排序方式： 共有10条查询结果，搜索用时 234 毫秒
1
1.
Nagata S Kurosawa M Kuwajima M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):305-307
A new enzyme immunoassay (EIA) for the measurement of furosemide in horse plasma is described. The lower limit of detection of this EIA method was 7.8 ng/ml. The intra-and inter-assay coefficients of variation ranged from 2.5% to 4.9% and 7.5% to 9.8%, respectively. Cross-reactivity with other compounds was not observed. There was a high correlation (r2=0.987) between the high-performance liquid chromatography and EIA results obtained for furosemide concentrations in horse plasma. These results indicate that the newly developed EIA method is useful for the quantitative analysis of furosemide in horse plasma. 相似文献
2.
3.
Keisuke KAWASE Jumpei TOMIYASU Kazuyuki BAN Ryosuke ONO Satoshi ANDO Aya ONO Ran KIMURA Kanako TOMISAWA Motozumi MATSUI Shun-ichi SHIIHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1454
Lions (Panthera leo) breed well under captivity, so contraception has been commonly conducted for population management, leading to a demand for a less invasive and reversible contraceptive approach in lions. In this study, we examined the efficacy of a commercial gonadotropin-releasing hormone vaccine as a method of suppressing reproductive activity in a sexually matured female lion. Under behavioral restraint, the vaccine was injected twice (days 0 and 109). After the initial vaccination, ovarian activity is still observed. After the second vaccination, contraceptive effect was confirmed for 246 days until restart of estrous cycles. We confirmed only a slight swelling around the injection site after the second vaccination. This study may suggest an alternative option for a contraceptive method in lions. 相似文献
4.
5.
6.
Teruaki TOZAKI Aoi OHNUMA Mio KIKUCHI Taichiro ISHIGE Hironaga KAKOI Kei-ichi HIROTA Kanichi KUSANO Shun-ichi NAGATA 《Journal of Equine Science》2021,32(4):125
One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR. 相似文献
7.
Sembon S Fuchimoto D Iwamoto M Suzuki S Onishi A 《The Journal of reproduction and development》2011,57(2):307-311
The aim of the present study was to examine the feasibility of fluorescent in situ hybridization (FISH) for detecting a chromosome 1-specific sequence as a means of assessing the ploidy of porcine parthenotes. In vitro-matured oocytes with the first polar body (PB) were electrically activated; some were treated with cytochalasin B to prevent second PB extrusion (1PB embryos), and the others extruded the second PB (2PB embryos). At the 2-cell stage, one and two FISH signals were detected in each nucleus of 2PB and 1PB embryos, respectively. Almost all cells of blastocysts derived from 1PB embryos retained two signals. In contrast, cells of blastocysts derived from 2PB embryos had two signals. These data demonstrate that FISH analysis allows precise ploidy assessment of porcine parthenogenetic embryos, hence providing a practical means of detecting ploidy transition during parthenogenetic embryogenesis. 相似文献
8.
Shun-ichi Yokota 《Forest Pathology》1983,13(7):389-402
Resistant progenies of Pinus monticola, improved in the northwestern USA, and Asian and European white pines were inoculated with the blister rust fungus, Cronartium ribicola, of Hokkaido. No resistance was observed in P. monticola but the other pines remained healthy. This shows that the resistance of white pines to the blister rust fungus is limited to a particular pathogenic race and a different race of the rust fungus exists in Hokkaido. 相似文献
9.
We investigated an upstream area of the 1926 Taisho Mudflow that occurred at Mount Tokachi, a volcano in central Hokkaido, Japan, to clarify the relationship between natural forest succession and mudflow-induced sediment characteristics. The study area was divided into three parts, i.e., undisturbed, deposition, and scoured areas, based on disturbance regimes. The deposition area was further divided into three different forest stands for a final total of five forest stand types. We assumed that the mudflow regimes created sediment edaphic heterogeneity and undisturbed and island forests supplied seeds for natural revegetation. The undisturbed forest stand comprised pioneer species, whereas a mosaic forest consisting of almost pure stands characterized by Betula ermanii and Picea glehnii developed in the mudflow. This indicates that each plant species has a characteristic ability to establish and adapt initially and later develop into a mosaic forest according to sediment edaphic conditions, particularly depth, grain size distribution, and water and nutrient gradients. The differences in forest species composition and a 30-year time lag between the development of forest stands at the distal edges and the center explain how the cross-sectional sediment edaphic heterogeneity created by the mudflow regimes affected succession and forest development. Furthermore, plants are specific to sediment depth and texture, as well as moisture and nutrient availability, which play important roles in their growth and development; thus, forest stands with contrasting species and age structures developed in the mudflow. 相似文献
10.
1