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Deficiency of molybdenum (Mo) in acid soils causes poor growth of pulses. An experiment was, therefore, conducted in greenhouse to study the effect of Mo, phosphorus (P), and lime application on the dry matter yield and plant Mo concentration of lentil (Lens esculenta L.) in two Mo‐deficient acid alluvial soils. The experiment was conducted using a factorial design with three levels of lime (no lime, half, and full lime requirement), three levels of P (0, 25, and 50 mg kg‐1), and two levels of Mo (0 and 1.0 mg kg‐1). Plants were grown for 60 days and at harvest their dry matter yield and Mo concentration were recorded. The three treatments significantly increased dry matter yield, Mo concentration and Mo uptake, the increase being most pronounced with Mo application followed by lime and P. Increases due to applied Mo were greater in presence than in absence of added P; while the reverse was true with the liming treatments. Liming and P application at their lower levels also interacted positively for better Mo nutrition of plants. Results thus indicated that the severity of Mo deficiency in the lentil plants may be reduced by lime and P application in Mo‐deficient acid alluvial soils. 相似文献
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Tadaaki Tokunaga Fortune N. Jomane Shintarou Mandai Takafumi Ishida Hiroyuki Hirooka 《Animal Science Journal》2021,92(1):e13533
The objective of this study was to develop mathematical equations for describing the change in marbling in Japanese Black steers using longitudinal measurements. Serial ultrasound measurements were taken at 14, 16, 20, and 26 months of age and analyzed using an image analysis software. The longitudinal marbling measurements from the ultrasound images and carcasses were fitted into a nonlinear logistic curve. Data used for the analysis consisted of 749 steers that converged in nonlinear curve fitting and showed reasonable estimated parameters of the logistic curves. The average predicted mature beef marbling score (BMS) and maturation rate were 6.26 and 0.353, respectively, and the average maturity levels at 24 months of age were 83.9%. The heritability estimates for the predicted maturity traits were moderate, indicating that these traits may have potential for genetic improvement. There was a negative relationship between the expected progeny differences between carcass BMS and maturity traits, suggesting that genetic improvement by carcass BMS may lead to the selection of bulls with late maturity for marbling. The results indicate that ultrasound and model building for marbling can be useful tools to correctly select candidate bulls with high marbling in the early fattening period. 相似文献
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Background
Virus-induced gene silencing (VIGS) is an effective technology for the analysis of gene functions in plants. Though there are many reports on virus vectors for VIGS in plants, no VIGS vectors available for Rosaceae fruit trees were reported so far. We present an effective VIGS system in apple, pear, and Japanese pear using Apple latent spherical virus (ALSV) vectors.Results
Inoculation of ALSV vectors carrying a partial sequence of endogenous genes from apple [ribulose-1, 5-bisphosphate carboxylase small subunit (rbcS), alpha subunit of chloroplast chaperonin (CPN60a), elongation factor 1 alpha (EF-1a), or actin] to the cotyledons of seeds by a particle bombardment induced highly uniform knock-down phenotypes of each gene on the true leaves of seedlings from 2~3 weeks after inoculation. These silencing phenotypes continued for several months. Northern blot and RT-PCR analyses of leaves infected with ALSV containing a fragment of rbcS gene showed that the levels of rbcS-mRNA drastically decreased in the infected apple and pear leaves, and, in reverse, rbcS-siRNAs were generated in the infected leaves. In addition, some of apple seedlings inoculated with ALSV vector carrying a partial sequence of a TERMINAL FLOWER 1 gene of apple (MdTFL1) showed precocious flowering which is expected as a knock-down phenotype of the silencing of MdTFL1 gene.Conclusions
The ALSV-based VIGS system developed have provides a valuable new addition to the tool box for functional genomics in apple, pear, and Japanese pear. 相似文献4.
从已构建的牛微小隐孢子虫子孢子cDNA文库中,用PCR方法扩增出子孢子表面抗原p23基因,将其插入到编码谷胱肽转移酶(GST)的质粒(pGEX-4T-2)多克隆位点,构建原核表达载体pGEX-p23。重组质粒经EcoRI/Sal I酶切、测序鉴定后转入大肠杆菌BL21中,IPTG诱导表达目的蛋白,SDS-PAGE和Western blot鉴定表达蛋白。结果成功构建了pGEX-p23原核表达载体,经IPTG诱导,转化的大肠杆菌表达出分子质量为47kDa的GST融合蛋白,且该蛋白能与抗p23抗体发生特异性结合反应。本研究为进一步研究p23的生物学功能和临床应用奠定了基础。 相似文献
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