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排序方式: 共有119条查询结果,搜索用时 18 毫秒
1.
牛体外受精胚胎衍生干细胞能力影响因素的研究 总被引:1,自引:0,他引:1
以牛卵巢卵母细胞体外受精获取囊胚期胚胎,比较体外受精牛胚胎不同获取内细胞团的方法和不同培养液对体外培养胚胎干细胞能力的影响。结果表明,囊胚期胚胎不除去透明带而直接培养产生胚胎干细胞,初次克隆率为55%;胰酶法去透明带分离的内细胞团(ICM)培养产生胚胎干细胞,初次克隆率为90%。免疫外科法去透明带分离的ICM在4种不同的培养液中,初次克隆率分别为16.4%、11.5%、21.8%、18.2%,但是其分离的ICM经传代后形成的衍生细胞不易发生分化,经过4~6代的传代,仍然保持其完整的形态,说明免疫外科法是一种理想的牛ICM分离法,培养液为D20+LIF(40ng/mL)可用于牛胚胎干细胞培养。 相似文献
2.
M. Peters S. A. Tarawali R. Schultze-Kraft J. W. Smith A. Musa 《Journal of Agronomy and Crop Science》1999,182(1):25-35
The potential of pastures sown with species mixtures of tropical legume species compared with single species was investigated in subhumid west Africa, over a period of three grazing seasons. Through compensation and complementation of the individual legume species, the mixtures were seasonally and temporally (seasonal refers to different seasons in a year, temporal to different years) more stable than the single species stands. Crude protein contents of individual legumes in the mixtures were higher than of the same species grown in single stands, indicating possible synergistic effects in the mixtures. The ability of legume mixtures to withstand environmental and management variations and, thus, their subsequent potential for on-farm utilization under farmers' conditions are discussed. 相似文献
3.
Ayiguli ABUDUKELIMU LUO Qiu-jiang Musa SHAWUTI Gulinisha Zhayidan ABULIZ HE Ping 《中国畜牧兽医》2015,42(6):1436-1442
In this paper through digestive and metabolic experiments studied the voluntary intake and apparent digestibility of cottonseed hulls (kernel-contained CH,kernel-free CH) and cornstalk on sheep to evaluate the nutrition value and digestion characteristics of cottonseed hulls.12 of adult Xinjiang merino rams were selected and divided into two groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,fed with kernel-free CH by 400 (group 1) or 800 (group 2) g/(head·day) respectively,to estimate the digestibility of CH by difference method;As well,another 18 of adult Xinjiang merino rams were selected and divided into three groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,cafeteria feeding with kernel-contained CH (group 3),kernel-free CH (group 4) or ground cornstalks (group 5) respectively,to study the effects of three diets on the voluntary intake,digestion and metabolism of sheep.The results showed that the apparent digestibility of the DM,OM,CP,cellulose,hemicellulose and energy of kernel-free CH by sheep were 46.71%,47.37%,-53.40%,66.60%,62.48% and 42.59%,respectively.There were no significant differences in the intake of DM,OM,cellulose,hemicellulose,energy and Ca between kernel-contained CH and kernel-free CH groups (P> 0.05);Intake of CP and P in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);Intake of lignin in kernel-contained CH group was extremely significantly lower than that in kernel-free CH group (P< 0.01).There were no significant differences in the apparent digestibility of DM,OM and energy between kernel-contained CH and kernel-free CH groups (P> 0.05).The apparent digestibility of cellulose and hemicellulose in kernel-free CH group were significantly and extremely significantly higher than that in kernel-contained CH group (P< 0.05;P< 0.01),respectively.The apparent digestibility of CP in kernel-contained CH group was extremely significantly higher than that in kernel-free CH group (P< 0.01).The apparent digestibility of DM,OM,CP,cellulose and energy in ground cornstalks group were extremely significantly higher than that in kernel-contained CH and kernel-free CH groups (P< 0.01),however,the apparent digestibility of Ca and P were lower than that in kernel-contained CH and kernel-free CH groups (P> 0.05).Intake and retention of nitrogen in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);The was no significant difference in the retention of nitrogen between kernel-free diet group and cornstalk diet group (P> 0.05).It was concluded that CH was a kind of special forage with larger intake,higher digestibility on the fiber substances and energy,but with a serious obstruction on protein digestion;The nutritional value between kernel-free diet and cornstalk diet was almost equal,and with lower nitrogen digestibility on kernel-free diet. 相似文献
4.
Hadia OM Khair Ibrahim A Adam Shakir B Bushara Kamal H Eltom Nasreen O Musa Imadeldin E Aradaib 《Irish veterinary journal》2014,67(1):4
Background
Bluetongue virus (BTV) is an insect-transmitted virus, which causes bluetongue disease (BT) in sheep and a fatal hemorrhagic infection in North American white-tailed deer. However, in cattle the disease is typically asymptomatic and no overt clinical signs of disease appear to be associated with BTV infection. Serological evidence and isolation of different BTV serotypes have been reported in Sudan, however, no information is currently available in regard to previous exposure of Sudanese livestock to BTV infection in East Darfur State, Sudan.Aims
To determine the prevalence of BTV antibodies and to identify the potential risk factors associated with BTV infection among cattle in East Darfur State, Sudan.Methods
A total of 224 blood samples were collected randomly from five localities in East Darfur State, Sudan. The serum samples were screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA).Results
Serological evidence of BTV infection was observed in 150 out of 224 animals accounting for a 67% prevalence rate among cattle in East Darfur State. Older cattle (>2 years of age) were six times more likely to be infected with BTV (OR = 6.62, CI = 2.87-15.26, p-value = 0.01). Regarding animal source (contact with other herds) as a risk factor, it was shown that cattle purchased from market or introduced from other herds were 3 times at higher risk of being infected with BTV (OR = 3.87, CI = 1.07-13.87, p value = 0.03). Exposure of cattle to the insect vector increased the risk of contracting BTV infection by six times compared to non-exposed cattle (OR = 6.44, CI = 1.53-27.08, p value = 0.01).Conclusion
The present study indicated that age, animal source and the intensity of the insect vector are influential risk factors for BTV infection in cattle in the Darfur region. Surveillance for BTV infection should be extended to include other susceptible ruminants and to study the distribution of the insect vectors to better predict and respond to a possible BTV outbreak in the State of East Darfur, Sudan. 相似文献5.
Sanae El Maaloum Alae Elabed Zainab El Alaoui-Talibi Abdelilah Meddich Abdelkarim Filali-Maltouf Allal Douira 《Communications in Soil Science and Plant Analysis》2020,51(5):622-634
ABSTRACTThe exploitation of phosphate mines generates an important quantity of phosphate sludge that remains accumulated and not valorized. In this context, composting with organic matter and rhizospheric microorganisms offers an interesting alternative and that is more sustainable for agriculture. This work aims to investigate the synergetic effect of arbuscular mycorrhizal fungi (AMF), phosphate-solubilizing bacteria (PSB) and phospho-compost (PC), produced from phosphate-laundered sludge and organic wastes, and their combination on plant growth, phosphorus solubilization and phosphatase activities (alkaline and acid). Inoculated mycorrhizae and bacteria strains used in this study were selected from plant rhizosphere grown on phosphate-laundered sludge. Significant (p < .05) increases in plant growth was observed when inoculated with both consortia and PC (PC+ PSB+ AMF) similar to those recorded in plants amended with chemical fertilizer. Tripartite inoculated tomato had a significantly (p < .05) higher shoot height; shoot and root dry weight, root colonization and available P content, than the control. Co-inoculation with PC and AMF greatly increased alkaline phosphatase activity and the rate of mycorrhizal intensity. We conclude that PC and endophytic AMF and PSB consortia contribute to a tripartite inoculation in tomato seedlings and are coordinately involved in plant growth and phosphorus solubilization. These results open up promising prospects for using formulate phospho-compost enriched with phosphorus-solubilizing microorganisms (PSM) in crop cultivation as biofertilizers to solve problems of phosphate-laundered sludge accumulation. 相似文献
6.
Twenty-one strains of Nocardia farcinica isolated from cattle with bovine farcy in the Sudan were examined to determine their taxonomic relationships. The strains had morphological, cultural and physiological properties of Nocardia, but exhibited some variations of minor taxonomic importance. All strains contained lipids characteristic of nocardiae (LCN-As) and all except two were found by immunodiffusion tests to be serologically related to each other, to N asteroides but not to Mycobacterium tuberculosis nor M bovis. Consequently, these organisms were regarded as true members of the genus Nocardia. 相似文献
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9.
Cihan Ka?ar Kadir ?zcan ?smet Tak?? Kutlay Gürbulak Hasan ?zen Musa Karaman 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):429-431
Congenital malformations with multiple anomalies have been described infrequently in the veterinary literature. A stillborn male crossbred lamb with diprosopus, craniorachischisis, and arthrogryposis was examined macroscopically and histopathologically in this study. The left head was smaller than the right head. Micrencephaly, agnathia, and a rudimentary tongue, which was adherent to the palate, were present in the left head. Micrencephaly, brachygnathia superior, and cleft palate were present in the right head. Cerebellar agenesis and spinal cord hypoplasia were observed. The cerebrums and the spinal cord were covered with a tapering membranous structure. Neural and dermal tissues were noted to intervene upon microscopic examination of this structure. Disorganization of neurons was observed in both cerebrums, though it was more severe in the left one. This case demonstrates many congenital defects occurring together in a lamb. 相似文献