Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene     

Naoto NISHIMURA  Hiroki UEHARU  Hiroto NISHIHARA  Shiori SHIBUYA  Saishu YOSHIDA  Masashi HIGUCHI  Naoko KANNO  Kotaro HORIGUCHI  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2016,62(1):93-102

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Purification and characterization of feline ghrelin and its possible role     

Ida T  Miyazato M  Naganobu K  Nakahara K  Sato M  Lin XZ  Kaiya H  Doi K  Noda S  Kubo A  Murakami N  Kangawa K 《Domestic animal endocrinology》2007,32(2):93-105

Ghrelin, a novel 28-amino acid peptide with an n-octanoyl modification at Ser3, has been isolated from rat and human stomach as an endogenous ligand for the growth hormone secretagogue receptor. Here, we purified feline ghrelin and examined its possible physiological role in cats. The major active form of feline ghrelin is a 28-amino acid peptide octanoylated (C8:0) at Ser3; except for one amino acid residue replacement, this structure is identical to those of rat and human ghrelins. However, much structural divergence in peptide length and fatty acid modification was observed in feline ghrelin: peptides consisting of 27 or 26 amino acids lacking Gln14 and/or Arg28 were found, and the third serine residue was modified by octanoic acid (C8:0), decanoic acid (10:0), or unsaturated fatty acids (C8:1, C10:1 and C10:2). In agreement with the structural divergence, two kinds of cDNA with different lengths were isolated. Administration of synthetic rat ghrelin increased plasma growth hormone levels in cats, with a potency similar to that in rat or human. Plasma levels of ghrelin in cats increased approximately 2.5-fold after fasting. The present study indicates the existence of structural divergence in feline ghrelin and suggests that, as in other animals, ghrelin may play important roles in GH release and feeding in cats.  相似文献   

Spatial association between northern fur seal (Callorhinus ursinus) and potential prey distribution during the wintering period in the northern Sea of Japan          下载免费PDF全文

Takanori Horimoto  Yoko Mitani  Yasunori Sakurai 《Fisheries Oceanography》2016,25(1):44-53

Elucidating the response of marine predators to oceanographic features helps in understanding their foraging strategies and the cause of their spatial overlap and interference with fishery activities. Northern fur seal is a highly pelagic species during the wintering period. In recent years, fur seals have consistently been found distributed near the coast of southwest Hokkaido, Japan. Because interference by fur seals with coastal fishery activities has become a serious concern, an understanding is sought as to why fur seals come ashore in this area. We conducted ship‐based observations and estimated fur seal density. To elucidate the spatial and seasonal association of fur seal distribution and ocean environments, we constructed statistical models to describe how potential prey distribution and oceanographic features influenced the fur seal spatial distribution during the wintering period. Fur seal distribution corresponded markedly to potential prey distribution, and they tended to aggregate along the narrow continental shelf that is the main geographic feature of this area, which is 2000 m deep and approximately 10 km from the coast. Walleye pollock [Gadus chαlcogrαmmα (Theragra chαlcogrαmmα)] and arabesque greenling (Pleurogrammus azonus), which are one of the main prey for fur seals, move to the shallow area to spawn on the continental shelf seasonally, so potential prey abundance could be higher in coastal areas than offshore. Moreover, the absence of a mesopelagic biotic community may be one of the main factors in the coastal distribution of the fur seal.  相似文献   

A new viral epizootic of Anguilla japonica Temminck and Schlegel     

T. SANO  N. OKAMOTO  T. NISHIMURA 《Journal of fish diseases》1981,4(2):127-139

Abstract. In 1969, a new kind of epizootic occurred among eels in Japan and virological investigation was initiated. A new virus designated eel virus european (EVE) was isolated and biological, cytological, serological tests and infectivity trials were carried out. In its CPE on RTG-2 cells and additionally in its biological properties and particle size, EVE was found to be similar to infectious pancreatic necrosis virus (IPNV). Serologically, EVE was similar to the ď Honnincthun, France, strain of IPNV. However, infectivity trials showed that EVE and IPNV differed; EVE killed Japanese eels Anguilla japonica but not rainbow trout fry Salmo gairdneri , while IPN virus killed rainbow trout fry but not eels. We consider EVE to be the primary agent causing the new epizootic and propose the name viral kidney disease for the resulting clinical condition.  相似文献   

Invasion and colonization of mature apple fruit by Erwinia amylovora tagged with bioluminescence genes     

Koji Azegami  Takanori Tsukamoto  Takayuki Matsuura  Tatsuji Ohara  Yasuhiro Inoue  Akifumi Mizuno  Kouji Yoshida  Hideo Bessho  Shigeru Kimura  Masao Goto 《Journal of General Plant Pathology》2005,71(1):98-98

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Proportions of melanocyte stimulating hormone-immunoreactive cells in the adenohypophysis of Silky fowl and hyperpigmentation-free cockerels     

Shotaro NISHIMURA  Hitomi SHIMODA  Ichiro OSHIMA  Yoshitaka ONO  Kaoru OKANO  Akira ISHIBASHI  Shoji TABATA  Hisao IWAMOTO 《Animal Science Journal》2005,76(6):575-579

Alpha‐melanocyte stimulating hormone (α‐MSH) is one of the main regulators for melanocytes, and the adenohypophysis is one of the major tissues for the synthesis of this hormone. The Silky fowl is a characteristic breed of chicken with hyperpigmentation throughout the body. The involvement of the adenohypophysis in the hyperpigmentation of this breed is not known. In the present study, the proportion of melanocyte stimulating hormone‐immunopositive cells (MSH cells) in the adenohypophysis was immunocytochemically compared between Silky, Red Cornish × New Hampshire (RN) crossbred and Japanese bantam cockerels at 15 weeks of age. After the body and gland were weighed, the adenohypophyseal cells were enzymatically dispersed and immunostained for α‐MSH, and the immunopositive MSH cells were counted. The weights of the body and adenohypophysis were heaviest in the RN crossbred, followed by the Silky and lightest in the bantam cockerels. In contrast, the ratio between adenohypophysis and bodyweight was much larger in the Silky and the bantam than in the RN crossbreed (P < 0.05). The population of MSH cells in the adenohypophysis was larger in the Silky (14.3%) than in the RN crossbreed (8.0%) and the bantam (8.1%) cockerels (P < 0.05). From these results, it was concluded that prepubertal Silky cockerel have numerous MSH cells in the adenohypophysis suggesting a relationship to hyperpigmentation.  相似文献   

Characterization of an endopolygalacturonase Gene cppg1 from Phytopathogenic Fungus Chondrostereum purpureum     

Mineo SENDA  Takanori NARITA  Shinji AKADA  Toshikatsu OKUNO  Kazuo MIYAIRI 《Journal of General Plant Pathology》2001,67(1):41-44

Chondrostereum purpureum, a phytopathogenic fungus, produces endopolygalacturonase (endoPG) which has been suggested to have a causal role in the silver-leaf symptom of apple trees. In this paper, we detected C. purpureurn-derived endoPG at the infection sites using ELISA with a polyclonal antibody against endoPG I. A gene encoding endoPG I and its homolog were also isolated from the C. purpureum genome. The endoPG I gene was designated as cppg1. The cppg1 gene is the first fungal endoPG gene reported in the Basidiomycetes. Received 31 May 2000/ Accepted in revised form 13 September 2000  相似文献   

Hepatocyte growth factor transduces different intracellular signals in aortic and umbilical venous endothelial cells   总被引：1，自引：0，他引：1  

Makondo K  Kimura K  Kitamura T  Yamaji D  Saito M 《The Japanese journal of veterinary research》2003,51(2):105-112

Endothelial cells are important for maintenance of vascular integrity by producing a variety of bioactive molecules such as nitric oxide (NO). Recent evidence has suggested that there are some differences in characteristics between endothelial cells from different origins. Here we examined responses of two typical endothelial cells to hepatocyte growth factor (HGF), which induces endothelium-dependent relaxation of microvessels. Stimulation of human umbilical vein endothelial cells (HUVEC) and bovine aortic endothelial cells (BAEC) with HGF increased endothelial NO synthase activity, accompanied with an increase of activity-related site-specific phosphorylation of protein kinase B/Akt. However, HGF stimulated phosphorylation of p38 mitogen-activated protein kinase (MAPK) only in HUVEC, but not in BAEC, while it induced phosphorylation of p44/p42 MAPK in both cells. These results suggest that HGF transduces different intracellular signals between aortic and umbilical venous endothelial cells, and that the differences might represent divergent endothelial responses to growth factors, especially those that activate receptor-tyrosine kinases.  相似文献   

Isolation of Salmonella from diarrheic feces of pigs     

Asai T  Otagiri Y  Osumi T  Namimatsu T  Hirai H  Sato S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):159-160

A survey of Salmonella was carried out in fecal samples of 887 pigs with diarrhea collected from 235 pig farms between April 1996 and March 2001. Salmonella was isolated from 84 feces (9.5%) of 887 pigs and from 45 (19.1%) of 235 farms. The higher prevalence was found in weaned pigs (12.4%) and fattening pigs (17.3%) than in sows (4.2%) and suckling pigs (4.5%). Isolation rates of S. Typhimurium were higher from weaned and fattening pigs than from the others. Therefore, risk of horizontal infection of S. Typhimurium will increase, if no adequate health managements are practiced when weaned and fattening pigs have diarrhea.  相似文献   

Isolation and serological survey of Salmonella in pigs in Japan     

Asai T  Fujii S  Osumi T  Otagiri Y  Namimatsu T  Sato S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1011-1015

A total of 267 fecal and serum samples collected from individual pigs reared on a Salmonella-positive farm were subjected to bacteriological and serological examinations of Salmonella. Salmonella was isolated from 47 pigs (17.6%) and prevalence of antibody to lipopolysaccharide (LPS) of S. Typhimurium, which was partly common to S. O4, 12: d: -, was observed in 90 pigs (33.7%). Salmonella was isolated from 26 (28.9%) of 90 antibody-positive pigs and 21 (11.9%) of 177 antibody-negative pigs. Twenty-one of 36 pigs (58.3%) positive for S. O4, 12: d: -, five of 10 pigs (50.0%) positive for S. Havana, and none for S. Anatum had antibodies. Thus, seropositive rates were higher than isolation-positive rates, and antibody prevalence was associated with serovars of the isolates. Then, we analyzed antibody prevalence among pigs on Japanese pig farms. The antibodies to LPS of S. Typhimurium were found in 195 of 1,498 pigs (13.0%) and in at least one serum sample on 35 of 52 farms (67.3%). Our results indicate that Salmonella does not seem to be so prevalent in pigs though it is widely prevalent among pig farms.  相似文献