K uptake of plants in a pot exhaustion experiment. The ΔF value was best correlated with the K uptake of the plants. A good correlation was found between the logarithms of the exchangeable K expressed as a percentage of the cation exchange capacity and ΔF values. 相似文献
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FRANCESC HERNANDEZ‐SANCHO VICENT SOLER‐MARCO RAMÓN SALA‐GARRIDO VICENT ALMENAR‐LLONGO 《Growth and change》2012,43(2):179-197
A series of works have analysed differential behaviour in terms of productive efficiency between companies inside a hypothetical industrial district and those outside the district. This objective has been addressed using measures of technical efficiency. The results obtained provide valuable information for quantifying the district effect at a given moment in time. However, constant changes in the market and business behaviour mean that it is worthwhile studying the business district effect from a dynamic point of view. In this study, we provide this new vision through the use of Malmquist productivity indices. This methodology enables us to analyse possible differential evolutions by comparing the productive efficiency of companies inside and outside a district over a period of time. An empirical application has been made on a set of small and medium‐sized Spanish ceramic producers during the period of economic expansion between 1996 and 2007. 相似文献
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