全文获取类型
收费全文 | 176篇 |
免费 | 13篇 |
专业分类
林业 | 23篇 |
农学 | 12篇 |
基础科学 | 2篇 |
41篇 | |
综合类 | 8篇 |
农作物 | 11篇 |
水产渔业 | 10篇 |
畜牧兽医 | 69篇 |
园艺 | 7篇 |
植物保护 | 6篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 11篇 |
2017年 | 10篇 |
2016年 | 8篇 |
2015年 | 8篇 |
2014年 | 4篇 |
2013年 | 14篇 |
2012年 | 12篇 |
2011年 | 17篇 |
2010年 | 10篇 |
2009年 | 12篇 |
2008年 | 12篇 |
2007年 | 13篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1991年 | 1篇 |
排序方式: 共有189条查询结果,搜索用时 203 毫秒
1.
The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E(1)S) and progesterone (P(4)) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E(1)S and P(4) by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E(1)S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E(1)S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E(1)S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (P<0.05) P(4) concentrations than the average. These results raise the possibility that the E(1)S and P(4) profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation. 相似文献
2.
3.
Kumar Chavlesh Singh Sanjay Kumar Verma Mahendra Kumar Pramanick Kallol Kumar Srivastav Manish Kaur Sukhdeep Kumar Rajeev Ranjan 《Genetic Resources and Crop Evolution》2022,69(2):645-659
Genetic Resources and Crop Evolution - The wild Malus germplasm is considered as a gene reservoir for various biotic and abiotic stresses tolerance/resistance genes, including important novel... 相似文献
4.
Kapil Malik Deepshikha Birla Honey Yadav Manish Sainger Darshna Chaudhary Pawan K. Jaiwal 《Journal of Crop Science and Biotechnology》2017,20(3):185-192
Various factors affecting in vitro regeneration like different carbon sources, different gelling agents, and growth additives were assessed comprehensively for callus induction and plant regeneration for five Indian wheat cultivars using mature embryos as the explants for the first time. The tissue culture responses of cultivars WH-1105, HD-2967, and PBW-343 have not been reported earlier. Besides, the effect of different concentrations of the cytokinin, zeatin has also been optimized. Using the optimized factors, the efficiency of five different varieties, i.e., HD 2967, C 306, RAJ 3765, WH 1105, and PBW 343 was evaluated for regeneration. Modified MS basal medium containing dicamba reduced precocious germination of the embryo and induced embryogenic callus more efficiently. Removal of embryogenic calli from non-regenerable structures during early callus phase improved plant regeneration. These calli on zeatin (1.0 mgl-1) and dicamba (0.1 mgl-1) containing medium showed the highest regeneration frequency (98%) with a maximum of 8-9 shoots per calli. Maltose had the maximum callusing and regeneration percentage than other carbon sources. Various gelling agents did not have any significant difference on the regeneration. Of all the varieties, C-306 and HD-2967 were found to be more regenerative and can be used in transformation experiments. 相似文献
5.
Genetic analysis and pyramiding of two gall midge resistance genes (Gm-2 and Gm-6t) in rice ( Oryza sativa L.) 总被引:4,自引:0,他引:4
Sanjay Katiyar Satish Verulkar Girish Chandel Yang Zhang Bingchao Huang John Bennett 《Euphytica》2001,122(2):327-334
Asian rice gall midge (Orseolia oryzae) is a major pest across much of south and southeast Asia. This pest is genetically diverse and many gall midge biotypes are
known to exist in each country. During the last three decades, host plant resistance has proved to be the most effective mechanism
of controlling the Asian rice gall midge. Seven genes conditioning resistance to gall midge larvae have been identified in
rice (Oryza sativa) and are being used in cultivar improvement programs. However, some of these genes are rendered ineffective by new gall midge
biotypes. Increased understanding of genetics, inheritance, allelic relationships and linkage is necessary to maximise the
durability of major gene resistance by the pyramiding of these genes. The two genes, Gm-2 and Gm-6(t), are known to confer resistance against a number of biotypes in India and China, respectively. An F3 population derived from a cross between Duokang #1 (donor of Gm-6(t)) and Phalguna (donor of Gm-2) was screened against Chinese gall midge biotype 4 at Guangdong, China, and Indian gall midge biotype 1 at Raipur, India.
At each location, separately,a single gene governed resistance. The parallel segregation of 417 F3progenies for both biotypes at two locations revealed that recombination had occurred between the two genes, establishing
that the two genes are not allelic. However, the two genes Gm-2 and Gm-6(t), were found to be linked with a distance of ∼16.3 cM. A number of lines homozygous at one locus and segregating for the other
locus were identified and selected. These lines were selfed to obtain lines homozygous for the favourable alleles at both
loci (two locus pyramids). This is the first report on use of conventional host-pest interaction method for pyramiding two
closely located Gm-resistance loci of dissimilar effects. The implications of deployment of these pyramids within and across
country borders, with reference to the prevailing gall midge populations are discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
6.
Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers 下载免费PDF全文
Kapil K. Tiwari Anshuman Singh Sasmita Pattnaik Maninder Sandhu Sukhdeep Kaur Sourabh Jain Sushma Tiwari Shweta Mehrotra Mahender Anumalla Rashmita Samal Jyoti Bhardwaj Neha Dubey Vikrant Sahu Gayle A. Kharshing Patu K. Zeliang Kadiri Sreenivasan Pankaj Kumar Swarup K. Parida Sevanthi V. A. Mithra Vandana Rai Wricha Tyagi Pawan K. Agrawal Atmakuri R. Rao Arunava Pattanayak Girish Chandel Ashok K. Singh Ishwari S. Bisht Kangila V. Bhat Gundimeda J. N. Rao Jitendra P. Khurana Nagendra K. Singh Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171
Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining. 相似文献
7.
Kim JY Yokoyama N Kumar S Inoue N Yamaguchi T Sentoku S Fujisaki K Sugimoto C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):251-256
Benign Theileria species of cattle are found in most parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a maker for epidemiological and phylogenetical studies of benign Theileria species. Parasites with Ikeda- or Chitose-type MPSP genes are dominant in Japan, but we report here mixed infection cases of Theileria parasites with an additional MPSP type parasite infecting cattle in Abashiri District, Hokkaido. The MPSP gene sequence found in the additional type was closely related to MPSP genes of Theileria parasites found in Southeast Asian countries, including Thailand (Narathiwat) and Indonesia (Java). Theileria parasites from the blood sample were also distinguishable from the Ikeda or Chitose type parasites by the small subunit (SSU) rRNA gene sequence analysis, and they are grouped into the SSU rRNA types C/D found in Korea, North America, and Spain. The present finding of mixed infections of cattle with three different types of Theileria makes epidemiological feature of bovine theileriosis in Japan more complex. We have designed a set of primers specific to this MPSP type in order to conduct further epidemiological study. 相似文献
8.
9.
Kalia K Sharma K Singh HP Singh B 《Journal of agricultural and food chemistry》2008,56(21):10129-10134
The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential (FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to 41.43) mg of Trolox/g] with ABTS (*+), DPPH (*) and FRAP assays, respectively. In general, TEAC of Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly (1.2 times) higher than microwave. A positive correlation (r(2) = 0.931 to 0.982) was observed between total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity. In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also quantified in different extracts by RP-HPLC. 相似文献
10.
Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found. 相似文献