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1.
Standard limb, six lead (I, II, III, aVR, aVL, and aVF) electrocardiograms (ECGs) were recorded in 10 awake mature rock partridges (Alectoris graeca) and 10 chukar partridges (Alectoris chukar). Durations and amplitudes of P and T waves and QRS complexes, durations of P-Q and Q-T intervals, and mean heart rates were calculated from the lead II ECGs. All observable P and T waves were negative in aVR and aVL, whereas they were positive in all remaining leads. The most frequent forms of QRS complex were r-s (r-S) and q-r (q-R). A Q wave was observed in all aVR and aVL leads in both species. Chukar partridges had significantly higher amplitudes of P and T waves and QRS complexes than rock partridges. Mean heart rates were 310+/-15 beats/min and 317+/-19 beats/min for chukar partridges and rock partridges, respectively. Mean electrical axes, calculated from leads II and III, were -99+/-6.3 degrees and -95+/-1.7 degrees for chukar partridges and rock partridges, respectively. Clear ECGs were easily obtainable without anesthesia or sedation.  相似文献   
2.
BackgroundThe microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen.ObjectivesThe present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees.MethodsA procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration.ResultsUR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 104 spores/bee, and the stable detection level was ≥ 2.40 × 105 spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 104 copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min.ConclusionsUR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.  相似文献   
3.
Forty-two horses from Normandy (France) were examined post-mortem for small strongyle infections from October to March. In the positive horses, total worm numbers ranged from 234 to 90,247 (mean 11,297). Encysted larvae represented the major part of the total cyathostome burdens with a high percentage (83%) being early third stage larvae. They were mostly recovered from the caecum (48%) and ventral colon (40%) and were less present in the dorsal colon (12%). Adult cyathostomes were mainly located in the ventral colon (64%) and less frequently in the dorsal colon (27%) and caecum (9%). Twenty species of Cyathostominae were identified. The 10 most prevalent species (in sequence of prevalence) were Cyathostomum coronatum, Cylicocyclus nassatus, Cylicocyclus insigne, Cyathostomum catinatum, Cylicostephanus goldi, Poteriostomum imparidentatum, Cyathostomum labiatum, Cylicocyclus ultrajectinus, Cylicostephanus calicatus and Cylicostephanus minutus which comprised 84% of the total adult population. Twelve species showed a site preference in the ventral colon, five in the dorsal colon and only one in the caecum while two species were collected in nearly equal numbers from the ventral and dorsal colon. The number of species per horse ranged from 1 to 12 with a median of 5. Infections with singletons occurred in 12.5% of the positive horses while multiple infections were encountered in 87.5%. A positive correlation was found between the intensity of cyathostome infection and its diversity, measured either by the number of occurring species or Shanon indexes.  相似文献   
4.
In this study, phytochemical and antioxidant properties of anthocyanin-rich mulberry species of Morus nigra L. (black mulberry) and Morus rubra L. (red mulberry) fruits harvested from across Turkey were investigated. Fruit color, total phenolics (TP), total monomeric anthocyanin (TMA), titratable acidity (TA), and individual sugar and organic acid compositions were determined. Total antioxidant capacity (TAC) of fruits was assessed by both the trolox-equivalent antioxidant capacity (TEAC) and the ferric reducing antioxidant power (FRAP) assays. Black mulberry exhibited higher TP, TMA, TAC and TA when compared to red mulberry. The average TP contents of M. nigra and M. rubra were 2737 and 1603 μg gallic acid equivalent in g fresh weight basis (GAE/g fw), respectively. M. nigra had the richest amount of anthocyanin with an average of 571 μg cy-3-glu/g fw. Overall, TAC averaged 10.5 and 12.0 mmol TE/L by the TEAC and FRAP methods, respectively. We found that FRAP, TEAC, TP and TMA were significantly correlated (r = 0.64–0.99) with each other. Fructose (5.27 g/100 mL) and glucose (5.81 g/100 mL) were determined to be the major sugars in both mulberries. M. nigra displayed a higher TA (2.05 g/100 mL) than M. rubra (0.78 g/100 mL), with citric acid as the major acid.  相似文献   
5.
Sheep are considered as a major contributor of global food security. Moreover, sheep preweaning growth traits as well as in vivo carcass composition traits such as ultrasonic measurements of Longissimus dorsi muscle depth (UMD) and back-fat thickness (UFD) are crucially important indicators of meat yield and hot carcass composition. Despite their relative importance for productivity and profitability of a sheep production system, detected QTL for these traits are quite scarce. Therefore, we implemented GWAS for these traits using animal mixed model-based association approach provided by GenABEL in Esme sheep. Three genome-wide and 14 individual chromosome-wide associated SNPs were discovered. As a result, ESRP1, LOC105613082, ZNF641, DUSP5, TEAD1, SMOX, PTPRT, RALYL, POM121C, PHIP, LOC101106051, ZIM3, PEG3, TRPC7, FBXL4, LOC105610397, LOC105616489 and DNAAF2 were suggested as candidates. Some of the discovered genes and involved pathways were already annotated to contribute growth and development in various species including human, mice and cattle. All in all, the results of this study are expected to strongly contribute to shed a light on the underlying molecular mechanisms behind growth and carcass composition traits, with potential implications on studies aiming faster genetic improvement, targeted low-resolution SNP panel designs and genome-editing studies.  相似文献   
6.
In this article the effect of different nitrogen fertilizer application on the availability of zinc (Zn) content in non calcic brown forest soil was investigated. For this purpose increasing rates of NH4Cl, (NH4)2S04; NH4N03 and Ca(N03)2.4H20 fertilizers were applied for having different physical and chemical properties in two soil samples. An incubation experiment was carried out over 4 months. The available Zn content of the soils was determined each month. According to the results the highest available Zn content of the soils was obtained from NH4Cl applied to the soils followed by (NH4)2S04; NH4N03 and Ca(N03)2.4H20 application to soil. At the end of the experiment the average available Zn content of non calcic brown forest soils for NH4Cl, (NH4)2S04; NH4N03 and Ca(N03)2.4H20 fertilizers are 0.93, 0.91, 0.88 and 0.77 mg kg?1, respectively.  相似文献   
7.
8.
Forests are among the most important ecosystems that provide various hydrological services including water production and protection because the vast majority of the water available on the earth comes from forested catchments. The forests provide quality and continuous water yield as well as protective services such as flood control, soil protection, landslide protection, avalanche protection, and prevention against rock falls, which are related to water. However, the quality and quantity of all the forest functions are influenced by forest ecosystem structure and composition such as forest type, tree species, age class distribution, biomass, leaf area, basal area, crown closure, and stand development stages. This study provides an assessment of hydrological services of forest ecosystems through the systematic review of relevant literature till 2018. It mainly focuses on water-forest relations and explains how forest ecosystem structure and composition affect water production, quality, and sustainability. Then, a general framework relating to the integration of hydrological services into forest management is concluded in the context of sustainable management of natural resources.  相似文献   
9.
Specific components of cranberry fruit are being associated with human health attributes, such as maintenance of urinary tract health and antioxidant status. Some of the chemical properties and antioxidant capacity of cranberry (Vaccinium macrocarpon Ait. cv. Pilgrim) fruits were investigated at light green, blush, light red and dark red maturation stages. Fruit total phenolics, total monomeric anthocyanins, soluble solids, titratable acidity and individual organic acids were examined. Antioxidant capacity of fruits were determined by both the ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays. The fruit color was measured using a portable chromameter. A converse relationship was found between total phenolics and anthocyanin as fruits mature. Total phenolic concentration was declined from 7990 to 4745 mg GAE/kg fw, while total monomeric anthocyanin content was increased from 0.8 to 111.0 mg/kg fw from green to dark red stage. Brix was increased from 6 to 9.3% as well. The main organic acid was citric acid determined by the HPLC method. The antioxidant capacity of cranberries decreased to light red stage; when a fruit accumulates more anthocyanin the activity increased again in both FRAP and TEAC methods. Averaged antioxidant capacity measured was 12.61 and 17.48 mmol TE/kg fw by FRAP and TEAC methods.  相似文献   
10.
The frequencies of neurologic diseases and various corresponding causes were analyzed in 4,319 Norman horses examined by autopsy between 1986 and 2006. Five hundred forty-three cases of nervous system disorders were detected, or a total prevalence of 12.6%. One hundred eighty-seven of the 543 horses (34.4%) exhibited lesions of a traumatic origin affecting the skull or the vertebral column at different levels. Their frequency was less in foals younger than 1 month of age and higher in horses aged 6 months to 2 years. Eighty-three cases of cervical vertebral compressive myelopathy (CVCM) (15.3%) were identified. Males and geldings were more often affected than females. CVCM was diagnosed mostly in animals aged 6 months to 2 years. Horses affected with lesions in the mid-cervical region (region C3–C5) were significantly younger than those exhibiting more caudal lesions (region C5–C7). Neonatal maladjustment syndrome was diagnosed in 62 foals younger than 3.5 days (4.8% of the causes of death between birth and 1 month) by using clinical or lesional criteria. The prevalence was higher in Thoroughbreds as well as in the miscellaneous breed category and less in French Standardbreds. Bacterial meningoencephalomyelitis and meningitis represented 9.6% of nervous system disorders (52 cases) and most often affected foals aged 1 to 6 months. The main bacteria identified were beta-hemolytic Streptococci , Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Actinobacillus sp. The other causes diagnosed were, in decreasing order of frequency: grass sickness or equine dysautonomia (6.3% of neurologic diseases), hepatic encephalopathy syndrome (5.2%), septic vertebral arthritis or osteitis mainly caused by Rhodococcus equi (2.6%), congenital defects of the nervous system (2.4%), equine herpesvirus 1 myeloencephalopathy (2.2%), botulism (2.2%), malformations of the vertebral column other than CVCM (2%), and neighboring polyneuritis associated with guttural pouch mycosis (1.5%).  相似文献   
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