One-step RT-PCR for the Detection of Infectious Bursal Disease Virus in Clinical Samples     

Kataria RS  Tiwari AK  Nanthakumar T  Goswami PP 《Veterinary research communications》2001,25(5):429-436

A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA.  相似文献   

Evaluation of pulp and paper making characteristics of certain fast growing plants     

C. N. Saikia  T. Goswami  F. Ali 《Wood Science and Technology》1997,31(6):467-475

Summary Four species of fast growing annual plant viz.Hibiscus sabdariffa, Crotalaria juncea, Tephrosia candida andHibiscus cannabinus and a variety of reed, Neyraudia reynaudiana were evaluated in the laboratory for their pulp and paper making properties. Data on proximate chemical analysis of raw materials, unbleached and bleached pulp properties, morphological properties of fibres and physical strength properties of paper sheets were evaluated. The unbleached pulp yields were 44.90–53.20% with all the five plant materials, while bleached pulp yields varied from 39.80–50.60%. The average fibre lengths and diameters of the unbleached pulp obtained from the five plant species were within the range of 0.75 mm–2.15 mm and 20–22 pun respectively. Paper hand sheets, made with bleached pulps (45° SR) obtained from the five plant species, gave burst indices 2.75–3.92 kPa m²/g, tear indices 3.73–9.61 m N m²/g and tensile indices 37.27–47.81 N mg^–1, indicating adequate strength properties for writing and printing type of paper.The authors thank Director, Regional Research Laboratory (CSIR), Jorhat, India, for his kind permission to publish this paper. The authors also wish to thank B. N. Tosh, JPF for his assistance.  相似文献   

Performance of F1 progenies developed through crosses between Brassica carinata A. Braun A(♀) and Brassica rapa L (♂)     

Goswami  Barna  Momtaz  Nasima  Hoque  M. I.  Sarker  R. H. 《Genetic Resources and Crop Evolution》2022,69(8):2745-2753

Genetic Resources and Crop Evolution - The present investigation was aimed to study the performance of F1 progenies of crosses between Brassica carinata A. Braun and Brassica rapa L. considering...  相似文献   

Effect of body weight at birth of the Murrah buffaloes on their milk yield and period of different lactations     

S B Goswami  J D Joshi 《The Indian veterinary journal》1970,47(3):228-236

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The effect of administration of thyroxine and PMS hormones on blood corpuscular counts of buffalo bulls (Bubalus bubalis)     

S B Goswami 《The Indian veterinary journal》1965,42(9):670-674

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Pathogenicity and In Planta Mycotoxin Accumulation Among Members of the Fusarium graminearum Species Complex on Wheat and Rice     

Goswami RS  Kistler HC 《Phytopathology》2005,95(12):1397-1404

ABSTRACT Fusarium head blight (FHB), or scab, is a destructive disease of small grains caused by members of the Fusarium graminearum species complex, comprised of at least nine distinct, cryptic species. Members of this complex are known to produce mycotoxins including the trichothecenes deoxynivalenol (DON) along with its acetylated derivatives and nivalenol (NIV). In this study, 31 strains, belonging to eight species of this complex and originating from diverse hosts or substrates, were tested for differences in aggressiveness and mycotoxin production. Large variation among strains, both in terms of their aggressiveness and the ability to produce trichothecenes on a susceptible cultivar of wheat was found; variation appears to be a strain-specific rather than species-specific characteristic. While pathogenicity was not influenced by the type of mycotoxin produced, a significant correlation was observed between the amount of the dominant trichothecene (DON and its acetylated forms or NIV) produced by each strain and its level of aggressiveness on wheat. Some isolates also were tested for their ability to infect rice cv. M201, commonly grown in the United States. While tested strains were capable of infecting rice under greenhouse conditions and causing significant amount of disease, no trichothecenes could be detected from the infected rice florets.  相似文献   

Relative potencies of natural estrogens on vitellogenin and choriogenin levels in the Indian freshwater spotted snakehead, <Emphasis Type="Italic">Channa punctata</Emphasis>: in vivo and in vitro studies     

K. V. Rani  N. Sehgal  S. V. Goswami  Om Prakash 《Fish physiology and biochemistry》2010,36(3):587-595

The relative efficacies of three natural estrogens viz., estrone (E₁), estradiol-17β (E₂) and estriol (E₃) to induce synthesis of vitellogenin (Vg) and choriogenin (Chg) were assessed in primary hepatocyte cultures of the Indian freshwater spotted snakehead, Channa punctata. Hepatocytes were isolated from the spotted snakehead liver by a non-enzymatic protocol. Optimum culture conditions were standardized for ensuring their viability and functioning. Isolated hepatocytes were cultured for 48 h for monolayer formation and then exposed to various concentrations (0.001–10 μM) of the three estrogens. Competitive homologous ELISAs, developed and validated for spotted snakehead Vg and Chg were employed to determine the amounts of these two proteins secreted into the culture medium after 48 h of incubation. The results reveal that although all the three estrogens were effective in inducing the production of Vg and Chg in a dose-dependent manner, there were differences in their relative potencies. Of three estrogens, E₁ was the least potent and could induce synthesis of Vg and Chg only at a minimum concentration of 0.5 μM; whereas significant levels of both the proteins were quantified in culture medium by exposing the hepatocytes to E₂ or E₃ even at a concentration of 0.001 μM. All three estrogens were effective in inducing synthesis of Vg and Chg in vivo also. These results suggest the possibility of employing the above in vitro experimental design to monitor the presence of estrogens/estrogen-like chemicals in natural waters, which could interfere with the estrogen receptor system of fish. This study further points to the possibility of using Chg, in addition to Vg, as a parameter for screening various chemicals for their estrogenic activity.  相似文献   

Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.)     

Yadav K  Lakra WS  Sharma J  Goswami M  Singh A 《Fish physiology and biochemistry》2012,38(4):1035-1045

Tor tor is an important game and food fish of India with a distribution throughout Asia from the trans-Himalayan region to the Mekong River basin to Malaysia, Pakistan, Bangladesh and Indonesia. A new cell line named TTCF was developed from the caudal fin of T. tor for the first time. The cell line was optimally maintained at 28°C in Leibovitz-15 (L-15) medium supplemented with 20% fetal bovine serum (FBS). The propagation of TTCF cells showed a high plating efficiency of 63.00%. The cytogenetic analysis revealed a diploid count of 100 chromosomes at passage 15, 30, 45 and 60 passages. The viability of the TTCF cell line was found to be 72% after 6 months of cryopreservation in liquid nitrogen (-196°C). The origin of the cell lines was confirmed by the amplification of 578- and 655-bp sequences of 16S rRNA and cytochrome oxidase subunit I (COI) genes of mitochondrial DNA (mtDNA) respectively. TTCF cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids. Further, immunocytochemistry studies confirm its fibroblastic morphology of cells. Genotoxicity assessment of H?O? in TTCF cell line revealed the utility of TTCF cell line as in vitro model for aquatic toxicological studies.  相似文献   

Development of an ES-like cell culture system (RESC) from rohu, Labeo rohita (Ham.)     

M. Goswami  W. S. Lakra  Kamalendra Yadav  J. K. Jena 《Fish physiology and biochemistry》2012,38(6):1775-1783

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Interactive Effects of Nitrogen Source and Salinity on Growth Indices and Ion Content of Indian Mustard     

N. S. Nathawat  M. S. Kuhad  C. L. Goswami  A. L. Patel  Rakesh Kumar 《Journal of plant nutrition》2013,36(4):569-598

ABSTRACT

The interactions between salinity and different nitrogen (N) sources nitrate (NO₃ ^?), ammonium (NH₄ ⁺), and NO₃ ^? + NH₄ ⁺ were investigated on Indian mustard (Brassica juncea cv. RH30). Treatments were added to observe the combined effect of two salinity levels (8 and 12 ds m^{? 1}) and three nitrogen sources (NO₃ ^?, NH₄ ⁺, and NO₃ ^? + NH₄ ⁺) on different growth parameters and mineral composition in different plant parts, i.e., leaves, stem, and root. Salinity has been known to affect the uptake and assimilation of various essential nutrients required for normal growth and development. Different growth parameters, i.e., leaf area, dry weight of different plant parts, absolute growth rate (AGR), relative growth rate (RGR), and net assimilation rate (NAR) declined markedly by salinity at pre-flowering and flowering stages. All growth indices were less sensitive to salinity (12 d s m^{? 1}) with the nitrate form of nitrogen. It is pertinent mention that a high dose (120 kg ha^{? 1}) of nitrogen in ammonium form NH₄ ⁺, acted synergistically with salinity in inhibiting growth. Plants fed with combined nitrogen (NO₃ ^? + NH₄ ⁺) had an edge over individual forms in ameliorating the adverse effects of salinity on growth and yield. Under salt stress, different nutrient elements such as N, phosphorus (P), potassium (K⁺), and magnesium (Mg^{2 +}) were decreased in different plant parts (leaves, stem, and root). The maximum and minimum reduction was observed with ammoniacal and combined form of nitrogen, respectively, while the reverse was true of calcium (Ca^{2 +}), sodium (Na⁺), chloride (Cl^?), and sulfate (SO₄ ^2?) at harvest. Nitrogen application (120 Kg ha^{? 1}) in combined form had been found to maintain highest concentrations of N, P, Mg^{2 +}, and Ca^{2 +} along with reduced concentrations of Na⁺, Cl^?, and SO₄ ^{2 ?}. However, reverse was true with ammoniacal form of nitrogen.  相似文献