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Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758) 
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下载免费PDF全文 GL Lima VB Luz LF Lima RMP Rocha SV Castro TS Castelo APR Rodrigues JR Figueiredo AR Silva 《Reproduction in domestic animals》2018,53(4):880-888
The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions. 相似文献
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Roncada P Tomasi L Stracciari GL Ermini L Strocchia A 《Journal of veterinary pharmacology and therapeutics》2000,23(4):237-241
The excretion rate of dicloxacillin from milk was studied after intramammary administration of a suspension of the drug active in vegetable oil. Eight cows and eight sheep, four of each group in low and four in high milk production, were dosed with 200 mg dicloxacillin/quarter in cows and 100 mg dicloxacillin/quarter in sheep, three times at 12 h intervals. The dicloxacillin concentrations in milk were quantified by high performance liquid chromatography (HPLC). In cows, time until dicloxacillin was undetectable was 48 h and no difference was observed between the groups. In sheep, dicloxacillin was undetectable 72 h and 84 h after the treatment in low and in high milk production groups, respectively. The implications of several factors affecting the possible milk withdrawal period were studied. The results indicated that the pharmaceutical vehicle and the coefficient of lipid solubility exerted major effects on depletion time. 相似文献
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Berti A Bottini G Gandola M Pia L Smania N Stracciari A Castiglioni I Vallar G Paulesu E 《Science (New York, N.Y.)》2005,309(5733):488-491
In everyday life, the successful monitoring of behavior requires continuous updating of the effectiveness of motor acts; one crucial step is becoming aware of the movements one is performing. We studied the anatomical distribution of lesions in right-brain-damaged hemiplegic patients, who obstinately denied their motor impairment, claiming that they could move their paralyzed limbs. Denial was associated with lesions in areas related to the programming of motor acts, particularly Brodmann's premotor areas 6 and 44, motor area 4, and the somatosensory cortex. This association suggests that monitoring systems may be implemented within the same cortical network that is responsible for the primary function that has to be monitored. 相似文献
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Gian Luigi Stracciari 《Veterinary research communications》1979,3(1):57-63
The reactivity of bovine foetal intestine to some acetylcholine derivatives is compared with the response to acetylcholine. Qualitatively similar responses are observed between the bovine intestine after mid-foetal life and intestinal tissue from adult animals. A lower reactivity of the foetal preparations could be partly explained by the existence of different levels of cholinesterase in the tissues at different times during embryogenesis. Foetal intestine reacts with different intensity to the cholinergic agents tested.
Kurzfassung Man studiert die Reaktion des Fötusdarmes von Rind auf einige Azethylcholin Derivate im Vergleich mit Azethylcholin. Das Studium erklärt dass der nach der Mitte des embryonalen Lebens Rinddarm und der von erwachsenen Tieren Darm, auf gleiche Weise sich betragen. Eine kleinere Reaktion der Fötuspräparate möchte mit der Anwesenheit von verschiedenen Cholinesterase Konzentrationen in den Geweben während der embryonalen Entwicklung, teilweise erklärt werden. Der Fötusdarm reagiert auf die cholinergischen geprüften Mittel mit verschiedener Intensität.
Resume L'A. étude la réactivité de l'intestin foetal de boeuf à quelques dérivés de l'acétylcholine en comparaison du médiateur cholinergique. Qualitativement des réponses similaires ont été observées dans l'intestin aprés la moitié de la vie foetale et celui des animaux adults. La plus petite réactivité des préparations foetales pourrait être expliquée partiellement par l'existence de concentrations différentes de cholinestérase tissulaire dans de différentes périodes du dévelopement embryonnaire. L'intestin foetal réagit avec diverse intensité aux médicaments cholinergiques éxaminés.
Riassunto L'A. studia la reattività dell'intestino fetale bovino ad alcuni derivati dell'acetilcolina paragonandola con la risposta evocata dal mediatore colinergico. Qualitativamente vengono riscontrate risposte simili tra l'intestino bovino proveniente da feti che hanno superato la metà della vita embrionale e quello di animali adulti. La minore reattività dei preparati fetali potrebbe essere parzialmente interpretata dal reperimento di differenti concentrazioni di colinesterasi tessutali a tempi diversi di sviluppo embrionale. L'intestino fetale reagisce con diversa intensità agli agenti colinergici saggiati.相似文献
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John BUCKERIDGE Tom&#x; KOÍ Jn SCHL
GL Adam TOMA&#x;OVÝCH Martina KOOV VESELSK 《Integrative zoology》2019,14(6):561-575
Fossil cephalopods are frequently encrusted by epibionts; however, determining whether encrustation occurred prior to or post‐mortem to the host, and whether the final environment of deposition corresponds to the habitat of encrustation is complex. The present paper describes cirripede epibionts, their calcareous bases and their attachment scars on 6 post‐mortem shells of Nautilus macromphalus, collected from deep water off New Caledonia. The cirripedes have left both cemented calcareous bases of Hexelasma and scars associated with bioerosion and discoloration produced by verrucomorph barnacles. Live cirripedes included a Metaverruca recta, with articulated opercular plates and organic tissue (on a shell that had been exposed on the sea floor for at least 150 years), and specimens of Hexelasma velutinum, one of which was partly attached to an internal surface of a shell. The disposition of verrucomorphs indicates that most Nautilus shells were colonized post‐mortem rather than during a floating stage. However, as cirripedes are known to have colonized living Nautilus, some Hexelasma, preserved only as calcareous eroded bases, may represent specimens that settled on a living Nautilus. The degree of bioerosion and discoloration induced by verrucomorph barnacles varies according to the surface preservation of Nautilus shells, with deeper and discolored traces preserved on old and degraded shells. Traces made by verrucomorphs described here are ellipsoidal and a new ichnotaxon, Anellusichnus ellipticus, is proposed to accommodate them. Importantly, verrucomorphs and other cirripede taxa with membranous bases that were attached to pristine shells may not leave any substantial scars, and, thus, will be difficult to detect in the fossil record. 相似文献
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