Performance of broiler chicks fed on alkali‐treated neem (Azadirachta indica) kernel cake as a protein supplement     

D. Nagalakshmi  V. R. B. Sastry  D. K. Agrawal  R. C. Katiyar  S. V. S. Verma 《British poultry science》1996,37(4):809-818

1. An experiment was conducted to evaluate growth and nutrient efficiency of broiler chicks from 3 to 42 d fed on diets containing alkali‐treated neem (Azadirachta indica A. juss) kernel cake (NKC) as a protein supplement in place of peanut meal (PNM).

2. NKC was treated with sodium hydroxide at 10 (ANKC 1) or 20 g (ANKC 2)/kg and incorporated into the test diets at 135 or 300 g/kg to replace 50 (low—L) or 100 (High—H) % of the PNM protein of the reference diet.

3. Despite comparable retentions of dry matter and total carbohydrate on L‐ANKC 1 and 2, fibre on L‐and H‐ANKC 2 and nitrogen, calcium and acid detergent fibre on all experimental diets compared to the retentions of chicks on the reference diet, only the chicks fed L‐ANKG 2 were found to grow and utilise food as well as those on the reference diet.

4. The activities of serum alkaline phosphatase on H‐ANKC 1 and alanine amino transferase on all test diets were depressed (P < 0.05), but the activity of serum aspartate amino transferase, total erythrocyte count and concentration of blood haemoglobin and urea were similar in all chicks.

5. No significant differences were noticed in the qualitative and quantitative characteristics of the meat of chicks fed on the reference diet and on diets incorporating ANKC at the lower concentrations. Feeding ANKC protein did not impart any untoward taste as evaluated in pressure cooked meat by a semitrained panel on a 7 point Hedonic scale.

6. Except for duodenal and jejunal inflammation in chicks on both reference and test diets, all the vital organs were normal, ruling out any adverse affects caused by residual neem bitters.

7. Comparable performance and cost of chicks fed on the reference and L‐ANKC 2 diets, warrants the utilisation of hitherto wasted protein‐rich NKC after alkali treatment in broiler chick diets to spare peanut meal for human consumption in developing countries.  相似文献   

Multivariate analysis and genetic diversity for morphometric and root textural quality traits in ashwagandha (Withania somnifera Dunal)     

R. Ramesh Kumar  L. Prasanna Anjaneya ReddyJ. Vinay Kumar  K. KomaraiahS. Purnanand  K.P. Sastry 《Industrial Crops and Products》2012,35(1):199-202

Ashwagandha (Withania somnifera) is an important medicinal plant and its dried roots are used in traditional systems of medicine. The market price of roots is determined by physical (textural) quality. Brittle roots with high starch and low fiber are considered to be superior because of ease in grinding. Genetic diversity studies based on root textural parameters have not been done so far. So the present study was designed to assess genetic diversity for morphometric traits and root textural quality parameters among two morphologically distinct groups: Poshita and Nagore. The PCA separated the morphometric and root texture variables distinctly into two different principal components: PC-1 and PC-2 respectively, indicating that both are negatively associated. All the morphotypes in Poshita group showed high positive loadings in PC-1 indicating that component genotypes are high root yielding. Nagore morphotypes were low yielding but the root texture was good. Clustering of morphotypes grouped Poshita and Nagore separately with high inter-cluster distances indicating that both groups are highly divergent from each other, suggesting that there is sufficient scope for varietal improvement through hybridization.  相似文献   

Haematological and cerebrospinal fluid findings in canine neural angiostrongylosis   总被引：1，自引：0，他引：1  

KV MASON 《Australian veterinary journal》1989,66(5):152-154

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A new method of evaluation of the potency of anti rinderpest serum     

G A Sastry 《The Indian veterinary journal》1970,47(1):7-14

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Identification and validation of cold responsive microRNAs of <Emphasis Type="Italic">Hevea brasiliensis</Emphasis> using high throughput sequencing     

Linu Kuruvilla  MB Mohamed Sathik  Molly Thomas  Lisha.P Luke  Sumesh KV 《Journal of Crop Science and Biotechnology》2017,20(5):369-377

Cold stress is one of the major abiotic factors that influence the productivity and geographical distribution of many agriculturally important crops like Hevea brasiliensis. Cultivation of H. brasiliensis in India is being extended to northeastern regions, where low temperature during winter adversely affects its survival, growth, and productivity. Developing cold-tolerant genotypes is a primary requisite to maximize the productivity under such challenging environmental conditions. However, lack of methods for early evaluation of cold tolerance in the newly developed clones and the extensive time required for assessing their tolerance in the field are major constraints for clonal selection. The present study was initiated with an objective to identify and characterize cold stress responsive miRNAs from H. brasiliensis that show stronger association with cold tolerance. Next generation sequencing using Illumina HiSeq method revealed the expression of 21 and 29 conserved miRNA (from clone RRIM 600) families in cold-stressed and control samples, respectively. Forty-two novel miRNAs were identified from this study. Upon differential expression analysis, eight conserved miRNAs were found commonly expressed in both the samples. When expression analyses were performed subsequently with six selected miRNAs in two Hevea clones (viz. RRII 105 and RRIM 600), miR169 showed a strong association with cold tolerance. miRNAs such as miR482 and miR159 also exhibited association with cold tolerance. This study suggests the possibility of employing these miRNAs as markers for cold tolerance after validation in more number of genotypes with varying levels of cold tolerance.  相似文献   

Mercuric chloride induced alterations in the intestinal transport of glucose and fructose in the fresh water murrel Channa punctatus     

K. V. Sastry  D. R. Rao 《Water, air, and soil pollution》1983,19(2):143-147

The effect of five concentrations of mercuric chloride (10 mM, 5 mM, 1 mM, 0.5 mM, and 0.l mM) on the uptake of glucose and fructose from the intestine of the fresh water murrel Channa punctatus after 15, 30 and 60 min was studied. Intestinal glucose and fructose transport was also assessed in fish exposed to a sublethal concentration (3 μg l^?1) of mercuric chloride for 10 and 30 days. The results show that the uptake of glucose and fructose from the intestine was reduced by all five concentrations of mercuric chloride. Maximum reduction was observed after 60 min of exposure to 10 mM mercuric chloride. The rate of absorption of glucose and fructose increased with time in controls and in all concentrations of mercury except the highest concentration (10 mM) where the rate of absorption decreased gradually. The reduction in the rate of absorption of the two sugars was not dependent on the concentration of mercury in the instilled medium. In fish exposed to mercuric chloride in laboratory media, the reduction of glucose and fructose absorption was greater after 30 days of exposure than after 10 days.  相似文献   

Genetic resource enhancement by isolation of diversegenotypes from seed progeny in predominantly sterile rose scentedgeranium Pelargonium graveolens     

Ritika Gupta  K.P. Sastry  S. Banerjee  G.R. Mallavarapu  Sushil Kumar 《Genetic Resources and Crop Evolution》2001,48(6):629-636

Four new accessions (BSP1-4) of rose scentedgeranium Pelargonium graveolens were derivedfrom the spontaneous half-sib seed progeny of mostly sterilepopulations of the cultivar accession Bipuli. The seed producingplants of Bipuli had been growing among the populations of cultivaraccessions Hemanti and Kunti in the temperate agroclimate ofKodaikanal, Tamilnadu in India. The four new accessions were comparedwith the cultivar accessions Bipuli, Hemanti and Kunti in fieldexperiments under subtropical agroclimate of Lucknow, Uttar Pradeshfor essential oil yield and quality related traits and in terms oftheir RAPD profiles. The crop yield and essential oil parameters andDNA profiles of the four new accessions and three cultivar accessionsallowed the conclusion that the accessions BSP-1, BSP-2 andBSP-3 were Bipuli × Kunti hybrids and BSP-4was a Bipuli × Hemanti hybrid. The accession BSP-4demonstrated hybrid vigour in several of the essential oil yieldrelated traits; on average basis it out yielded other accessions by afactor of about 2.4. The essential oil of BSP-4 had 89%rhodinol content in which citronellol to geraniol ratio was 1:1 andthe contents of isomenthone, menthone, 10-epi--eudesmol, 6,9-guaiadiene, decanoic acid andisodecanoic acid were relatively lower than in the oils of accessionsBipuli and/or Hemanti. The present study has shown a way for thegeneration of new genotypes in rose scented geranium in which thecultivars have been vegetatively maintained for decades and thegenetic resources are scanty.  相似文献   

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda   总被引：77，自引：0，他引：77  

W D Huse  L Sastry  S A Iverson  A S Kang  M Alting-Mees  D R Burton  S J Benkovic  R A Lerner 《Science (New York, N.Y.)》1989,246(4935):1275-1281

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.  相似文献   

Experimental lantana poisoning and its treatment in livestock     

M S Sastry  Y P Singh 《The Indian veterinary journal》1979,56(12):1007-1012

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Neoplasms of ducks in Andhra Pradesh. (I). A report of two cases of extra hepatic tumours     

P R Rao  K J Christopher  J Venkatanarayana  G A Sastry 《The Indian veterinary journal》1967,44(9):745-747

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