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1.
AIM: To compare, in cows treated with an internal teat sealant, the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts (SCC) after calving.METHODS: Cows from a spring-calving, pasture-based dairy farm in the Manawatu-Whanganui region of New Zealand were randomly allocated to receive either a short-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=291) or a long-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=288) at the end of lactation. Cows were managed on-farm with routine husbandry procedures through the dry period and following calving. A multivariable logistic regression model was used to determine the association between length of action of dry-cow therapy and the proportion of cows with a SCC >150,000?cells/mL at the first herd test after calving.RESULTS: Age of cow, mean SCC for the preceding season and interval from calving to the first post-calving herd test were all associated with the proportion of cows with an individual SCC >150,000?cells/mL at the first herd test (p<0.001) Treatment with the short-acting dry-cow therapy was not associated with decreased odds of cows having a SCC >150,000?cells/mL at the first herd test compared with treatment with long-acting dry-cow therapy (OR=0.724; 95% CI=0.40–1.30).CONCLUSIONS AND CLINICAL RELEVANCE: In this herd, which routinely used internal teat sealants, the use of short-acting cloxacillin-based dry-cow therapy did not result in an increased proportion of cows with elevated SSC post-calving. This was a single farm, single year study but indicates that in this herd, changing from a long-acting to a short-acting antimicrobial may have no impact on the prevalence of subclinical mastitis. 相似文献
2.
Gene expression in black tiger prawns (Penaeus monodon) was studied following intra-muscular injection of CMVGal plasmid into the second abdominal segment. We used an in situ staining technique to detect -gal expression in one- and three-month-old injected prawns. We found that only one of the three-month-old prawns expressed the marker gene (2 days after injection), and the site of expression was confined to the sixth abdominal segment away from the injection site. We repeated the experiment on a new batch of three-month-old prawns, but using fluorometric determination technique. This time we found that -gal expression was detected (6/42) at the site of injection after 2, 7, and 14 days. In two other test samples, transgene expression was detected in the sixth abdominal segment only, further confirming the possibility of injected DNA dispersal. The results of the study also suggest that direct gene transfer is a feasible technique in black tiger prawns. 相似文献
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Molecular and morphological characterization of stunt nematodes of wheat,maize, and rice in the savannahs of northern Nigeria
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Sulaiman ABDULSALAM PENG Huan LIU Shi-ming HUANG Wen-kun KONG Ling-an PENG De-liang 《农业科学学报》2022,21(2):586-595
Stunt nematodes (Tylenchorhynchus spp.) are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated crops. These nematodes, with phyto-sanitary potential, are frequently ignored or misdiagnosed as pests, and this may pose a threat to food security. The accuracy of its identification based on a morphological approach has been challenged recently, due to the overlapping of the morphological and morphometric characters of the species. Consequently, the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops (wheat, maize and rice) in the savannahs of northern Nigeria, using integrative taxonomy and molecular approaches. The molecular and morphological studies identified and confirmed the presence of T. annulatus as the occurring specie in the savannahs of northern Nigeria. The phylogenetic analysis was carried out using the internal transcribed spacer (ITS) and 28S genes of ribosomal DNA further confirmed the presence of T. annulatus. The first molecular characterization and sequences of the ITS and 28S rDNA gene for T. annulatus from Nigeria were provided by this research. Also, according to our literature search, this is the first report on T. annulatus in wheat, maize and rice in the savannahs of northern Nigeria. Further study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies. 相似文献
5.
The present study was carried out using juveniles of silver pomfret, Pampus argenteus, produced in the hatchery by stripping eggs from spawners collected from the wild during 2004 and 2005 culture period. The experiments carried out during 2004 investigated the efficiency of using salmon feed mixed with cyclopeeze, salmon feed mixed with shrimp meat, salmon feed alone, and shrimp meat alone in the diet using 1 m3 capacity round fiberglass tanks in three replicates for each treatment with flow‐through seawater. The results showed that inclusion of shrimp meat in the diet either with salmon feed or alone give significantly high (P < 0.01) weight gain of up to 1.10 ± 0.06 g/fish/d and significantly lower (P < 0.001) feed conversion ratio compared to that of feeds without shrimp meat. The experiments carried out during 2005 investigated the use of commercially available feeds with feed additives. The results showed that feeding with “Gemma” feed with 54.0% crude protein and 19.0% crude fat or salmon feed with 41.4% crude protein and 23.9% crude fat give significantly high (P < 0.01) growth rates compared to that of pompano feed with 43.0% crude protein and 6.0% crude fat. The results of these investigations although show high growth rate of the fish compared to that of previous observations; there is yet an urgent need to formulate a suitable feed for this fish species for commercial ventures. 相似文献
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7.
Anna A. Filatenko Karl Hammer Sulaiman Al Khanjari Andreas Buerkert 《Genetic Resources and Crop Evolution》2010,57(8):1135-1139
Due to its geographic position on the northeastern tip of the Arabian Peninsula and its sea trade relationships with Asia,
East Africa and the Middle East, Oman has for millennia been at the cross-roads of inter-regional exchange of cultivated plants.
This is reflected in recent findings of new cultivars of banana (Musa spp.) and wheat (Triticum spp.) in remote oases of the Hajar Mountains in northern Oman. Material collected in 2003 and 2004 contained six new botanical
varieties of wheat which are described here. One of them belongs to the tetraploid T. aethiopicum, the others are hexaploid. 相似文献
8.
【目的】研究红宝石无核×红旗特早葡萄杂交后代核性状遗传规律。【方法】以红宝石无核×红旗特早杂交群体135个F1代植株为试材,鉴定核状态,测定果实单粒重、单核重、单粒核数量、单核纵横径积等核相关指标,分析群体核性状遗传倾向。【结果】红宝石无核×红旗特早杂交群体胚败育率为59.26%;果实单粒重表现为连续分布,遗传力低,呈现变小趋势;果实单核重在有核和无核单株群体之间存在两极分化,但没有明显界限,遗传力低,呈现变小趋势;果实单粒核数量遗传力低,呈现变少趋势,整个组合91.85%的单株具有败育残核;单核纵横径积在有核和无核单株群体之间存在两极分化,且有明显界限,遗传力低,呈现变小趋势,但在有核单株中表现增大趋势。【结论】红宝石无核与具有无核倾向的有核品种红旗特早杂交,可以获得较高比例的无核后代,群体在果粒质量、核质量、数量及纵横径积等指标上均表现为退化趋势,但在无核单株与有核单株群体之间存在一定差异,果粒质量、核质量、数量及纵横径积与核性状正相关联。 相似文献
9.
V Vescoli L Degiorgi W Henderson G Gruner KP Starkey LK Montgomery 《Science (New York, N.Y.)》1998,281(5380):1181-1184
Optical experiments were conducted on a series of organic linear chain conductors with different values of the interchain single-electron transfer integral tb, which quantifies the degree of anisotropy. Electron-electron interactions together with Umklapp scattering resulted in a correlation gap and an insulating state for small tb. An insulator-to-metal transition was observed when tb exceeded a critical value, on the order of the correlation gap Egap. The absence of a plasma edge on the insulator side of the transition for polarization perpendicular to the chains suggests that the electrons are confined to the chains. The optical features of the metallic state, when contrasted with the magnetic properties, are suggestive of spin-charge separation. 相似文献
10.
Wasiu A. Jimoh Mohd S. Kamarudin Muhammad A. Sulaiman Akeem B. Dauda 《Aquaculture Research》2019,50(11):3401-3411
The performance of selected leaf meals of high dietary fibre in the feed of a tropical commercial carp, hybrid lemon fin barb (Barbonymus gonionotus ♀ × Hypsibarbus wetmorei ♂) was evaluated in a 56‐day feeding trial. The tropical carp juveniles (9.43 ± 0.05 g) were randomly stocked in 60‐L aquaria at 15 fish per aquarium. Five isonitrogenous and isocaloric diets (30% crude protein, 17 kJ/g gross energy) containing no leaf meal and 10% napier grass (Pennisetum purpureum), alfalfa (Medicago sativa), water spinach (Ipomea aquatica) and Gliricidia sepium leaf meals were formulated and tested. Three aquaria were randomly assigned to each experimental diet. The results showed that fish‐fed diets containing leaf meals showed superior growth performance and body composition which was better than those fed control diet. The leaf meal‐treated groups also had higher values of protein, lipid, energy retention and production of digestive enzymes amylase. Conclusively, the results indicated that leaf meal fibre provided better performance showing the inherent prebiotic effect of the utilization of these leaf meal in hybrid lemon fin barb . 相似文献