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Farzaneh Assadian Gholamreza Nikbakht Siavash Niazi Reza Farahani Khaltabadi Monireh Jahantigh 《Veterinary research communications》2014,38(2):157-163
The eukaryotic expression plasmid of M2e-Hsp70 is a candidate M2e-based DNA vaccine. In order to evaluate the immunization potential of this construct, Specific Pathogen Free chickens were immunized either intramuscularly or orally. Mutant Salmonella typhimurium was used as carrier for oral delivery of the M2e-Hsp70 construct. M2e-specific humoral and cellular responses were tested by ELISA and Lymphocyte Proliferation Assay, respectively. Our results indicate that both humoral and cellular immune responses are conferred against M2e-Hsp70 plasmid in either of the intramuscular or oral routes of administration; however, these responses are significantly higher in intramuscular injection in contrast to oral administration. When it comes to mass vaccination of commercial chicken flocks oral administration is preferred due to the ease of application as well as its capability of eliciting mucosal, humoral and cellular immune responses; so measurements should be taken to improve the immunization potency of our orally delivered DNA vaccine. 相似文献
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Attempts to phosphorylate myo-inositol with various polyphosphates or urea-phosphate under aqueous conditions proved unsuccessful, despite reports to the contrary. The easiest way to phosphorylate myo-inositol completely is to heat it with H3PO4 under reduced pressure at 150°C for 6 h. 相似文献
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B. H. Niazi J. Rozema R. A. Broekman M. Salim 《Journal of Agronomy and Crop Science》2000,184(2):101-110
A pot experiment was conducted under greenhouse conditions with two subspecies of beet, fodderbeet ( Beta vulgaris cv. Majoral) and seabeet ( Beta maritima ), under saline conditions. Growth and physiological parameters (dry weight, leaf area, water relations and net photosynthesis) were recorded. The two subspecies responded differently in terms of growth parameters. Plant growth was significantly reduced at 400 m m NaCl, while no significant growth reduction occurred at 200 m m NaCl. Fairly high values of relative growth rate were associated with the leaf area of the cultivars. The water content of the shoot decreased with plant age. The fodderbeet cultivar Majoral showed a significant increase in dry matter at 200 m m NaCl after 5 weeks. This growth improvement may be related to the better adaptation of the plants under saline conditions. 相似文献
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Abstract Seed soak treatment of sunnhemp (Crotalaria juncea L.) with the systemic pesticides, benomyl, methomyl, phorate and carbofuran, gave protection against infection with tobacco mosaic virus (TMV). These chemicals significantly inhibited infection on sunnhemp plants and reduced virus infectivity, as well as delaying the appearance of systemic symptoms. Benomyl was found to be the most effective chemical. 相似文献
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Alleviation of Salinity Stress by Vermicompost Extract: A Comparative Study on Five Fennel Landraces
Abdollah Beykkhormizi Siavash Hosseini Sarghein Mohammad Reza Sarafraz Ardakani Seyed Mohammad Moshtaghioun Seyed Mousa Mousavi Kouhi 《Communications in Soil Science and Plant Analysis》2018,49(17):2123-2130
In the present study, interaction between salinity (0, 40, 80, 120 mM NaCl) and vermicompost extract (VCE) on five fennel landraces (Urmia, Mashhad, Shiraz, Boushehr, Isfahan) was investigated in a factorial experiment as completely randomized design to find the best salt-tolerant landrace and potential alleviating role of VCE. Results showed that Boushehr and Isfahan were the most tolerant and sensitive landrace to salinity, respectively. Application of VCE improved germination and growth of salt-treated fennels. The amylase activity of fennels was not affected by salinity and not improved by VCE. While sodium (Na+) content of root and shoot of all salt-treated fennels increased, their potassium (K) content decreased. Under salinity stress, root K content of VCE-amended landraces was more than that of not amended ones. VCE also caused an increase in calcium (Ca) content of root of salt-treated Shiraz and Urmia landraces. In conclusion, VCE can alleviate adverse effects of salinity stress on fennel. 相似文献
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Marina Rafiq Muhammad Shahid Saliha Shamshad Sana Khalid Nabeel Khan Niazi Ghulam Abbas Muhammad Farhan Saeed Mazhar Ali Behzad Murtaza 《Journal of Soils and Sediments》2018,18(6):2271-2281
Purpose
This study delineated the effect of calcium (Ca) and ethylenediaminetetraacetic acid (EDTA) addition on arsenic (As) accumulation and physiological attributes of Vicia faba L.Materials and methods
Two separate experiments were performed. In the first experiment, V. faba seedlings, grown under hydroponic conditions, were exposed to three levels of As (25, 125 and 250 μM) in the presence and absence of three levels of EDTA (25, 125, 250 μM) and calcium (CaCl2: 1, 5 and 10 mM). The effect of EDTA and Ca on As accumulation and physiological attributes of V. faba was assessed by determining As contents in roots and shoot, chlorophyll contents, H2O2 contents, and lipid peroxidation in young and old leaves. In the second experiment, V. faba seeds were grown in As-contaminated sand culture using the same treatment plan.Results and discussion
The accumulation and toxicity of As to V. faba plants increased with increasing As levels in nutrient solution. Arsenic exposure enhanced the production of reactive oxygen species (ROS) in both roots and leaves, which resulted in lipid peroxidation and decreased chlorophyll contents. The presence of both EDTA and Ca, in general, significantly decreased As accumulation by V. faba seedlings, Ca being more effective than EDTA. Both the amendments decreased As-induced reactive oxygen species (ROS) production and lipid peroxidation. In the case of chlorophyll contents, EDTA significantly decreased chlorophyll contents, while Ca significantly increased chlorophyll contents compared to As. The effect of all the treatments was more pronounced in roots than leaves and in young leaves compared to old leaves.Conclusions
It is proposed that EDTA and Ca greatly affect As accumulation and toxicity to V. faba, and the effect varies greatly with their applied levels as well as type and age of plant organs. The germinating seedlings of V. faba may be preferred for risk assessment studies, while transplanting 1-week-old V. faba seedlings to As-contaminated soils can decrease its toxicity.9.
Induced polyploidy in inter‐subspecific maize hybrids to reduce heterosis breakdown and restore reproductive fertility 
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下载免费PDF全文 I. A. K. Niazi S. Rauf J. A. Teixeira da Silva Z. Iqbal H. Munir 《Grass and Forage Science》2015,70(4):682-694
The aim of this study was to reduce the breakdown of hybrid vigour by crossing maize × teosinte subspecies through induced tetraploidy and to improve the biomass and reproductive fertility of the induced tetraploids. To achieve this, seeds of maize breeding lines selected from an open‐pollinated population ‘Sargodha‐2002’ and a maize × teosinte cross were germinated in colchicine solution (0·25, 0·5 or 1·0%) until they had a thick radical and protruded plumule. Colchicine at 0·5% induced the highest number of tetraploids with the lowest number of chimeric plants. The induced tetraploids of maize and maize × teosinte crosses showed a significant (P ≤ 0·05) increase in leaf area (5 and 14%), total soluble solids (20 and 18%), leaf oil percentage (42 and 12%) and leaf crude protein contents (10 and 14%) in leaves relative to the diploid subspecies. Moreover, induced tetraploidy also arrested hybrid vigour by slowing down the decay of heterosis in progenies (4x) for plant height, leaf biomass and leaf oil percentage. Induced maize × teosinte hybrid tetraploids selected for frequent bivalent chromosome pairing resulted in higher seed setting cob?1 (increased 58%), pollen fertility (increased 59%) and seed setting percentage (increased 100%) than the quadrivalent variant. 相似文献
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【目的】比较不同来源葡萄果实中叶酸积累的差异,并利用分子标记技术对高叶酸种质资源进行评价和筛选。【方法】采用ELISA技术检测22份葡萄种质资源果实叶酸含量;利用ISSR分子标记对22份葡萄种质资源进行基因组多态性分析和聚类分析;分析检测出叶酸含量与聚类结果。【结果】22份葡萄种质资源果实叶酸含量的范围在1.93～46.95 ng/g,在遗传相似系数为0.621处,22份葡萄材料明显分为2大类群。第1大类叶酸含量平均为35.79 ng/g;其余为第2大类,叶酸含量平均为15.71 ng/g。在遗传相似系数为0.622处,21份葡萄材料又明显分为2大类群。第1大类叶酸含量平均为16.53 ng/g;第2大类叶酸含量平均为12.24 ng/g。欧亚种的叶酸含量较欧美杂种稍高3.998 7 ng/g;从30条引物中筛选出9条扩增稳定且多态性丰富的引物用于葡萄的ISSR扩增。共扩增出78条条带,其中多态性条带70条,多态性百分率为89.7%。22份葡萄种质资源的遗传相似系数为0.474 4～0.846 2,平均遗传相似系数为0.654 5。在遗传相似系数为0.621处,22份葡萄材料明显分为2... 相似文献