Diagnostic accuracy of optical coherence tomography for assessing surgical margins of canine soft tissue sarcomas in observers of different specialties     

Josephine A. Dornbusch  Laura E. Selmic  Pin‐Chieh Huang  Jonathan P. Samuelson  Eric M. McLaughlin  Vincent A. Wavreille  Jessica A. Ogden  Brittany Abrams  Alex Kalamaras  Eric Green  Eric T. Hostnik  Lincoln Every  Jason A. Fuerst  Ryan Jennings  Christopher Premanandan  Joshua N. Lorbach  Sarah C. Linn  Aneesh Alex  Janet E. Sorrells  Lingxiao Yang  Stephen A. Boppart 《Veterinary surgery : VS》2021,50(1):111-120

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Carcinogen in rock fern (Chielanthes sieberi) from New Zealand and Australia     

BL SMITH  PP EMBLING  DR LAUREN  MP AGNEW  AD ROSS  PL GREENTREE 《Australian veterinary journal》1989,66(7):230-231

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Experimental infection of normal and immunosuppressed pigs with Pseudomonas pseudomallei   总被引：1，自引：0，他引：1  

AD THOMAS  JC FORBES-FAULKNER  TL D''ARCY  JH NORTON  D HOFFMANN 《Australian veterinary journal》1990,67(2):43-46

A single dose of 5 x 10(8) bacilli of Pseudomonas pseudomallei by intratracheal injection resulted in acute (21 cases) or chronic (19 cases) melioidosis in 40 of 48 pigs. Fifteen (10 acute and 5 chronic) had been immunosuppressed by cyclophosphamide before inoculation. The major clinical signs were initial fever, marked neutrophilia and, in the acute cases, respiratory distress. There were no signs of the nasal and ocular discharge, paresis or diarrhoea seen in acute cases in south-east Asia. The cyclophosphamide treatment caused a significant decrease in the neutrophil count by 7 d after inoculation in all 15 immunosuppressed pigs, and all were culture positive at necropsy. Eight of the 33 non-treated pigs were culture negative at necropsy. Pigs overcoming the initial phase of infection had more abscess-like nodules that were bacteriologically sterile at necropsy than the pigs with acute cases of melioidosis. P. pseudomallei was isolated predominantly from the spleen, lungs and the injection site. Although only one strain was used in this study, it is likely that Australian strains of P. pseudomallei are not as virulent as the south-east Asian isolates.  相似文献   

Lymphocystis disease in captive barramundi     

M PEARCE  JD HUMPHREY  AD HYATT  LM WILLIAMS 《Australian veterinary journal》1990,67(4):144-145

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Precision of genetic relationship estimates based on molecular markers   总被引：3，自引：0，他引：3  

José F. Barbosa-Neto  Carlos M. Hernández  Louise S. O’Donoughue  Mark E. Sorrells 《Euphytica》1997,98(1-2):59-67

Genetic progress through selection is directly related to the amount of variability present in the population and the quality of genes contributed by the parents. Genetic relationships between lines were studied using DNA marker-based estimates of genetic similarity. A statistical methodology using the width of a confidence interval was developed to determine the number of probes to be surveyed and the precision in the estimation of genetic distance between pairs of cultivars. Precision was affected by type of genetic distance used, the number of cultivars, and amount of genetic diversity present in the studied group. The width of a (1-α)% confidence interval decreased as the number of RFLP fragments increased. Oat and wheat diversity studies were used to illustrate the methodology. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Distribution of microsatellite alleles linked to Rht8 dwarfing gene in wheat     

M. Ahmad  Mark E. Sorrells 《Euphytica》2002,123(2):235-240

A wheat microsatellite locus, Xgwm 261, whose 192-bp allele closelylinked to the dwarfing gene Rht8, on chromosome 2D, was used toscreen 71 wheat cultivars from 13 countries to assess the variation at thislocus. Screening of this wheat collection showed that a 165-bp allele anda 174-bp allele were the most frequent. None of the New Zealand cultivarspossessed a 192-bp allele specific to Rht8, while only one cultivarfrom the US produced this important allele. The frequency of a 192-bpallele among these wheat cultivars was 5.63%. The highest allelefrequency was observed for a 174-bp fragment (52.11%) followed by a165-bp fragment (26.76%). The only durum wheat `Cham 1', did notshow any amplification due to the absence of D genome. Four new novelalleles, 180-bp, 198-bp, 200-bp and 204-bp present in the US and NewZealand wheat cultivars are reported.  相似文献   

Phytochemicals and antioxidant activity of milled fractions of different wheat varieties   总被引：7，自引：0，他引：7  

Adom KK  Sorrells ME  Liu RH 《Journal of agricultural and food chemistry》2005,53(6):2297-2306

The health-promoting effects of whole-grain consumption have been attributed in part to their unique phytochemical contents and profiles that complement those found in fruits and vegetables. Wheat is an important component of the human diet; however, little is known about the phytochemical profiles and total antioxidant activities of milled fractions of different wheat varieties. The objectives of this study were to investigate the distribution of phytochemicals (total phenolics, flavonoids, ferulic acid, and carotenoids) and to determine hydrophilic and lipophilic antioxidant activity in milled fractions (endosperm and bran/germ) of three different wheat varieties, two of which were grown in two environments. Grain samples of each of the wheat varieties were milled into endosperm and bran/germ fractions. Each fraction was extracted and analyzed for total phenolics, ferulic acid, flavonoids, carotenoid contents, and hydrophilic and lipophilic antioxidant activities. Total phenolic content of bran/germ fractions (2867-3120 micromol of gallic acid equiv/100 g) was 15-18-fold higher (p < 0.01) than that of respective endosperm fractions. Ferulic acid content ranged from 1005 to 1130 micromol/100 g in bran/germ fractions and from 15 to 21 micromol/100 g in the endosperm fractions. The bran/germ fraction flavonoid content was 740-940 micromol of catechin equiv/100 g. On average, bran/germ fractions of wheat had 4-fold more lutein, 12-fold more zeaxanthin, and 2-fold more beta-cryptoxanthin than the endosperm fractions. Hydrophilic antioxidant activity of bran/germ samples (7.1-16.4 micromol of vitamin C equiv/g) was 13-27-fold higher than that of the respective endosperm samples. Similarly, lipophilic antioxidant activity was 28-89-fold higher in the bran/germ fractions (1785-4669 nmol of vitamin E equiv/g). Hydrophilic antioxidant activity contribution to the total antioxidant activity (hydrophilic + lipophilic) was >80%. In whole-wheat flour, the bran/germ fraction contributed 83% of the total phenolic content, 79% of the total flavonoid content, 51% of the total lutein, 78% of the total zeaxanthin, 42% of the total beta-cryptoxanthin, 85% of the total hydrophilic antioxidant activity, and 94% of the total lipophilic antioxidant activity. Our results showed that different milled fractions of wheat have different profiles of both hydrophilic and lipophilic phytochemicals. These findings provide information necessary for evaluating contributions to good health and disease prevention from whole-wheat consumption.  相似文献   

Breeding tef [Eragrostis tef (Zucc.) trotter]: conventional and molecular approaches     

M. Zeid  G. Belay  H. Tefera  M. E. Sorrells 《Plant Breeding》2011,130(1):1-9

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Mutant Trp repressors with new DNA-binding specificities   总被引：16，自引：0，他引：16  

S Bass  V Sorrells  P Youderian 《Science (New York, N.Y.)》1988,242(4876):240-245

Oligonucleotide-directed mutagenesis of the codons for glutamine-68 (Gln68), lysine-72 (Lys72), isoleucine-79 (Ile79), alanine-80 (Ala80), and threonine-81 (Thr81) of the Escherichia coli trpR (tryptophan aporepressor) gene was used to make mutant repressors with each of 36 different amino acid changes. Mutant repressors were tested for binding to each member of a set of 28 different operators closely related to the consensus trp operator. Of the 36 mutant repressors, 11 bind a subset of the 28 operators; 5 of these have new binding specificities. These new specificities indicate that the hydroxyl group of Thr81 makes a specific contact with one of the four critical base pairs in a trp operator half-site, and the methyl group of Thr81 determines specificity at a second, critical base pair. The Trp repressor does not use the first two amino acids of its "recognition alpha-helix," Ile79 and Ala80, to make sequence-specific DNA contacts, and interacts with its operator in vivo in a way fundamentally different from the way that phage lambda repressor, lambda Cro protein, and coliphage 434 repressor contact their respective binding sites.  相似文献   

Spread of epizootic haematopoietic necrosis virus (EHNV) in redfin perch (Perca fluviatilis) in southern Australia     

RJ WHITTINGTON  C. KEARNS  AD HYATT  S. HENGSTBERGER  T. RUTZOU 《Australian veterinary journal》1996,73(3):112-114

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