Serotyping of Haemophilus paragallinarum isolates from Mexico by the Kume hemagglutinin scheme     

Soriano VE  Blackall PJ  Dabo SM  Téllez G  García-Delgado GA  Fernández RP 《Avian diseases》2001,45(3):680-683

A total of 42 isolates of Haemophilus paragallinarum from Mexico were serotyped by the Kume hemagglutinin scheme. Serovars A-1, A-2, B-1, and C-2 were recognized among 11 (26.2%), 7 (16.6%), 4 (9.5%), and 14 (33.3%) isolates, respectively. A further six isolates (14.3%) showed hemagglutinating activity but could not be classified into any serovar. Commercial vaccines containing Kume serovars A-1, A-2, B-1, and C-2 may provide better protection than those bi- or trivalent infectious coryza vaccines currently used in Mexico.  相似文献   

Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes          下载免费PDF全文

HH Shiomi  RO Pinho  DMA Lima  JB Siqueira  MCR Santos  EV Costa  PS Lopes  SEF Guimarães  JD Guimarães 《Reproduction in domestic animals》2015,50(4):545-553

This study aimed to assess the effects of different cooling curves and centrifugation regimes used in cryopreservation protocols on the post‐thaw viability of Piau‐breed wild boar (Sus scrofa) sperm using in vitro assessment tests. Two centrifugations (800  g for 10 min and 2400  g for 3 min) and two cooling curves (conventional cooling using nitrogen vapour – freezing 1 and automated cooling using a programmed freezing machine – freezing 2) were tested. Therefore, the treatments were divided into M3 – centrifugation at 2400  g for 3 min and freezing 2; M10 – centrifugation at 800  g for 10 min and freezing 2; R3 – centrifugation at 2400  g for 3 min and freezing 1; and R10 – centrifugation at 800  g for 10 min and freezing 1. No significant differences (p > 0.05) between treatments occurred post‐thawing regarding the total sperm motility means recorded. The mean values of the different treatments were not different from each other regarding the supravital staining (SV), hypo‐osmotic test (HO), sperm–egg binding assay or sperm morphology. This study showed that both the cooling curve and the centrifugation regime affected the quality of post‐thaw sperm, and centrifugation for shorter times and cooling curves using automated cooling are the most suitable for minimizing sperm injury.  相似文献   

Role of Macrophyte Typha latifolia in a Constructed Wetland for Wastewater Treatment and Assessment of Its Potential as a Biomass Fuel     

M.P. Ciria  M.L. Solano  P. Soriano 《Biosystems Engineering》2005,92(4):35

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Soil properties in organic olive groves compared with that in natural areas in a mountainous landscape in southern Spain   总被引：1，自引：0，他引：1  

S. Álvarez    M. A. Soriano    B. B. Landa  & J. A. Gómez 《Soil Use and Management》2007,23(4):404-416

This study evaluates soil properties in organically managed olive groves and natural zones in a mountainous area of Andalusia, Spain. Two soil types (Eutric Regosol and Eutric Cambisol) and the most common soil management methods (tillage and two intensities of grazing) were studied. Both soil types in the groves had values not much lower than those in the natural areas. Average (±SE) values in the groves were 1.58 ± 0.71% for organic carbon, 323 ± 98 g kg^?1 for macroaggregate stability, 1.11 ± 0.16 g cm^?3 for bulk density, 3.5 ± 1.6 mm h^?1 for saturated hydraulic conductivity and 1209 ± 716 mg CO₂ kg^?1 for soil respiration. Overall, these values tended to be lower in the tilled compared with that in the grazed groves. The average phosphorus soil content (5.83 ± 5.22 mg kg^?1) was low for olive production and within adequate ranges for N (0.12 ± 0.05%) and K (142 ± 81 mg kg^?1). Soil erosion was high in the tilled groves (35.5 ± 18.2 t ha^?1 year^?1) with soil loss correlating with indicators of soil degradation such as organic carbon content and water stable macroaggregates. In the grazed groves, soil loss was moderate with no clear indications of soil degradation. Overall, there was significant farm‐to‐farm variability within the same soil and land management systems. Olive production had a moderate effect on soil degradation compared with natural areas and olive cultivation could be sustained in future if cover crop soil management replaced tillage, especially in the most sloping areas.  相似文献   

Sequence interpretation. Functional annotation of mouse genome sequences     

Nadeau JH  Balling R  Barsh G  Beier D  Brown SD  Bucan M  Camper S  Carlson G  Copeland N  Eppig J  Fletcher C  Frankel WN  Ganten D  Goldowitz D  Goodnow C  Guenet JL  Hicks G  Hrabe de Angelis M  Jackson I  Jacob HJ  Jenkins N  Johnson D  Justice M  Kay S  Kingsley D  Lehrach H  Magnuson T  Meisler M  Poustka A  Rinchik EM  Rossant J  Russell LB  Schimenti J  Shiroishi T  Skarnes WC  Soriano P  Stanford W  Takahashi JS  Wurst W  Zimmer A;International Mouse Mutagenesis Consortium 《Science (New York, N.Y.)》2001,291(5507):1251-1255

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The causes of piping in a set of abandoned agricultural terraces in southeast Spain   总被引：1，自引：0，他引：1  

A. Romero Díaz  P. Marín Sanleandro  A. Snchez Soriano  F. Belmonte Serrato  H. Faulkner 《CATENA》2007,69(3):282-293

This study aims to identify some of the factors contributing to the piping process in abandoned terraces in seven sites located in the Murcia Region (southeast Spain). The local landscape has the appearance of badland geomorphology, possessing deep valleys which until the 1970s were terraced and cultivated. The terraces have now been abandoned to the piping process. The research focuses on the relationship between the size and position of pipes and the physical and geochemistry properties of the local highly dispersive marl lithology; and the extent to which the reworking of the materials for terrace cultivation has exacerbated piping erosion.Morphologically, the terracing in the stream beds has resulted in a series of gentle benches separated by steeper risers with variable ‘drop’, (riser height). A series of flat infiltration surfaces result, and because the resculpting produces a reduced bulk density infill with enhanced throughflow, infiltrating water is forced along steep hydraulic gradients to a low outfall at the base of the riser.Paralleling studies of land levelling and terrace abandonment in similar materials elsewhere, piping in the study area is shown to be closely related to the physical and chemical characteristics of the materials its sodicity and dispersive character. Considerable differences in structure, texture, mineralogical composition and material's surface crust and subsoil geochemical properties emerged from ‘site signature’ plots, and these results suggest that agricultural processes affect the dispersive character of the crust materials, presumably because agricultural activity increases the buffering effect of organic acids on the dispersed clay. This is supported by the observation that on the one site that was levelled but not used for agriculture, a stable organically-distinct crust like the abandoned agricultural sites has not formed. The changes to the material's aggregate stability and bulk density properties when terracing occurs is examined, but insufficient evidence is available from the data collected to unpick the causes of aggregate stability changes in the study plots. The way in which differences between crust and subsoil enhance infiltration is discussed, and the role of the terrace ‘drop’ in influencing hydraulic gradients and pipe development is illustrated. A possible index to describe the relationship between evacuated material volumes and the controlling parameters discussed above is tested.It is concluded that terracing which is intended as a conservation practice has, in dispersive materials, actually enhanced piping, leading to the abandonment of farming.  相似文献   

Determination of phytohormones of environmental impact by capillary zone electrophoresis     

Segura Carretero A  Cruces-Blanco C  Soriano Peña M  Cortacero Ramírez S  Fernández Gutiérrez A 《Journal of agricultural and food chemistry》2004,52(6):1419-1422

A test mixture of five phytohormones [naphthaleneacetic acid (NAA), naphthoxyacetic acid (NOA), indoleacetic acid (IAA), indolebutyric acid (IBA), and indolepropionic acid (IPA)] was investigated. These compounds were cleanly separated with good resolution by capillary zone electrophoresis with a UV diode array detector using 20 mM sodium phosphate buffer (pH 7.25). The lowest detection limit was obtained for IPA (0.45 mg L(-)(1) or 0.005 mg kg(-)(1)) and the highest for NAA (1.04 mg L(-)(1) or 0.014 mg kg(-)(1)). The method has been applied for tomato samples fortified with the five phytohormones using a liquid-liquid extraction procedure, obtaining recovery percentages ranging from 91 to 109.0%.  相似文献   

The Neocortex of the Dog   总被引：1，自引：0，他引：1  

I. Salazar    P. Ruiz  Pesini  P. Fdez.  Troconiz  J. Gonzalez  Soriano P. Fdez.  Alvarez 《Anatomia, histologia, embryologia》1988,17(2):169-187

This article describes the structural organization of the neocortex of the dog from purely morphological point of view. Series of 50 urn transverse sections were cut from the frozen right hemiencephala of adult greyhounds, stained by N issl's method, and drawn. His's method was then used to construct life-size lateral, dorsal and medial views of the hemiencephalon from 38 sections located at regular intervals. Once the cytoarchitectonic criteria to be used had been established on the basis of preliminary observations, all the sections of each hemiencephalon were examined microscopically and the cytoarchitectonic types observed in each mapped on the corresponding drawing. These maps were then projected onto lateral, dorsal and medial views of the whole hemiencephalon to produce a classical cytoarchitectonic map of the neocortex made up of 21 types, 7 of them agranular (with no inner granular layer), 2 dysgranular (with a granular layer with diffuse boundans) and the rest granular (with a well-defined layer IV). Various areas of cortex whose characteristics were intermediate between those of adjoinin areas were classified in a residual typus intermedius.  相似文献   

Letter to the Editor     

Oscar Cortadellas  Ma Josefa Fernández del Palacio  Alejandro Bayón  Angel Albert Soriano  Jesús Talavera López  DVM  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(2):225-225

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Effect of temperature on lutein esterification and lutein stability in wheat grain     

Fauziah Tufail Ahmad  Robert E. Asenstorfer  Imelda R. Soriano  Daryl J. Mares 《Journal of Cereal Science》2013

The creamy colour of many wheat-based end-products is conferred by endogenous lutein. During post-harvest storage of grain, free lutein may be converted in part to potentially more stable lutein mono- and di-fatty acid esters. This study investigates the synthesis of lutein esters and stability of free lutein and lutein esters over a wide range of temperatures in grain of a high lutein bread wheat, Triticum aestivum L. line DM5685*B12 and a durum, Triticum durum L cv Kamilaroi. Disappearance of free lutein and lutein esterification followed first order reaction rates. The maximum rate of lutein esterification was at ≈80 °C, however, the optimum temperature for maximum synthesis with minimum degradation was between 30 °C and 60 °C. No ester synthesis was observed at temperatures higher than 120 °C. The data are consistent with an enzyme participating in the esterification reaction. Lutein esters were found to be more stable than free lutein with a longer shelf life at 60 °C whilst at temperatures ≤40 °C, lutein degradation was minimal. This study provides new information on lutein ester formation and lutein stability that should be useful to grain handlers and food manufacturers seeking to optimise retention of lutein for the benefit of consumers.  相似文献