不同增氧滴灌方式对蔬菜生长生理指标的影响   总被引：1，自引：0，他引：1  

臧明  雷宏军  BHATTARAI Surya  BALSYS Ron  徐建新 《排灌机械工程学报》2020,38(3):310-317

为了研究不同增氧方式对盆栽小白菜生长生理指标的影响,以小白菜为供试作物,采用盆栽地下滴灌的方式,以普通地下滴灌作为对照(CK),设置循环曝气(MAI)、双氧水(H₂O₂)、纯氧扩散器曝气(OC)及射流振荡器曝气(FO)4个增氧灌溉处理.结果表明,增氧地下滴灌显著提高了土壤呼吸速率,处理MAI,OC和FO较对照处理分别增大了65.87%,66.79%和111.62%.增氧地下滴灌促进了小白菜的根系生长、光合作用、蒸腾速率和气孔导度,进而提高了小白菜的物质量积累和产量.与对照相比,处理MAI的地下部鲜质量增大了42.03%,地下部干质量增大了79.85%;处理MAI,H₂O₂,OC和FO的光合速率分别增大了868.62%,794.14%,778.67%和650.19%;处理MAI,H₂O₂和OC的气孔导度较CK增大了157.14%,128.57%和85.71%,蒸腾速率增大了55.61%,32.38%和19.58%;处理MAI和H₂O₂的产量分别增大了56.36%和38.72%.综上,增氧地下滴灌可增强小白菜根区的土壤呼吸作用,改善光合作用、蒸腾速率和气孔导度,提高了产量及水分利用效率.其中,循环曝气处理的改善效果最为显著.  相似文献   

Effect of dietary Curcuma longa on enzymatic and immunological profiles of rohu,Labeo rohita (Ham.), infected with Aeromonas hydrophila     

Swagatika Sahu  Basanta Kumar Das  Bibhudendu Kumar Mishra  Jyotirmayee Pradhan  Surya Kanta Samal  Niranjan Sarangi 《Aquaculture Research》2008,39(16):1720-1730

The aim of this study was to evaluate the dietary dosages of turmeric that enhance immune response and disease resistance against the opportunistic pathogens Aeromonas hydrophila in Labeo rohita fingerlings. Hence, four different dosages of turmeric at 0.1, 0.5, 1.0 and 5.0 g kg^?1 feed were given to the fingerlings of L. rohita for 60 days at 4% body weight. At every 20‐day interval, different biochemical, haematological, enzymatic and immunological parameters of fish were evaluated. After 60 days, fish were challenged with A. hydrophila. The mortality (%) was recorded on the tenth day post challenge. Most of the immune parameters including lysozyme activity, superoxide anion production and serum bactericidal activity were significantly (P<0.05) higher on 60 days of feeding of 1.0 g of turmeric per kg of feed. Challenge study indicated 100% and 89% survivability in the group of fish fed with 5.0 and 1.0 g of turmeric per kg of feed respectively. Feeding of turmeric might have maintained long‐term protection in fish by elevating the nonspecific immune system such as Nitroblue tetrazolium (NBT), lysozyme and serum bactericidal activity. The result showed that turmeric at a dose of 1.0 g kg^?1 feed for 60 days provided the greatest protection to pathogen challenge.  相似文献   

Clinical trial of parvaquone for the treatment of East Coast fever in Kenya   总被引：1，自引：0，他引：1  

S Chema  S Waghela  A D James  T T Dolan  A S Young  W N Masiga  A D Irvin  G H Mulela  L S Wekesa 《The Veterinary record》1986,118(21):588-589

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Animal brucellosis in Kenya: a review.     

S Waghela 《Bulletin of animal health and production in Africa》1976,24(1):53-59

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A serological survey of the prevalence of brucellosis in pigs in Kenya.     

S Waghela  J M Gathuma 《Bulletin of animal health and production in Africa》1976,24(3):251-253

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Variation in diosgenin levels among 10 accessions of fenugreek seeds produced in western Canada   总被引：1，自引：0，他引：1  

Taylor WG  Zulyniak HJ  Richards KW  Acharya SN  Bittman S  Elder JL 《Journal of agricultural and food chemistry》2002,50(21):5994-5997

A collection of 10 accessions of fenugreek (Trigonella foenum-graecum L.), an annual legume, was grown during two summers at three plot locations in western Canada to assess whether genetic (accession) and environmental factors (site and year of production) influenced levels of diosgenin, a steroidal sapogenin. The 60 harvested seed samples, each analyzed by single determinations on three subsamples of defatted and dried seed material, were hydrolyzed by a microscale procedure in water containing 2-propanol (70%) and sulfuric acid (1 M). The extracts were analyzed by capillary gas chromatography with 6-methyldiosgenin as internal standard. Diosgenin levels from mature seeds ranged from 0.28 to 0.92% (28-92 microg/10 mg). Analysis of variance on combined diosgenin levels from the three sites and two years revealed that accession, accession x year, and site x year effects were significant for diosgenin content, whereas site, year, and site x accession effects were not. Four accessions, CN 19062, CN 19067, CN 19070, and CN 19071, were identified with high levels of diosgenin on the basis of the 2-year data set. In these accessions, mean levels of diosgenin plus yamogenin from seven site years were estimated at 0.70, 0.98, 0.84, and 0.87%, respectively.  相似文献   

Assessment of genetic relationships between <Emphasis Type="Italic">Rhus</Emphasis> L. species using RAPD markers     

Surya Prakash  Johannes Van Staden 《Genetic Resources and Crop Evolution》2007,54(1):7-11

Genetic diversity of seven Rhus L. species was assessed using random amplified polymorphic DNA (RAPDs) markers. Initially, 90 primers were screened, of which 25 produced reproducible amplification products. These primers generated a total of 296 bands, with an average of 11.8 bands per primer. Out of 296 bands scored, 236 (80%) were polymorphic and 62 (20%) were monomorphic. Primers OPC-05 and OPD-05 generated 100% polymorphic bands. The resolving power of primers ranged from 9.4 to 26.8. Similarity matrix values ranged from 0.45 to 0.63. The dendrogram generated using Unweighted Pair Group Method using Arithmetic Averages (UPGMA) grouped all the species of Rhus in one major group with two sister groups, whilst R. pyroides Burch. and R. dentata Thunb. were outliers. R. gerrardii (Harv. ex Engl.) Diels, R. glauca Thunb. and R. pentheri Zahlbr. constituted one sister group, while R. natalensis Bernh. ex C. Krauss and R. gueinzii Sond. were included in the other. The degree of genetic diversity observed between seven species of Rhus with RAPD markers suggest that this approach could be used for studying the phylogeny of the genus.  相似文献   

Serological response of cattle, sheep and goats in Kenya vaccinated with killed Brucella melitensis strain H38 adjuvant vaccine     

S Waghela 《The Veterinary record》1983,112(20):476-479

The antibody responses of cattle, sheep and goats to the Brucella melitensis H38 adjuvant vaccine were monitored by serum (tube) agglutination, complement fixation and Rose Bengal plate tests. High and persisting antibody titres were induced by a single dose of this vaccine in all three species. It would be difficult to classify reactors by commonly used diagnostic tests in a control or an eradication programme using the H38 adjuvant vaccine.  相似文献   

Phage display selection and characterization of single-chain recombinant antibodies against Eimeria tenella sporozoites     

Abi-Ghanem D  Waghela SD  Caldwell DJ  Danforth HD  Berghman LR 《Veterinary immunology and immunopathology》2008,121(1-2):58-67

A single-chain antibody library against Eimeria tenella sporozoites was constructed by phage display. Antibody-displaying phage was selected in five panning rounds against cryopreserved E. tenella sporozoites. A 1000-fold increase in phage output and a 3000-fold enrichment were obtained after three rounds of panning, as the binding clones became the dominant population in the library. Ten clones were randomly selected from the last selection round, and their nucleotide sequences were aligned and compared to chicken germ-line sequences. Analysis of the light chain variable regions revealed possible donor pseudogenes which act as donors in gene conversion events, and contribute to the diversification of the V(L) immune repertoire. Possible somatic hypermutation events, a consequence of affinity maturation, were also identified. Soluble antibody was produced in a non-suppressor E. coli strain, purified by nickel affinity chromatography, and characterized by immunoblotting. In an immunofluorescence assay, this recombinant antibody showed specific binding to E. tenella sporozoites.  相似文献   

Seroprevalence estimation and management factors associated with high herd seropositivity for <Emphasis Type="Italic">Babesia bovis</Emphasis> in commercial dairy farms of Puerto Rico     

J. H. Urdaz-Rodríguez  G. T. Fosgate  S. D. Waghela  A. R. Alleman  D. O. Rae  G. A. Donovan  P. Melendez 《Tropical animal health and production》2009,41(7):1465-1473

A cross-sectional study was conducted to determine individual cow seroprevalence of Babesia bovis in adult lactating dairy cattle of Puerto Rico (PR), to assess the associations of farm management factors on herd seroprevalence, and to document the species of ticks infesting cattle within these farms. Antibody activity against B. bovis was determined using an indirect fluorescent antibody test (IFAT). Serum samples were obtained from 2,414 adult lactating dairy cattle from 76 randomly selected commercial dairy farms. Herd seroprevalence ranged from 0 to 51% with an overall individual cow seroprevalence for B. bovis of 26%. Ticks were collected from animals on 7 (9%) of the 76 participating commercial dairy farms. All collected ticks (n = 87) were Rhipicephalus (Boophilus) microplus. Factors associated with high herd seropositivity were dairy farms with calf but not heifer raising facilities (OR = 16, 95% CI = 3.0-86), having more than 4 neighbors with cattle (OR = 17, 95% CI = 1.6-178), same producer owning more than one farm (OR = 7.2, 95% CI = 1.6-32), and use of government services to apply amitraz on cattle (OR = 5.5, 95% CI = 1.5-20).  相似文献