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1.
Akihito TAKAHASHI Ajalli RAHIM Miki TAKEUCHI Emiko FUKUI Midori YOSHIZAWA Kuniaki MUKAI Makoto SUEMATSU Hidetoshi HASUWA Masaru OKABE Hiromichi MATSUMOTO 《The Journal of reproduction and development》2016,62(1):43-49
Tubulointerstitial nephritis antigen-like 1 (Tinagl1, also known as adrenocortical zonation factor 1 [AZ-1]
or lipocalin 7) is a matricellular protein. Previously, we demonstrated that Tinagl1 expression was restricted
to extraembryonic regions during the postimplantation period and detected marked expression in mouse
Reichert’s membranes. In uteri, Tinagl1 is markedly expressed in the decidual endometrium during the
postimplantation period, suggesting that it plays a physical and physiological role in embryo development
and/or decidualization of the uterine endometrium during pregnancy. In the present study, in order to
determine the role of Tinagl1 during embryonic development and pregnancy, we generated
Tinagl1-deficient mice. Although Tinagl1–/– embryos were not
lethal during development to term, homologous matings of Tinagl1–/– females and
Tinagl1–/– males showed impaired fertility during pregnancy, including failure
to carry pregnancy to term and perinatal lethality. To examine ovarian function, ovulation was induced with
equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG); the number of ovulated oocytes did
not differ between Tinagl1–/– and Tinagl1flox/flox.
In vitro fertilization followed by embryo culture also demonstrated the normal
developmental potential of Tinagl1-null embryos during the preimplantation period. Our
results demonstrate that Tinagl1 deficiency affects female mice and results in subfertility phenotypes, and
they suggest that although the potential of Tinagl1–/– oocytes is normal, Tinagl1
is related to fertility in adult females but is not essential for either fertilization or preimplantation
development in vitro. 相似文献
2.
Hitoshi NAKAMURA Yukari UETAKE Masao ARAKAWA Ikuko OKABE Naoyuki MATSUMOTO 《Journal of General Plant Pathology》2001,67(1):37-40
Basidiocarps of the violet root rot fungus, Helicobasidium mompa, are less frequently used for isolation than are mycelial strands and sclerotia even though the basidiocarps are conspicuously
produced at the trunk base of diseased plants. Basidiocarps are also more suitable for storage. This paper describes an improved
method for obtaining pure cultures from basidiocarps using microcentrifuge tubes to facilitate the awkward steps of rinsing
fungal materials under a dissecting microscope.
Received 28 June 2000/ Accepted in revised form 4 August 2000 相似文献
3.
4.
Abe Keita Ishimura Gakushi Baba Shinya Yasui Shota Nakamura Kosuke 《Fisheries Science》2022,88(1):191-202
The spread of coronavirus disease 2019 (COVID-19) and subsequent lockdown measures have impacted economies and industries worldwide. The fisheries industry witnessed a sharp decline in demand and a slump in fish prices due to its dependence on the food service industry. It is important to quantitatively assess those fish species affected most and the extent of the pandemic’s impact on them, to take specific countermeasures. We propose a time-series analysis as an alternative to the current practice of using ad hoc year-on-year comparisons. Although the pandemic makes it difficult to construct a counterfactual approach due to the lack of an appropriate control group, we use time-series forecasting to simulate normal conditions using pre-pandemic data. In Tokyo, the unit price of fish species that were negatively impacted by the food services industry dropped by 12.65% to 14.64%, and by 26.08% to 28.22% after the declaration of a state of emergency. Seasonality, short weekly cycles, and short-term market trends are factors that affect the price of fish. Species-specific impact estimates related to the COVID-19 pandemic can allow policymakers to implement recovery measures in a more targeted and effective manner. The results of our analysis can increase fishers’ and policymakers’ awareness of the usefulness of economic analyses and incentivize them to release data to establish a system to accumulate and analyze data strategically for urgent and appropriate interventions in the fisheries industry.
相似文献5.
6.
Mother–infant bonding is a universal relationship of all mammalian species. Here, we describe the role of reciprocal communication between mother and infant in the formation of bonding for several mammalian species. Mother–infant bond formation is reinforced by various social cues or stimuli, including communicative signals, such as odor and vocalizations, or tactile stimuli. The mother also develops cross‐modal sensory recognition of the infant, during bond formation. Many studies have indicated that the oxytocin neural system plays a pivotal role in bond formation by the mother; however, the underlying neural mechanisms for infants have not yet been clarified. The comparative understanding of cognitive functions of mother and infants may help us understand the biological significance of mother–infant communication in mammalian species. 相似文献
7.
Background
Invasive ecosystem engineers can facilitate their invasions by modifying the physical environment to improve their own performance, but this positive feedback process has rarely been tested empirically except in sessile organisms. The invasive crayfish Procambarus clarkii is an ecosystem engineer that destroys aquatic macrophytes, which provide a physical refuge for animal prey, and this destruction is likely to enhance vulnerability to predators. Using two series of mesocosm experiments, we tested the hypothesis that the invasive crayfish increases its feeding efficiency on animal prey by reducing submerged macrophytes, thus increasing its individual growth rate in a positive density-dependent manner.Results
In the first experiment, increasing crayfish density reduced both macrophytes and animal prey (dragonfly and chironomid larvae) and, importantly, increased the growth rate of individual crayfish, in accordance with our expectation. In the second experiment, we used artificial macrophytes to clarify whether the physical architecture of macrophytes itself protects animal prey and limits crayfish growth rate. Increasing the artificial macrophyte quantity not only increased the survival of animal prey, but also retarded the crayfish growth rate.Conclusions
We conclude that macrophytes strengthen bottom-up control of crayfish, but this effect can be relaxed by increasing the density of crayfish via reduction in macrophytes. This positive feedback process may explain the crayfish outbreaks and regime shifts occasionally observed in invaded freshwater ecosystems.8.
To investigate the reproductive traits and diel activity ofMonochamus saltuarius, 14 pairs of the adults were reared and observed at each of 20°C and 25°C. Both sexes were more active at 25°C than at 20°C
irrespective of the presence or absence of light. A diel change in behavior during 24 h was found on 33% of a total of 48
observations of three age classes of both sexes. No significant difference was found in the mean longevity between sexes or
between the two temperature conditions. The time required for reproductive maturation showed no significant difference within
the same sex when reared at 20°C and 25°C. The mean oviposition period was significantly longer at 25°C (28.8 days) than at
20°C (12.2 days). The mean lifetime fecundity was significantly greater at 25°C than at 20°C. There was no significant difference
in the oviposition ratio (number of eggs deposited/number of oviposition wounds excavated), oviposition rate or hatchability
for females between the two temperature conditions. 相似文献
9.
Masaharu Komatsu Naoki Ichiyama Takashi Kurimoto Shota Takumi Kazuhiro Shiozaki Yasumasa Sugiyama Tatsuhiko Furukawa Seiichi Ando Saki Itonori Hiroaki Saito 《Fisheries Science》2013,79(2):313-320
We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR. 相似文献
10.
ABSTRACTChanges in volatile compounds in dark and ordinary muscle of yellowtail during 2 days of refrigerated storage at 5°C were investigated. Twenty-seven compounds were identified in ordinary muscle and 39 in dark muscle during 2 days of storage at 5°C. Thirteen compounds at Day 0 of storage and 29 compounds at Day 2—such as 2,3-pentadione, 1-penten-3-ol, (Z)-2-penten-1-ol, and (E,Z)-2,4-heptadienal—identified in the dark muscle showed significantly higher values compared to ordinary muscle. Levels of thiobarbituric acid-reactive substances (TBARS) in dark muscle were significantly higher than those in ordinary muscle throughout 2 days of storage period, and a significant increase in TBARS occurred in just dark muscle at Day 2 of storage. In ordinary muscle, viable cells remained at the same order of magnitude as their initial values for 2 days. Eight aldehydes in ordinary muscle and 25 volatile compounds in dark muscle increased significantly without microbial action prior to increase in TBARS during short-term cold storage. Principal component analysis of the volatile compounds in dark and ordinary muscle was able to differentiate between different storage time samples of same muscle type as well as different muscle samples of same storage time. 相似文献