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1.
Randomly Amplified Polymorphic DNA markers were used to determine the genetic relationships among Turkish lentil cultivars and breeding lines. Fourteen cultivars and thirteen breeding lines were evaluated to determine genetic variability using nine random 10-mer primers (among 45 primers). Forty-one reproducible bands were obtained, 54% of which were polymorphic. Genetic distances among cultivars and breeding lines were obtained from the simple matching coefficients (SM). The lowest genetic distance was observed between the cultivars of Ali Dayi and Kafkas with 5.0%, while ‘Seyran-96’ and ‘Ozbek’ had the highest genetic distance with 58.3%. An unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis was performed on the distance matrix using Phylip software. The dendrogram clearly showed two distinct groups. The first group is composed of Akm 565 and Akm 563. The second group contains all the cultivars and remaining eleven breeding lines.  相似文献   
2.
Abstract

This study was conducted to investigate the effects of garlic and ginger oils on hematological and biochemical health characteristics of sea bass Dicentrarchus labrax. Fish were exposed to garlic oil (0.01 or 0.02 mL/L), ginger oil (0.01 or 0.02 mL/L), or a combination of the two oils (each oil at a concentration of 0.005 or 0.01 mL/L) for 96 h via bath immersion. Results showed that the red blood cell count, hematocrit (%), hemoglobin (Hb) concentration (g/dL), mean corpuscular volume (μm3), mean corpuscular Hb (pg), and mean corpuscular Hb concentration (%) were not significantly affected by herb oil exposure. However, some changes in biochemical variables were observed. Sea bass exposed to the 0.005-mL/L garlic oil–ginger oil mixture exhibited a significant increase in serum glucose. Serum total protein and albumin levels decreased in sea bass that were exposed to a garlic oil–ginger oil mixture (0.005 or 0.01 mL/L) or to garlic oil at 0.02 mL/L. Serum globulin levels decreased and triglyceride levels increased in sea bass exposed to 0.02-mL/L garlic oil or to the 0.01-mL/L mixture. The serum lipase level decreased and the cholesterol level increased in fish that were exposed to 0.02-mL/L garlic oil. In summary, ginger oil at 0.01–0.02 mL/L can be used without negative effects, while the garlic oil or garlic oil–ginger oil mixture should be applied at a concentration below 0.005 mL/L for bath immersion of sea bass. This is the first study to examine how garlic oil and ginger oil exposure via bath immersion affects the hematological and biochemical status of sea bass.

Received March 19, 2012; accepted July 2, 2012  相似文献   
3.
Abstract

This three-year study (2003–2005) aimed to improve the yield and quality of pastures growing naturally that are colonized by naturally occurring vegetation without agricultural input under hazelnut (Corylus sp.) orchards in the middle and eastern Black Sea regions of Turkey. There were eight treatments: 1) control; 2) fertilizer only (triple superphosphate 44% and calcium ammonium nitrate 26%) (80kg ha?1 P and 60 kg ha?1 N in Samsun; 100 kg ha?1 P and 80 kg ha?1 N in Ordu; 100 kg ha?1 P and 40 kg ha?1 N in Giresun); 3) lime only (calcium carbonate 94%) (3.0 t ha?1 lime in Samsun; 4.5 t ha?1 lime in Ordu and Giresun); 4) early cut only; 5) soil aeration only; 6) fertilizer+lime; 7) fertilizer+lime+early cut; 8) fertilizer+lime+soil aeration, laid out in a randomized complete block design with four replicates at each location. The highest dry matter (DM) yield of kg ha?1 and crude protein content (%) was obtained from the treatments that included fertilizer. There was no difference in DM production between any of the combination treatments that involved fertilizer and the fertilizer alone treatment. Only lime and aeration applications also increased yield compared to control, but not as much as did any treatment including fertilizer. Crude protein content of the pasture ranged from 13.3 to 18.1% across locations. Nitrogen and phosphorus fertilizer are recommended to improve DM yields and herbage quality for pastures under hazelnut orchards.  相似文献   
4.
A nutrition trial was conducted to investigate the effects of dietary lipid levels and supplemental Ulva meal on growth performance, feed efficiency, nutrient utilization, and body composition of juvenile Nile tilapia, Oreochromis niloticus. Four isonitrogenous (CP 40%) diets containing 0% and 5% Ulva meal were formulated to contain 10% (low-lipid; LL) and 20% (high-lipid; HL) crude lipid. Triplicate groups of fish (~10 g) were fed to apparent satiation three times daily for 16 weeks. Fish fed 5% Ulva meal showed an increased growth performance (P < 0.05) compared with fish fed non-Ulva supplemented diets, irrespective of dietary lipid level. In particular, the incorporation of Ulva meal improved specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER). Feeding fish 5% Ulva meal diets resulted in significantly lower carcass lipid content. The results indicate that 5% inclusion of Ulva meal at both dietary lipid levels improves growth performance, feed efficiency, nutrient utilization, and body composition of Nile tilapia.  相似文献   
5.
This study investigated the effects of dietary humic acid sodium salt on growth performance, haemato‐immunological and physiological responses, and resistance of rainbow trout, Oncorhynchus mykiss to Yersinia ruckeri. The experimental fish were divided into four groups; three of them were fed with humic acid incorporated diets (0.3% H3, 0.6% H6, 1.2% H12) and an additive free basal diet served as the control. Growth performance and haematological parameters of rainbow trout were not affected by humic acid supplemented diets (p > 0.05). However, dietary humic acid especially with 0.6% incorporation significantly increased stomach pepsin, intestinal trypsin and lipase activities p < 0.05. Following 60 days of feeding trial, fish were challenged with Yersinia ruckeri for 20 days. At the end of the challenge period, significantly higher (p < 0.05) survival rates were found in the 6% humic acid group compared to all other experimental treatment. Thus humic acid might replace antibiotics in diets for rainbow trout to control yersiniosis.  相似文献   
6.
Tropical Animal Health and Production - The primary objective of the present study was to assess the effects of vitamin and mineral premix (VMP) withdrawal from the diets 30 and 60&nbsp;days...  相似文献   
7.
Sternbergia candida is an important endemic bulbous plant with beautiful white flowers. It grows on the rock slopes of Baba mountains under Lebanon Cedars in the Mugla province of Turkey with Mediterranean climate. The plant is vulnerable and endangered due to illegal collection for local and foreign flower industry. Fast developing tourism industry and rapid urbanization has also shown negative implications on its population. The study reports three years successful adaptation results of S. candida in the continental climate of Ankara in the Central Anatolia. Four groups of bulbs based on diameter (>2.45, 2.15–2.44, 1.85–2.14 and <1.84 cm) were collected from its natural habitat during March, April and May 1998 and were evaluated for frequency (%) of emergence, mean number of leaves per plant, mean plant height, frequency (%) of flowering, frequency (%) of fruit setting. mean number of bulbs per plant, and mean diameter (cm) of regenerated bulbs. All categories of bulbs could be multiplied and adapted successfully with variable frequency of fruit setting. However, the highest fruit set was recorded from 2.15–2.44 cm April collected bulbs. This indicated that the bulb diameter and collection time had clear implications on multiplication, propagation and adaptation of the plant.  相似文献   
8.
A feeding trial was conducted to investigate the effects of the dietary incorporation of autoclaved and raw (non-autoclaved) Ulva meal on juvenile rainbow trout (Oncorhynchus mykiss) growth, nutrient utilization, body composition, diet digestibility and fatty acid composition. An algae-free control diet and four experimental diets with either 5 or 10 % inclusion levels of raw Ulva meal (5 % = RU5; 10 % = RU10) or autoclaved Ulva meal (5 % = AU5; 10 % = AU10) were formulated. Fish were fed three times daily to apparent satiation for 12 weeks. The growth of fish fed RU10 (final weight = 76.7 ± 3.31 g; SGR = 2.8 ± 0.05 %) diet was significantly higher than that in fish fed the AU10 diet (67.5 ± 1.61 g; SGR = 2.6 ± 0.03 %). The feed conversion ratio (FCR) was significantly better in fish fed the RU10 diet (0.9 ± 0.06) compared with control diet (1.0 ± 0.06). Condition factor, viscerosomatic index and dress-out remained unaffected by dietary treatment. The hepatosomatic index (HSI) was significantly lower (P < 0.05) in fish fed the AU10 diet (1.2 ± 0.19) than fish fed the other diets (≥1.6). The level of eicosapentaenoic acid (20:5n-3—EPA) in muscle from fish fed the RU10, AU5 and AU10 diets was significantly higher than in fish fed control diet (P < 0.05). Dietary inclusion of Ulva meals resulted in a significant increase in muscle docosapentaenoic acid (22:5n-3—DPA) levels at the end of the feeding period. The results indicate that the dietary inclusion of raw Ulva meal at levels of up to 10 % can be used without significant negative effects on the growth performance, nutrient utilization, dietary digestibility and muscle fatty acid composition. In fact, the inclusion of dietary raw and autoclaved Ulva meal elevated n-3 fatty acids levels of rainbow trout muscle.  相似文献   
9.
The present study investigated the effects of dietary trans‐cinnamic acid (TrCin) on growth performance, biochemical composition, fatty acid composition, blood biochemistry, antioxidant status, digestive enzyme and liver antioxidant gene (EF1α, SOD1, SOD2, CAT, GPX1, GPX4, GR and GST) expression responses of rainbow trout, Oncorhynchus mykiss. Five experimental groups of fish with mean weights of 17.49 ± 0.08 g were used in the study; four of them were fed with TrCin‐supplemented diets (0.25 g/kg TrCin25, 0.50 g/kg TrCin50, 0.75 g/kg TrCin75 and 1.50 g/kg TrCin150), whereas an additive‐free basal diet served as the control (Cntr). At the end of the 60‐day feeding trial, the growth performance, biochemical composition and fatty acid composition were similar for all experimental groups. A decrease was observed in intestinal and stomach pH, serum triglyceride and AST, ALT, LDH and ALP levels in fish fed with especially 0.50 g/kg TrCin‐supplemented diet. Moreover, dietary TrCin especially at 0.50 g/kg incorporation level significantly increased the serum SOD and liver SOD2, CAT, GST, GPX1, GPX4 and GR gene expression responses. As a conclusion, feeding rainbow trout for a period of 60 days with a diet containing 0.50 g/kg TrCin might be sufficiently enough to improve the levels of antioxidant enzymes and health status in fish.  相似文献   
10.
Multiple shoot formation from the plumular apices excised from mature embryos of cowpea cv. Akkiz was obtained after pulse treatment with 10 mg/l BAP for 5 days followed by culture on MS medium containing 0.25, 0.50, 0.75 and 1.00, 1.25 mg/l BAP – with or without 0.10 mg/l NAA. Callus induction and shoot regeneration was recorded on all cultures containing BAP with or without NAA. However, inclusion of 0.1 mg/l NAA had positive effect on callus diameter and shoot length. Maximum mean number of 7.11 shoots per explant were obtained on MS medium containing 1.00 mg/l BAP. Longer shoots were recorded on MS medium containing various concentration of BAP+ 0.1 mg/l NAA compared to those containing various concentrations of BAP singly. All shoots cultured on MS medium containing 1 mg/l BAP were rooted on MS medium containing 0.50 mg/l IBA. Rooted plants were acclimatized at room temprature in soil contained in pots. All plants flowered and set seeds in the greenhouse after 3 months.  相似文献   
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