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The seroprevalence of Toxoplasma gondii, Dirofilaria immitis (heartworm), feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections was examined using serum or plasma samples from 746 pet cats collected between May and July 2009 from clinics and hospitals located in and around Bangkok, Thailand. The samples were tested for heartworm, FIV, and FeLV using a commercial ELISA. Of the 746 samples, 4.6% (34/746) were positive for heartworm antigen, 24.5% (183/746) had circulating FeLV antigen, and 20.1% (150/746) had antibodies against FIV. In addition, the first 348 submitted samples were tested for T. gondii antibodies using a modified agglutination test (MAT, cut off 1:25); 10.1% (35/348) were seropositive. Of the 348 cats sampled for all four pathogens, 11, 10, and 1 were positive for T. gondii antibodies and FIV antibodies, FeLV antigen, or D. immitis antigen, respectively. Of the 35 T. gondii-seropositive cats, 42.9% (15/35) were co-infected with at least one of the other three pathogens. The presence of antibodies to FIV was significantly associated with both age and gender, while FeLV antigen presence was only associated with age. In the case of FIV, males were twice as likely to be infected as females, and cats over 10 years of age were 13.5 times more likely to be infected than cats less than 1 year of age. FeLV antigen was more common in younger cats, with cats over 10 years of age being 10 times less likely to be FeLV positive than cats under 1 year of age. This is the first survey for these four pathogens affecting feline health in Thailand.  相似文献   
2.
Ancylostoma ceylanicum is a common zoonotic hookworm of dogs and cats throughout Asia and has also been reported to occur within the Australasian region. The aim of this study to was to determine the efficacy of a spot-on formulation containing emodepside and praziquantel (Profender®, Bayer) and praziquantel and pyrantel oral tablets (Drontal® for Cats, Bayer) against experimental A. ceylanicum infections in cats.Twenty-four kittens were each subcutaneously injected with 100 infective third-stage larvae of A. ceylanicum. Kittens were stratified by egg count and randomly allocated equally into control and two treatment groups. The first group were treated with emodepside 2.1%/praziquantel 8.6% (Profender®, Bayer) at the recommended label dose. The second group was treated with 80 mg pyrantel and 20 mg praziquantel (Drontal® for Cats, Bayer) at the recommended label dose. The kittens in the control group were not treated. Egg counts were performed daily until the end of the study period and compared for the treated and control groups. No eggs were detected in the treated group of kittens within 4 days of treatment and faecal samples from this group remained negative throughout the rest of the study, resulting in a treatment efficacy (egg reduction) of 100% (P < 0.0001). The egg counts remained high (993 ± 666 epg) in the untreated control group for the rest of the study period. This study demonstrated that both combination products containing topical emodepside/praziquantel (Profender®, Bayer) and praziquantel/pyrantel oral tablets (Drontal® for Cats, Bayer) given at the recommended dose is highly effective against infection with A. ceylanicum in cats.  相似文献   
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Lymphatic filariasis has been targeted by the World Health Organization (WHO) to be eliminated by the year 2020. In addition to chemotherapy and vector control, the control of reservoir hosts is necessary for the control program to succeed. Malayan filariasis, caused by Brugia malayi, is endemic in the South of Thailand where domestic cats serve as the major reservoir host. However, in nature, domestic cats also carry B. pahangi, Dirofilaria immitis and D. repens infections and it is difficult to distinguish the different filarial species from each other just by morphology. To assess the burden of filarial parasites, we performed a study on domestic cats in an endemic area of malayan filariasis in the Prasang district, of Surat Thani, a province in Southern Thailand. Together with Giemsa staining and acid phosphatase activity studies, we performed PCR-RFLP analysis on the first internal transcribed spacer (ITS1) region of ribosomal DNA (rDNA). PCR-RFLP with Ase I could clearly differentiate between B. malayi, B. pahangi, D. immitis and D. repens. Out of the 52 cats studied, filarial parasites were identified in 5 (9.5%) cats, of which 4 (7.6%) were B. pahangi and 1 (1.9%) D. immitis. This PCR-RFLP technique detected two additional cats that were not detected by microscopy. The domestic cats are not an important host of B. malayi in this region. We could develop the PCR-RFLP assay test for differentiating filarial nematodes which can be applied to survey human, animal reservoir hosts and mosquito vectors in endemic areas.  相似文献   
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