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Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the...  相似文献   
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Seven species of Solanum were screened for their molluscicidal properties against Biomphalaria glabrata, the intermediated host of Schistosoma mansoni, Solanum agrarium, S. jabrense, S. melissarum, S. megalonyx, S. paludosum, S. paraibanum and S. stipulaceum. Four extracts showed molluscicidal activity with LC(50) from 22 to 56 microg/ml.  相似文献   
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The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.  相似文献   
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Genotoxic and antigenotoxic effects of acerola fruit at two stages of ripeness were investigated using mice blood cells. The results show that no ripeness stage of acerola extracts presented any genotoxic potential to damage DNA (Comet assay) or cytotoxicity (MTT assay). When antigenotoxic activity was analyzed, unripe fruit presented higher DNA protection than ripe fruit (red color) extract. The antioxidant capacity of substances also showed that unripe samples inhibit the free radical DPPH more significantly than the ripe ones. The results about determination of compounds made using HPLC showed that unripe acerola presents higher levels of vitamin C as compared to ripe acerola. Thus, vitamin C and the complex mixture of nutrients of Malpighia glabra L., and especially its ripeness stages, influenced the interaction of the fruit extract with the DNA. Acerola is usually consumed when ripe (red fruit), although it is the green fruit (unripe) that has higher potential as beneficial to DNA, protecting it against oxidative stress.  相似文献   
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This study evaluated the starch digestibility, physicochemical properties and cooking characteristics of polished rice (varieties IRGA417 and IAC202) subjected to the doses of 0 (as the control), 1, 2 and 5 kGy of gamma radiation. The highest dose decreased the apparent amylose content, peak viscosity, water absorption and volume expansion. Irradiation increased the solid loss by 119% and 187% for IAC202 and IRGA417, respectively, when comparing the higher dose with the control. For IAC202, irradiation decreased the rapidly digestible starch and increased the slowly digestible starch (SDS) and resistant starch (RS). IRGA417 showed an elevation of SDS and a reduction in RS. And 1 kGy dose of gamma radiation generated the highest level of RS for both the two varieties and presented the smallest changes in other physicochemical and cooking properties.  相似文献   
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Thiamethoxam (TMX) is one of the most widely used neonicotinoid insecticides for pest control in agricultural crops. However, information on its acute and chronic toxicity for freshwater non-target insects is still limited. We aimed to evaluate the lethal and sub-lethal effects of TMX-based insecticide on Chironomus xanthus using laboratory ecotoxicological assays. Besides survival, 28-day partial life-cycle tests assessed the effects of chronic exposures on larval growth and emergence of midges. The estimated 48-h LC50 was 32 μg a. i./L of TMX. Chronic tests revealed that chironomids exposed to sub-lethal concentrations of TMX showed reduced larval growth (LOEC?=?0.4 and 1.6 μg a.i/L of TMX for head capsule width and growth rate, respectively) and emergence rate (LOEC of 1.6 μg a.i/L of TMX). This study provides important ecotoxicological data concerning effects of TMX-based insecticides on tropical ecosystems and reveals that environmentally relevant concentrations of TMX can adversely affect chironomids populations. Our study also validates C. xanthus as a sensitive species to monitor the ecological integrity of tropical aquatic systems near intensive agricultural areas and to help understand the effects caused by TMX on freshwater insects.  相似文献   
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This study investigated the effect of gamma radiation on the digestibility and functional properties of rice starch. Rice cultivars IRGA417 and IAC202 were used for isolation of starch by the alkaline method. Starch samples were irradiated with 1, 2 and 5 kGy doses of 60Co at a rate of 0.4 kGy/h. A control sample, which was not irradiated, was used for comparison. Irradiated and control starches were characterized by in vitro starch digestibility, total dietary fiber, color, water absorption index, water solubility index, syneresis, swelling factor, amylose leaching, pasting properties and gel firmness. Irradiations changed starch digestibility differently in either cultivar. Increasing radiation doses promoted increase in the color parameter b* (yellow), elevation in the capacity to absorb water, and solubility in water as well as the amylose leached from granules for both cultivars. Pasting properties showed a decrease that was proportional to the dose applied, caused by the depolymerization of starch molecules. Gel firmness of the starch from IAC202 was inversely proportional to the radiation dose applied, whereas for IRGA417, there was a reduction at 5 kGy dose. Rice starches can be modified by irradiation to exhibit different functional characteristics and they can be used by the food industries in products such as soups, desserts, flans, puddings and others.  相似文献   
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