排序方式： 共有4条查询结果，搜索用时 15 毫秒
1
1.
Gurpreet K. Aulakh Sushmita Maltare Le Nguyen Phuong Khanh Baljit Singh 《Canadian journal of veterinary research》2021,85(3):161
The cell surface protein CD34 is expressed in various human tissues and cells, including hematopoietic stem cells, vascular endothelial cells, mucosal dendritic cells, mast cells, eosinophils, microglia, fibrocytes, muscle satellite cells, and platelets. There is a lack of data on the expression of CD34 in canine and porcine tissues. Therefore, we designed a series of immunoblotting, immunohistochemistry, and immunofluorescence experiments to observe CD34 expression in murine, canine, and porcine lungs. We used a rabbit antibody (clone EP373Y) to target the conserved human CD34 C-terminal region and validated its immunoreactivity against mouse lung homogenates. The data showed diffuse bronchiolar and alveolar epithelial localization of CD34 protein in normal murine, canine, and porcine lungs. At 9 or 24 h after bacterial endotoxin exposure, murine CD34 protein shifted to specific bronchoalveolar cells with a punctate pattern, as quantified by CD34 fluorescence. Specific porcine bronchoalveolar cells and leukocytes had significant CD34-positive immunostaining after H3N1 influenza infection. Thus, our study provides fundamental data on the expression of CD34 in lungs and validates an antibody for use in further experiments in these animal species. 相似文献
2.
Enzymes provide enormous rate enhancements, unmatched by any other type of catalyst. The stabilization of high-energy states along the reaction coordinate is the crux of the catalytic power of enzymes. We report the atomic-resolution structure of a high-energy reaction intermediate stabilized in the active site of an enzyme. Crystallization of phosphorylated beta-phosphoglucomutase in the presence of the Mg(II) cofactor and either of the substrates glucose 1-phosphate or glucose 6-phosphate produced crystals of the enzyme-Mg(II)-glucose 1,6-(bis)phosphate complex, which diffracted x-rays to 1.2 and 1.4 angstroms, respectively. The structure reveals a stabilized pentacovalent phosphorane formed in the phosphoryl transfer from the C(1)O of glucose 1,6-(bis)phosphate to the nucleophilic Asp8 carboxylate. 相似文献
3.
modENCODE Consortium Roy S Ernst J Kharchenko PV Kheradpour P Negre N Eaton ML Landolin JM Bristow CA Ma L Lin MF Washietl S Arshinoff BI Ay F Meyer PE Robine N Washington NL Di Stefano L Berezikov E Brown CD Candeias R Carlson JW Carr A Jungreis I Marbach D Sealfon R Tolstorukov MY Will S Alekseyenko AA Artieri C Booth BW Brooks AN Dai Q Davis CA Duff MO Feng X Gorchakov AA Gu T Henikoff JG Kapranov P Li R MacAlpine HK Malone J Minoda A Nordman J Okamura K Perry M Powell SK Riddle NC Sakai A 《Science (New York, N.Y.)》2010,330(6012):1787-1797
4.
Rhind N Chen Z Yassour M Thompson DA Haas BJ Habib N Wapinski I Roy S Lin MF Heiman DI Young SK Furuya K Guo Y Pidoux A Chen HM Robbertse B Goldberg JM Aoki K Bayne EH Berlin AM Desjardins CA Dobbs E Dukaj L Fan L FitzGerald MG French C Gujja S Hansen K Keifenheim D Levin JZ Mosher RA Müller CA Pfiffner J Priest M Russ C Smialowska A Swoboda P Sykes SM Vaughn M Vengrova S Yoder R Zeng Q Allshire R Baulcombe D Birren BW Brown W Ekwall K Kellis M Leatherwood J Levin H Margalit H Martienssen R 《Science (New York, N.Y.)》2011,332(6032):930-936
1