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1.
Kobayashi H Pohjanvirta T Pelkonen S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1071-1073
The role of birds as sources of Shiga toxin-and intimin-producing Escherichia coli was studied. Fecal samples from live gulls (n=86), pigeons (n=33) and broiler chickens (n=199) from 23 flocks were analyzed for stx and eae by PCR. No stx positive samples were detected. In contrast, eae E. coli were highly prevalent among gulls (40%), and was also found in pigeons (7%) and chickens (57% of the flocks contaminated). The eae positive isolates were analyzed genetically and O-serogrouped. One isolate from a pigeon was found to have stx (2f). The isolates of gulls differed from those of pigeons and chickens, and all eae E. coli isolates from birds differed from human pathogenic strains by the lack of EHEC-hlyA and bfp/EAF as well as distribution of O-serogroups. Thus, birds cannot be regarded as important carriers of zoonotic stx or eae E. coli in Finland. 相似文献
2.
Sinikka Mynttinen Kalle Karttunen Tapio Ranta 《Scandinavian Journal of Forest Research》2014,29(1):41-50
The consumption of energy wood in Finland has increased dramatically since the early 2000s, and the region of South Savo is a bellwether for its usage across the country. This increase is partly due to the national policy of sustainable energy, region's wealth of forests but is also dependent on non-industrial private forest owners (NIPF) deciding to sell energy wood. In this study, a survey with a structured questionnaire was conducted among the NIPFs in the region of South Savo to enlarge the understanding of their willingness to sell energy wood in the future and adopt new approaches to forestry that involves energy wood thinning as well as their current production of energy wood. Moreover, the factors influencing forest owner's motivations relating to energy wood thinning as well as information needs were analysed with multivariate techniques. The results show that almost half of the forest owners in the South Savo region had not sold energy wood. However, forest owners within the region generally had positive attitudes towards selling small-sized energy wood and forestry methods that involve energy wood thinning. Moreover, forest owners' willingness to sell different types of energy wood varied, depending on their background. The study suggests that the increase in supply of energy wood presumes both intensified extension services for forest owners and markets that are better organised than at the moment. The results may also prove beneficial in informing policy-makers regarding which specific target group(s) to focus on when promoting energy wood sales among NIPFs and which aspects of energy wood harvesting and sales to promote. 相似文献
3.
Variation in the hyphal growth rate and the virulence of two genotypes of the crayfish plague organism Aphanomyces astaci 
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下载免费PDF全文 S Viljamaa‐Dirks S Heinikainen A‐M K Virtala H Torssonen S Pelkonen 《Journal of fish diseases》2016,39(6):753-764
Crayfish plague, a devastating disease of freshwater crayfish, is caused by an oomycete organism, Aphanomyces astaci. Currently five genotypes of A. astaci are known, but variable features between the strains or genotypes have not been studied extensively. This study analysed 28 isolates of the As genotype and 25 isolates of the Ps1 genotype and reveals that the radial growth rate is significantly (P < 0.001) different between these two genotypes, although highly variable inside the genotype As. Two Ps1 genotype isolates and two As genotype isolates with different radial growth rates were tested in an infection trial. Clear differences were detected in the development of mortality in the test groups. The representatives of the Ps1 genotype caused total mortality within a short time span. The As genotype isolates were much less virulent. The slow‐growing As isolate showed higher virulence than the As isolate with a high growth capacity. Although slow growth could be one survival strategy of the pathogen, several other mechanisms are involved in the pathogenicity and warrant further studies. 相似文献
4.
Fossil M Ahlsten K Pohjanvirta T Anttila M Kokkonen T Jensen TK Boye M Sukura A Pelkola K Pelkonen S 《Acta veterinaria Scandinavica》2005,46(4):257-267
A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilities at the age of 11 days. At 46 days, 8 pigs were inoculated with B. pilosicolihipp- strain Br1622, 8 pigs were inoculated with B. pilosicoli type strain P43/6/78 and 7 pigs were sham-inoculated. No signs of spirochaetal diarrhoea were detected; only one pig, inoculated with P43/6/78, had soft faeces from day 9 to 10 post inoculation. The pigs were necropsied between days 7 and 23 after inoculation. Live pigs were culture-negative for Brachyspira spp., but B. pilosicolihipp- was reisolated from necropsy samples of two pigs. The lesions on large colons were minor and did not significantly differ between the three trial groups. In silver-stained sections, invasive spirochaetes were detected in colonic mucosae of several pigs in all groups. Fluorescent in situ hybridisation for genus Brachyspira, B. pilosicoli and strain Br1622 was negative. However, in situ detection for members of the genus Leptospira was positive for spirochaete-like bacteria in the colonic epithelium of several pigs in both infected groups as well as in the control group. L. intracellularis, Salmonella spp., Yersinia spp. and intestinal parasites were not detected. The failure of B. pilosicoli strains to cause diarrhoea is discussed with respect to infectivity of the challenge strains, absence of certain intestinal pathogens and feed and management factors. 相似文献
5.
Willow buffer strips are a promising vegetated filter for the reduction of non-point source pollution from agricultural land
to watercourses resulting from the rapid growth of shoots and from the large amount and distribution of roots. A greenhouse
experiment tested the hypothesis that a new planting method, planting cuttings horizontally, exhibited more biomass production
and more effective filter than did planting cuttings vertically. Due to the different times taken for shoots break through
the soil surface, the summed height of all shoots of horizontally planted cuttings was significantly smaller than that of
the vertical ones at the beginning of the growth period. The difference in the height of all of the shoots between planting
orientations decreased with time. After 16 weeks growth, the biomass of the stem, leaves and fine roots was not affected by
the planting orientation. The coarse root biomass was larger in the horizontal treatments and decreased with increased density.
The total biomass, as well as the biomass of stem or leaves, of each treatment, increased with increased planting density.
The individual plant biomass, as well as that of the stem or leaves, decreased with increased planting density. Compared with
the unplanted control treatment, the planted treatments significantly reduced the total-N, NO3-N, PO4-P and SO4-S leaching. The planting orientation did not affect the nitrate leaching. The horizontally planted cuttings were slightly
more effective for reducing the SO4-S leaching and the vertically planted cuttings were slightly more effective for reducing the PO4-P leaching. Lower PO4-P leaching was observed only with higher planting density. With regard to the horizontal planting method, further studies
are needed to explore the influence of different willow clones, the size of cuttings, pre-planting treatments, planting geometry
(configuration) and soil conditions on survival, the number of shoots produced, the biomass production and the amount and
distribution of roots. 相似文献
6.
Ulrike Lyhs Ilona Ikonen Tarja Pohjanvirta Kaisa Raninen P?ivikki Perko-M?kel? Sinikka Pelkonen 《Acta veterinaria Scandinavica》2012,54(1):64
Background
Extraintestinal pathogenic Escherichia coli bacteria (ExPEC) exist as commensals in the human intestines and can infect extraintestinal sites and cause septicemia. The transfer of ExPEC from poultry to humans and the role of poultry meat as a source of ExPEC in human disease have been discussed previously. The aim of the present study was to provide insight into the properties of ExPEC in poultry meat products on the Finnish retail market with special attention to their prevalence, virulence and phylogenetic profiles. Furthermore, the isolates were screened for possible ESBL producers and their resistance to nalidixic acid and ciprofloxacin was tested.Methods
The presence of ExPEC in 219 marinated and non-marinated raw poultry meat products from retail shops has been analyzed. One E. coli strain per product was analyzed further for phylogenetic groups and possession of ten virulence genes associated with ExPEC bacteria (kpsMT K1, ibeA, astA, iss, irp2, papC, iucD, tsh, vat and cva/cv) using PCR methods. The E. coli strains were also screened phenotypically for the production of extended-spectrum β-lactamase (ESBL) and the susceptibility of 48 potential ExPEC isolates for nalidixic acid and ciprofloxacin was tested.Results
E. coli was isolated from 207 (94.5%) of 219 poultry meat products. The most common phylogenetic groups were D (50.7%), A (37.7%), and B2 (7.7%). Based on virulence factor gene PCR, 23.2% of the strains were classified as ExPEC. Two ExPEC strains (1%) belonged to [O1] B2 svg+ (specific for virulent subgroup) group, which has been implicated in multiple forms of ExPEC disease. None of the ExPEC strains was resistant to ciprofloxacin or cephalosporins. One isolate (2.1%) showed resistance to nalidixic acid.Conclusions
Potential ExPEC bacteria were found in 22% of marinated and non-marinated poultry meat products on the Finnish retail market and 0.9% were contaminated with E. coli [O1] B2 svg+ group. Marinades did not have an effect on the survival of ExPEC as strains from marinated and non-marinated meat products were equally often classified as ExPEC. Poultry meat products on the Finnish retail market may have zoonotic potential. 相似文献7.
T Kaipainen T Pohjanvirta N Y Shpigel A Shwimmer S Py?r?l? S Pelkonen 《Veterinary microbiology》2002,85(1):37-46
Escherichia coli isolates from bovine mastitis were examined for a selection of virulence factors. The strains originated from Finland and Israel, which have differences in the proportion of mastitis caused by E. coli, clinical pictures of coliform mastitis, environmental conditions and herd management. The genes of nine virulence factors were detected by polymerase chain reaction. Presence of K1 and K5 capsules was assessed by use of specific bacteriophages. Serum resistance was tested by a turbidimetric assay. Out of 160 Finnish isolates, 37% had traT, 14% cnf2, 8% cnf1, 11% aer, 9% f17, 8% sfa, 7% pap, 1% afa8D and 1% afa8E. Out of 113 Israeli isolates, 41% had traT, 4% aer, 3% cnf2, 1% cnf1, 1% sfa and 1% f17. Some of the genes were distributed among two major pathotype groups, with either f17 family or sfa, pap and cnf1 as major determinants. Genes for F17a, CS31A, Afa7D and Afa7E were not detected. Altogether 49% of Finnish and 42% of Israeli isolates had at least one virulence gene, but genes other than traT were present in only 24% of Finnish and 5% of Israeli isolates. Serum resistance was more common among Finnish (94/160) than Israeli isolates (19/113). K1 and K5 capsules were not detected. 相似文献
8.
9.
Hybrids of Shigatoxigenic and Enterotoxigenic Escherichia coli (STEC/ETEC) Among Human and Animal Isolates in Finland 下载免费PDF全文
下载免费PDF全文 O. Nyholm S. Heinikainen S. Pelkonen S. Hallanvuo K. Haukka A. Siitonen 《Zoonoses and public health》2015,62(7):518-524
Diarrhoeagenic Escherichia coli (DEC) cause serious foodborne infections in humans. Total of 450 Shigatoxigenic E. coli (STEC) strains isolated from humans, animals and environment in Finland were examined by multiplex PCR targeting the virulence genes of various DEC pathogroups simultaneously. One per cent (3/291) of the human STEC and 14% (22/159) of the animal and environmental STEC had genes typically present in enterotoxigenic E. coli (ETEC). The strains possessed genes encoding both Shiga toxin 1 and/or 2 (stx1 and/or stx2) and ETEC‐specific heat‐stable (ST) enterotoxin Ia (estIa). The identified stx subtypes were stx1a, stx1c, stx2a, stx2d and stx2g. The three human STEC/ETEC strains were isolated from the patients with haemolytic uraemic syndrome and diarrhoea and from an asymptomatic carrier. The animal STEC/ETEC strains were isolated from cattle and moose. The human and animal STEC/ETEC strains belonged to 11 serotypes, of which O2:H27, O15:H16, O101:H‐, O128:H8 and O141:H8 have previously been described to be associated with human disease. Identification of multiple virulence genes offers further information for assessing the virulence potential of STEC and other DEC. The emergence of novel hybrid pathogens should be taken into account in the patient care and epidemiological surveillance. 相似文献
10.
Composting municipal wastewater sludge may generate composting wastewater (acid washer water and tunnel wastewater) with high ammonium–nitrogen (NH4–N) concentration; this kind of wastewater is usually generated in a rather small daily amount. A procedure of air stripping with catalytic oxidation was developed and tested with pilot-scale and full-scale units for synthetic disposal of the high NH4–N wastewaters from composting facilities. In air stripping, around 90% NH4–N removal efficiency was reliably achieved with a maximum of 98%. A model to describe the stripping process efficiency was constructed, which can be used for process optimization. After catalytic oxidation, the concentrations in the outlet gas were acceptable for NH3, NOX, NO2, and N2O, but the NH3 and N2O concentrations limited the feasible loading range. The treatment costs were estimated in detail. The results indicate that air stripping with the catalytic oxidation process can be applied for wastewater treatment in composting facilities. 相似文献