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1.
苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析 总被引：1，自引：0，他引：1
以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。 相似文献
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Chris Garforth Kevin McKemey Tahir Rehman Richard Tranter Richard Cooke Julian Park Peter Dorward Chris Yates 《Livestock Science》2006,103(1-2):158-168
Unidentified heats contribute to declining fertility rates in English dairy herds. Several techniques have been advocated to improve heat detection rates. Despite demonstrable technical efficacy and cost-effectiveness, uptake is low. A study in South West England used the Theory of Reasoned Action (TORA) to explore dairy farmers' attitudes and beliefs towards heat detection techniques. Few farmers were convinced that following prescribed observation times, milk progesterone testing and using pedometers would fit their system or improve on their current heat detection practices. Perceived difficulty of using a technique was not a constraint on adoption. Without promotion that addresses identified barriers and drivers to adoption, little change in current practice can be expected. 相似文献
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Remobilization of Nitrogen and Carbohydrate from Stems of Bread Wheat in Response to Heat Stress during Grain Filling 总被引：3，自引：0，他引：3
When wheat (Triticum aestivum L.) is grown under heat-stress conditions during grain filling, preanthesis stored total non-structural carbohydrates (TNC) and nitrogen (N) could serve as alternative source of assimilates. This study was performed to evaluate wheat genotypes for their ability to accumulate and remobilize TNC and N stored in their stem to support grain filling under heat stress. Eighteen genotypes were used for N remobilization study while nine of them were used for TNC remobilization study. They were grown in pots and placed in a vinyl house with the maximum temperature kept below 30 °C. Five days after anthesis (5DAA), half of the pots were taken to phytotrons where temperature was gradually increased and the maximum was set at 38 °C. Grain yield and grain weight decreased by about 35 % under heat stress. Significant differences were found among genotypes in percentage reduction in grain yield, grain weight, grain filling duration and harvest index because of heat stress. The N and TNC concentrations of the stem at 5DAA were significantly different among genotypes. Heat stress significantly reduced the N remobilization efficiency of most of genotypes. However, heat stress significantly increased TNC remobilization efficiency and significant variation were observed among genotypes. N remobilization efficiency across treatments significantly correlated with grain yield, grain weight, harvest index and grain filling duration. TNC at 5DAA negatively correlated with N at 5DAA and harvest index, but the TNC remobilization efficiency under heat stress positively correlated with mainstem grain yield, grain weight and harvest index. The rate of chlorophyll loss from flag leaf positively correlated with N and TNC remobilization efficiencies under heat stress suggesting a link between leaf senescence and remobilization efficiency. The results indicate that heat stress negatively affected grain yield, its components and N remobilization while it increased TNC remobilization because of the increasing demand for resources. 相似文献
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Studies were conducted on denitrification in the plough layer of an irrigated sandy-clay loam under a wheat-maize cropping
system receiving different fertilizer treatments. The treatments were: N-100 (urea-N at 100kgha–1year–1), N-200 (urea-N at 200kgha–1year–1), FYM-16 (farmyard manure at 16 tonnes ha–1year–1), FYM-32 (farmyard manure at 32 tonnesha–1year–1) and the control (unfertilized). Averaged across sampling dates during the wheat season, the denitrification rate as measured
by the C2H2-inhibition/soil-core incubation method was highest in N-200 (83gNha–1day–1), followed by FYM-32 (60gNha–1day–1, N-100 (51gNha–1day–1), FYM-16 (47gNha–1day–1) and the control (33gNha–1 day–1). During the maize growing season, average denitrification rate was highest in FYM-32 (525gNha–1day–1), followed by FYM-16 (408gNha–1day–1), N-200 (372gNha–1day–1, N-100 (262gNha–1day–1) and the control (203gNha–1day–1). Denitrification loss integrated over the whole vegetation period was at a maximum under FYM-32 (13.9kgNha–1), followed by N-200 (11.8kgNha–1), FYM-16 (10.6kgNha–1) and N-100 (8.0kgNha–1), whereas the minimum was observed for the control (5.8kgNha–1). Under both crops, denitrification was significantly correlated with water-filled pore space and soil NO3
–-N. The best multiple regression models accounted for 52% and 70% of the variability in denitrification under wheat and maize,
respectively. Results indicated that denitrification is not an important N loss mechanism in this well-drained, irrigated
sandy-clay loam under a wheat-maize cropping system receiving fertilizer inputs in the range of 100–200kgNha–1year–1.
Received: 14 January 1997 相似文献
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Purpose
Crop growth on sandy soils can be increased by claying. In modified sandy soils, the added clay is in the form of peds ranging in size from millimetres to centimetres creating a highly non-uniform matrix where ped size could influence nutrient availability and organic C binding. The aim of the study was to determine the effect of clay addition rate and ped size in residue amended sandy soil on soil respiration, nutrient availability and organic C retention.Materials and methods
In this study, clay peds of 1, 2 or 3 mm size derived from a clay-rich Vertosol (73 % clay) were added to a sandy soil (3 % clay) at clay addition rates of 10 and 20 % w/w. After the addition of ground faba bean residue (C/N 37) at 10 g kg?1, the soils were incubated for 45 days at 80 % of water-holding capacity.Results and discussion
Clay addition had no consistent effect on cumulative respiration, but reduced NH4 + availability with a greater reduction at 20 % compared to 10 % clay and with 1 and 2 mm compared to 3 mm peds. Sandy soil with clay peds had a greater maximum NH4 + and P sorption capacity than sandy soil alone, and sorption capacity was higher at 20 % compared to 10 % clay addition and greater with 1 mm compared to 3 mm peds. Retrieval of clay peds at the end of the experiment showed ped breakdown during the experiment but also the formation of larger peds. Compared to the <53 μm fraction added at the start of the experiment, the total organic carbon (TOC) content of the <53 μm fraction was increased up to nearly two fold, particularly in the smaller peds (1 and 2 mm).Conclusions
When sandy soils are amended with clay, N availability and organic C binding depend on both clay addition rate and ped size.9.
The interactions between the adsorption characteristics of 27 experimental soils and the sorption of Cd and Ni from the municipal wastewaters were investigated in this study. The removal of these elements from soil solution was followed for 50 days. All the adsorption characteristics, except cation exchange capacity and organic matter, were significantly correlated to the sorption of Cd after one day shaking. After 7 days of shaking, none of the soil adsorption characteristics except free CaCO3 was significantly correlated to Cd removal from wastewater. The soil saturated paste pH and suspension pH were strongly correlated to Cd sorption throughout this experiment. The behavior of Ni in soils was different from that of Cd. Surface area, total Fe, and total Al were significantly correlated to Ni sorption. The correlation between Ni removal and pH was the strongest than any other parameter studied. After 7 days shaking, clay content and total Ca were not significantly correlated to Ni sorption. The cation exchange capacity of the soils was not significantly correlated to Cd or Ni sorption in this experiment. It seems that in the experimental soils, concentration of Cd and Ni were probably not controlled by adsorption process. The precipitation process was probably playing a major role in the removal of these elements from the municipal wastewaters. As observed in this experiment, the cation exchange capacity of experimental soils was a poor parameter to define sorption capacity of these soils for Cd and Ni. The guidelines for determining the soil sludge load, which are mainly based on the cation exchange capacity of soils, should be revised. 相似文献
10.
In order to identify the factors influencing adoption of technologies promoted by government to small-scale dairy farmers in the highlands of central Mexico, a field survey was conducted. A total of 115 farmers were grouped through cluster analysis (CA) and divided into three wealth status categories (high, medium and low) using wealth ranking. Chi-square analysis was used to examine the association of wealth status with technology adoption. Four groups of farms were differentiated in terms of farms' dimensions, farmers' education, sources of incomes, wealth status, management of herd, monetary support by government and technological availability. Statistical differences (p < 0.05) were observed in the milk yield per herd per year among groups. Government organizations (GO) participated little in the promotion of the 17 technologies identified, six of which focused on crop or forage production and 11 of which were related to animal husbandry. Relatives and other farmers played an important role in knowledge diffusion and technology adoption. Although wealth status had a significant association (p < 0.05) with adoption, other factors including importance of the technology to farmers, usefulness and productive benefits of innovations together with farmers' knowledge of them, were important. It is concluded that the analysis of the information per group and wealth status was useful to identify suitable crop or forage related and animal husbandry technologies per group and wealth status of farmers. Therefore the characterizations of farmers could provide a useful starting point for the design and delivery of more appropriate and effective extension. 相似文献