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The use of spleen antigen in the tube agglutination test for diagnosis of anaplasmosis in cattle 总被引:1,自引:0,他引:1
An antigen was prepared from the spleen of an infected bovine calf and was used in the tube agglutination test. Cases having rare and very low parasitaemia as well as the carrier cases were detected by this procedure and no non-specific reaction was observed with other haemoprotozoan infections. The maximum titre obtained with the Anplasma positive sera was 1:20, about 60 per cent showed a titre of 1:10 and 90 per cent showed a titre of 1:5 or more. The test provided satisfactory results in detecting the cases even at the early phase of the infection. The test also proved useful in judging the efficacy of a drug employed to destroy the carrier status. 相似文献
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Beniwal R. P. Nichani A. K. Rakha N. K. Sharma R. D. Sarup S. 《Tropical animal health and production》1997,29(4):124S-126S
Tropical Animal Health and Production - Bovine babesiosis, caused byBabesia bigemina, is an important tick-transmitted haemoprotozoan disease in the tropics. This study evaluated the... 相似文献
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Direct interaction of a ligand for the erbB2 oncogene product with the EGF receptor and p185erbB2 总被引:23,自引:0,他引:23
R Lupu R Colomer G Zugmaier J Sarup M Shepard D Slamon M E Lippman 《Science (New York, N.Y.)》1990,249(4976):1552-1555
The erbB2 oncogene encodes a 185-kilodalton transmembrane protein whose sequence is similar to the epidermal growth factor receptor (EGFR). A 30-kilodalton factor (gp30) secreted from MDA-MB-231 human breast cancer cells was shown to be a ligand for p185erbB2. An antibody to EGFR abolished the tyrosine phosphorylation induced by EGF and transforming growth factor-alpha (TGF-alpha) but only partially blocked that produced by gp30 in SK-BR-3 breast cancer cells. In two cell lines that overexpress erbB2 but do not expresss EGFR (MDA-MB-453 breast cancer cells and a Chinese hamster ovary cell line that had been transfected with erbB2), phosphorylation of p185erbB2 was induced only by gp30. The gp30 specifically inhibited the growth of cells that overexpressed p185erbB2. An antibody to EGFR had no effect on the inhibition of SK-BR-3 cell colony formation obtained with gp30. Thus, it appeared that gp30 interacted directly with the EGFR and erbB2. Direct binding of gp30 to p185erbB2 was confirmed by binding competition experiments, where gp30 was found to displace the p185erbB2 binding of a specific antibody to p185erbB2. The evidence described here suggests that gp30 is a ligand for p185erbB2. 相似文献
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Beniwal R. K. Nichani A. K. Sharma R. D. Rakha N. K. Suri D. Sarup S. 《Tropical animal health and production》1997,29(4):109S-113S
Tropical Animal Health and Production - Bovine tropical theileriosis caused byTheileria annulata is an economically important disease of cattle in India. The disease has assumed paramount... 相似文献
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