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Tsuyoshi OZAWA Takashi IJICHI Mitsuya SHIRAISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1786
Microparticles (MPs) are released from budding plasma membranes into body fluids. The use of flow cytometry for the measurement of MP in canines has not been standardized. In this fundamental study, we compared the effect of anticoagulant agents, such as acid-citrate-dextrose (ACD) and heparin on the measurement of canine MPs in platelet-free plasma (PFP) using flow cytometry. In addition, we used annexin V, carboxyfluorescein succinimidyl ester (CFSE), or calcein tetraacetoxymethyl ester (calcein-AM), and explored the characteristics of the staining reagents in MP detection using flow cytometry. We were able to measure canine MPs in PFP prepared from ACD-anticoagulated blood using flow cytometry, in which the highest positive rate for fluorescent staining was observed when CFSE was used. 相似文献
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Fumiko TAGUCHI Rui TANAKA Sayuri KINOSHITA Yuki ICHINOSE Yoshiyuki IMURA Salamah ANDI Kazuhiro TOYODA Tomonori SHIRAISHI Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and
potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using
recombinant harpin indicated harpinpsta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpinpsta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant
of the host-parasite specificity in P. s. pv. tabaci.
Received 10 August 2000/ Accepted in revised form 21 December 2000 相似文献
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Young-Ha SHIN Shunta SHIRAISHI Kiyoaki NARABARA Asaki ABE Yasuhiro KONDO 《Animal Science Journal》2012,83(2):156-161
Estrogen has been reported to act on B cell genesis in the bursa of Fabricius of chick embryos. In this study, we attempted to demonstrate the hypothesis that B cell genesis is controlled by estrogen receptor (ER) in the bursal cells and steroidogenic enzymes synthesized in the bursa. We previously reported the presence of estrogen receptor α (ERα) in the bursa during the late stage of embryogenesis and an increase in the expression of ERα messenger RNA (mRNA) between the 13th day and 16th day. The number of ER-positive cells was maximal on the 16th day. In the present study, ER-positive cells in the bursa during the late stage of embryogenesis increased 4 h after β-estradiol treatment on the 14th to 18th day. The concentration of β-estradiol in the embryonic bursa increased. These results suggest that this stage of embryogenesis is critical in B cell development in the bursa in connection with the effect of estrogen treatment. Our findings also showed that the mRNA expression of five steroidogenic enzymes occurred in the bursa of chick embryos. These results suggest that estrogen is synthesized in the embryonic bursa and estrogen acts on the bursal cells in a paracrine fashion. 相似文献
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D. UENO A. YABUKI T. OBI M. SHIRAISHI A. NISHIO & A. MIYAMOTO 《Journal of veterinary pharmacology and therapeutics》2009,32(3):264-270
We investigated the effect of bradykinin (BK) on isolated equine basilar arterial rings with and without endothelium. BK induced concentration-dependent contraction of resting arterial rings and no relaxation when the rings were precontracted by prostaglandin F2α . The maximal response and pD2 value were 161.2 ± 28.1% (to 60 m m KCl-induced contraction) and 8.24 ± 0.25 respectively. The cumulative concentration–response curve for BK was not shifted to the right by des-Arg9 -[Leu8 ]-BK (a B1 -receptor antagonist), HOE140 (a B2 -receptor antagonist) or NPC567 (another B2 -receptor antagonist). In four of six basilar arteries, NPC567 induced concentration-dependent contraction. Indomethacin (a cyclooxygenase inhibitor), nordihydroguaiaretic acid (a lipoxygenase inhibitor), quinacrine (a phospholipase A2 inhibitor), tetrodotoxin (a selective blocker of Na+ channels), guanethidine (a nor-adrenergic neuron blocking drug), phentolamine (an α-adrenoceptor antagonist), Nω-nitro- l -arginine ( l -NNA, a nitric oxide (NO) synthase inhibitor) and endothelial denudation did not affect the BK-induced contraction. l -NNA and indomethacin induced contraction and relaxation under resting vascular tone respectively. These results suggest that endothelial cells are not involved in BK-induced contraction and that the contraction is not mediated via activation of known B1 and B2 receptors. Arachidonic acid metabolites and neurotransmitters like norepinephrine and NO might not play a role in BK-induced contraction in equine basilar artery. 相似文献
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SAYAKA SHIRAISHI IZUMI WATANABE KATSUJI KUNO YOSHIHARU FUJII 《Weed Biology and Management》2002,2(3):133-142
The effects of leaches from dry leaves of 71 ground cover plant species on lettuce were tested at the first screening. The inhibitory effects on radicle and hypocotyl elongations of lettuce varied with the different species of cover plants that were used. Eight species of Oxalis showed strong inhibitions (4–27% of untreated control on radicle elongation). Inhibitory activities of seven species of cover plants on three weed species, live amaranth ( Amaranthus lividus ), southern crabgrass ( Digitaria ciliaris ) and common lambsquarters ( Chenopodium album ), were tested at the second screening. Moss pink ( Phlox subulata ), trefoil ( Oxalis brasiliensis ), red spiderlily ( Lycoris radiata ), creeping thyme ( Thymus serpyllum ), European pennyroyal ( Mentha pulegium ), roman chamomile ( Chamaemelum nobile ) and star-of-Bethlehem ( Ornithogalum umbellatum ) were selected as donor plants because of their high inhibitory effects on lettuce growth and their usefulness as ornamental ground cover plants. Effects of leaches from dry leaves and exudates from the roots of these species were assayed on agar. Radicle elongations of all tested weed species were inhibited by leaches from trefoil and red spiderlily (8–31% and 14–24% of untreated control, respectively) and exudates from moss pink, trefoil and creeping thyme (11–43%, 31–74% and 22–67% of untreated control, respectively). 相似文献
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SAYAKA SHIRAISHI IZUMI WATANABE KATSUJI KUNO YOSHIHARU FUJII 《Weed Biology and Management》2005,5(3):128-136
Laboratory and field experiments were conducted to evaluate the usefulness of Oxalis spp. as allelopathic ground-cover plants for weed management. Some Oxalis spp. have previously been reported to possess strong allelopathic activities but few studies have been conducted on their activities in fields. This study aimed to investigate allelopathic activities and the possibility of weed suppression in five species of common Oxalis : shamrock oxalis ( Oxalis articulata Savigny), Bowie's woodsorrel ( Oxalis bowiei Lindl.), trefoil ( Oxalis brasiliensis Lodd. ex Knowl. et West.), lucky clover ( Oxalis deppei Lodd. ex Sweet) and Oxalis hirta L. The effects of the leachates from dry leaves and the exudates from living roots of these plant species were tested in laboratory experiments. The leachates from O. articulata , O. bowiei , O. deppei and O. hirta and the exudates from O. deppei caused > 84% inhibition of the radicle elongation of lettuce seedlings, but no effect was observed on the seed germination of lettuce. In the field experiment, O. deppei significantly reduced the weed population in July. A significant relationship was observed between the weed population and the percentage ground coverage of Oxalis spp. In contrast to the weed population, a significant relationship was observed between the weed above-ground biomass and the allelopathic activity of exudates from Oxalis spp. 相似文献
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Yoshiyuki IMURA Satoko IGUCHI Kazuhiro TOYODA Yuki ICHINOSE Tomonori SHIRAISHI Tetsuji YAMADA 《Journal of General Plant Pathology》2000,66(2):123-127
Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea (PSPAL1) in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions
in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness,
we constructed Box-I-deletion PSPAL1 promoter (dB-1) with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of
Box-I for PSPAL1 activation induced by nonpathogenic attack.
Received 27 October 1999/ Accepted in revised form 25 November 1999 相似文献