Early harvest and ensilage of forage sorghum infected with ergot (Claviceps africana) reduces the risk of livestock poisoning     

BJ Blaney  MJ Ryley  BD Boucher 《Australian veterinary journal》2010,88(8):311-312

Sorghum ergot produces dihydroergosine (DHES) and related alkaloids, which cause hyperthermia in cattle. Proportions of infected panicles (grain heads), leaves and stems were determined in two forage sorghum crops extensively infected 2 to 4 weeks prior to sampling and the panicles were assayed for DHES. Composite samples from each crop, plus a third grain variety crop, were coarsely chopped and half of each sealed in plastic buckets for 6 weeks to simulate ensilation. The worst-infected panicles contained up to 55 mg DHES/kg, but dilution reduced average concentrations of DHES in crops to approximately 1 mg/kg, a relatively safe level for cattle. Ensilation significantly (P = 0.043) reduced mean DHES concentrations from 0.85 to 0.46 mg/kg.  相似文献   

Quantitative structure-activity relationship modeling of peptide and protein behavior as a function of amino acid composition     

Siebert KJ 《Journal of agricultural and food chemistry》2001,49(2):851-858

A quantitative structure-activity relationship (QSAR) modeling approach based on the location of each amino acid along three axes obtained by principal component analysis (called z scores) was extended to physical and functional properties of proteins, where the proportion of particular amino acids rather than a precise sequence is the determining factor. Coomassie Brilliant Blue spectral responses to amino acid homopolymers (R = 0.926) and proteins, either as a function of their contents of six basic and aromatic amino acids (R = 0.976) or as a function of the contributions of these amino acids to the three z scores (R = 0.935), were modeled. The ultraviolet absorbance of proteins was modeled in terms of the z score contributions of tyrosine, tryptophan, and cysteine (R = 0.995). Modeling many protein functional properties in this manner appears to be possible. An approach to modeling peptide behaviors that depend on short sequences of amino acids was also considered.  相似文献   

Pyogranulomatous myocarditis due to Staphylococcus aureus septicaemia in two harbour porpoises (Phocoena phocoena)     

Siebert U  Müller G  Desportes G  Weiss R  Hansen K  Baumgärtner W 《The Veterinary record》2002,150(9):273-277

Staphylococcus aureus septicaemia was diagnosed in a dead, stranded harbour porpoise from the German Baltic Sea and in a live harbour porpoise by-caught in inner Danish waters and taken into captivity. Lesions included pyogranulomatous myocarditis, necrotising suppurative bronchopneumonia, pyelonephritis, osteomyelitis and leptomeningitis, and abscesses in lymph nodes and skeletal muscles. The captive animal had fibrinous suppurative epicarditis and pyogranulomatous myocarditis with abscesses. In both animals the organism was suspected to have entered through skin lesions or via the respiratory tract.  相似文献   

Acid-base parameters in blood, erythrocytes, liver tissue and urine in decompensated metabolic acidosis of cattle     

G Lachmann  H Siebert  M Sch?fer 《Archiv fuer experimentelle veterinaermedizin》1985,39(3):422-428

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Intraerythrocytic acid-base status in decompensated metabolic acidosis and with parenteral buffer administration     

G Lachmann  H Siebert  M Sch?fer 《Archiv fuer experimentelle veterinaermedizin》1985,39(4):598-605

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Cryptosporidium infection in a dugong (Dugong dugon)     

BD HILL  IR FRASER  HC PRIOR 《Australian veterinary journal》1997,75(9):670-671

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Effects of protein-polyphenol interactions on beverage haze, stabilization, and analysis   总被引：14，自引：0，他引：14  

Siebert KJ 《Journal of agricultural and food chemistry》1999,47(2):353-362

The haze-forming activity of a polypeptide depends greatly on its proline content. Haze-forming polyphenols have at least two binding groups, each of which has at least two hydroxy groups on an aromatic ring. The protein/polyphenol ratio has a strong influence on the amount of haze formed; the largest amount occurs when the numbers of polyphenol binding ends and protein binding sites are nearly equal. This has important consequences for turbidimetric methods used to measure haze-active proteins and polyphenols in beverages. The ratio also influences the effectiveness of a number of stabilization procedures.  相似文献   

Modeling protein functional properties from amino acid composition     

Siebert KJ 《Journal of agricultural and food chemistry》2003,51(26):7792-7797

Physicochemical and functional properties of proteins were modeled as a function of the contributions of each of the 20 coded amino acids to three (z-scores) or five (extended z-scores) amino acid principal properties using partial least squares regression. The five term models were in all cases stronger in both fit and prediction than the three term models, indicating that useful information is contained in the fourth and fifth property scores. Models predicting protein hydrophobicity (R = 0.932), viscosity (R = 0.737), and foam capacity (R = 0.880) from amino acid composition rather than sequence were obtained. It is likely that additional functional and physicochemical properties of proteins can be modeled in this way.  相似文献   

Prediction of herbage intake and liveweight gain of cattle grazing tropical pastures from the composition of the diet     

B.D. Siebert  R.A. Hunter 《Agricultural Systems》1977,2(3):199-208

As a basis for calculating growth rates of cattle within a model of a beef herd, three experiments were carried out. Their purpose was to (i) develop equations to predict organic matter, digestible organic matter and metabolisable energy intakes of cattle, (ii) establish the conversion of metabolisable energy to live weight of tropical cattle, and (iii) establish the validity of these relationships for predicting the liveweight change of grazing animals.In the first experiment, Brahman × Shorthorn crossbred cattle were held either indoors in pens or in long laneways outdoors where they walked approximately 5 km per day. Organic matter intake was measured in both situations and was found to be not significantly different between the situations. Regression equations were calculated relating intake of organic matter and digestible organic matter by cattle indoors to the chemical composition of their diet. The conversion of digestible organic matter to metabolisable energy intake and finally to liveweight gain was confirmed in a second experiment. The liveweight gain of outdoor animals was predicted accurately provided the metabolism of crossbred animals was considered and an allowance made for the energy required for walking.The diet selected by oesophageally fistulated animals at pasture was chemically analysed over a four-month period following these measurements. Feed intake and liveweight gain of non-fistulated animals grazing native and improved pastures were predicted from the chemical composition of the diet selected. There was good prediction of liveweight gain in both pasture situations.  相似文献   

Acute interstitial pneumonia in cattle associated with consumption of mould-damaged sweet potatoes (Ipomoea batatas)     

BD HILL  HF WRIGHT 《Australian veterinary journal》1992,69(2):36-37

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