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Total haemoglobin and methaemoglobin concentration in blood was analyzed in sea bass (Dicentrarchus labrax) exposed for 18 h to 150 mg/l NO2-N in sea water. Observations of recovery mechanisms carried out for 24 days following the nitrite exposure showed that, after a rapid disappearance of methaemoglobinaemia, a strong anaemia occurred, due to a drop in the total haemoglobin concentration. Complete recovery from this anaemia occurred only after 24 days. The mechanism of recovery from nitrite intoxication is discussed with a view to devising treatment that might help to minimize losses at fish-farming facilities. 相似文献
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Static acute toxicity tests of 4 and 7 days duration were performed on sea bass (Dicentrarchus labrax) and European eel (Anguilla anguilla). The 96-hour LC50 for sea bass (x?; = 5 cm) was 274, 220, and 154 mg/liter N-NO2 at water temperatures of 17, 23 and 27°C, respectively, in sea water. The 96-hour LC50 for eel elvers was 84, 213, 264, 503, 812 and 974 at salinities of 0, 6, 12, 24, 30 and 36 g/liter. 相似文献
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Spanu V. Virdis S. Scarano C. Cossu F. De Santis E. P. L. Cosseddu A. M. 《Veterinary research communications》2010,34(1):87-90
In vitro activities of 16 antibiotics were tested against 36 Staphylococcus aureus (SA) strains isolated from raw sheep’s milk cheese from six dairies. The minimum inhibitory concentration (MIC) was determined using a broth microdilution method (CLSI). All 36 isolates were analyzed for the presence of the accessory gene regulator gene, agr (I–IV), and genes encoding resistance to methicillin (mecA), erythromycin (ermA), penicillin (blaZ), and vancomycin (vanA-B). The isolates were also analyzed for similarities in pulsed-field gel electrophoresis (PFGE) patterns. SA strains showed resistance to ampicillin (36.1%), penicillin (33.3%), tetracycline (11.1%), and cloxacillin (2.8%) but were susceptible (≥94.4%) to 12 out of 16 tested antimicrobials. The overall susceptibility of the strains to oxacillin, vancomycin, and erythromycin was confirmed by the absence of the mecA, vanA-B, and ermA genes. The PFGE results showed that 32 strains belonged to 10 different clusters (P1–P10) while four strains were untypeable. 相似文献
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Patel KD Scarano FJ Kondo M Hurta RA Neto CC 《Journal of agricultural and food chemistry》2011,59(24):12864-12873
Cranberry ( Vaccinium macrocarpon ) has been shown in clinical studies to reduce infections caused by Escherichia coli and other bacteria, and proanthocyanidins are believed to play a role. The ability of cranberry to inhibit the growth of opportunistic human fungal pathogens that cause oral, skin, respiratory, and systemic infections has not been well-studied. Fractions from whole cranberry fruit were screened for inhibition of five Candida species and Cryptococcus neoformans , a causative agent of fungal meningitis. Candida glabrata , Candida lusitaniae , Candida krusei , and Cryptococcus neoformans showed significant susceptibility to treatment with cranberry proanthocyanidin fractions in a broth microdilution assay, with minimum inhibitory concentrations as low as 1 μg/mL. MALDI-TOF MS analysis of subfractions detected epicatechin oligomers of up to 12 degrees of polymerization. Those containing larger oligomers caused the strongest inhibition. This study suggests that cranberry has potential as an antifungal agent. 相似文献
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Sergio Pietro Ferrante Sergio Lucretti Silvia Reale Alessandro De Patrizio Loredana Abbate Nicasio Tusa Maria-Teresa Scarano 《Euphytica》2010,173(2):223-233
We report the accurate determination of the allelic configurations of a total of eight new citrus tetraploid hybrids by means
of SSR analysis, coupled with capillary electrophoresis, and PCR based dosage effects. Tetraploid hybrids were spontaneously
obtained from different interploid crosses (2x × 4x) between diploid ‘Femminello’ lemon and the allotetraploid somatic hybrid
(2n = 4x = 36) ‘Key’ lime + ‘Valencia’ orange, and between diploid ‘Wilking’ and ‘Fortune’ mandarins and an autotetraploid ‘Dancy’
mandarin (2n = 4x = 36). To understand the opportunity to employ them in further backcross programs, the cytological mechanisms underlying
their ploidy level were unambiguously determined using six SSR primers. PCR conditions were optimized and skewness in template/product
ratios were verified. Tetraploid allelic configurations were determined from PCR based dosage effects using electropherogram
peak heights to estimate the copy number per allele. In all the tetraploid hybrids we found out that diploginy (2n eggs) has occurred, contributing the extra haploid genome in the tetraploids. According to the marker genotypes, it was further
inferred that the 2n eggs in ‘Femminello’ lemon resulted from first division restitution (FDR), while in ‘Wilking’ and ‘Fortune’ mandarins 2n eggs occurred in second division restitution (SDR). These new genotypes, with their improved genetic female background, can
be therefore considered very valuable in our citrus genetic improvement program as pollen donors in backcrosses suitable to
eliminate negative traits. 相似文献
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Marco D’Imperio Vincenzo Viscosi Maria-Teresa Scarano Mariasilvia D’Andrea Biagi Angelo Zullo Fabio Pilla 《Scientia Horticulturae》2011
Three olive cultivars (Oliva Nera di Colletorto, Noccioluta, and a probably a new local genotype) from two strictly related areas of Molise region (south-centre of Italy) were characterized by combining molecular data (eight SSRs analyzed on leaves) and morphological features (thirty-one parameters from leaves, drupes and pits). Both molecular and morphological analyses have shown a very good separation of the three endemic cultivars. A high correlation between morphological and molecular data was found using Mantel's test. The morphological traits of pits were less influenced by environmental pressure than the leaves and drupes; therefore, the pits are more affected by genetic control and might be considered a helpful tool for cultivar characterization and identification. Potential and limitations of three statistical models computed to perform cultivar identification by morphological measures is also discussed. We demonstrated that molecular and morphological analyses are useful for distinguishing new accessions and studying local varieties to preserve genetic diversity, even at small geographical scale in such an unequivocal way; hence the methodology could be proposed as a tool to discriminate widespread cultivars, with long genetic distances. 相似文献
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