Reproductive cycle and growth of the sand dollar Scaphechinus griseus inhabiting Japanese surf clam Pseudocardium sachalinense fishing grounds in Tomakomai,southwest Hokkaido,Japan     

Sakurai  Izumi  Abe  Honami  Ogata  Takashi 《Fisheries Science》2019,85(3):465-474

Fisheries Science - Bioturbation by the sand dollar Scaphechinus griseus is suspected to inhibit the recruitment of Pseudocardium sachalinense, a commercially important bivalve. This study aimed to...  相似文献   

Apocrine adenocarcinoma in a golden hamster.     

F Urayama  T Sato  H Shibuya  W Shirai  M Matsutani  R Yamazaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1249-1252

An apocrine adenocarcinoma was observed in the subcutis of the abdomen of golden hamster. Histologically, the tumor cells irregularly formed multiple layers of cysts and some detached cells were presented in the cystic space. PAS stain with alpha-amylase digestion revealed PAS-positive alpha-amylase-resistant granules in the cytoplasm. Immunohistochemically, cytokeratin was demonstrated in the tumor cells. By electron microscopy, the tumor cells had an oval nucleus with invagination, abundant cytoplasmic organelles and microvilli protruding into the intercellular spaces.  相似文献   

Reproductive biology of the female Japanese mantis shrimp Oratosquilla oratoria (Stomatopoda) in relation to changes in the seasonal pattern of larval occurrence in Tokyo Bay, Japan     

Keita  KODAMA  Takamichi  SHIMIZU  Takashi  YAMAKAWA  Ichiro  AOKI 《Fisheries Science》2004,70(5):734-745

ABSTRACT:   The reproductive traits and the monthly larval abundance of the mantis shrimp Oratosquilla oratoria were investigated in Tokyo Bay, Japan, in 2002. The goal of the study was to elucidate the cause of changes in the monthly pattern of larval abundance from the 1980s to the 1990s as these changes relate to variation in the stock size of the adult shrimp. Oogenesis was divided into 10 stages by histological observation. The developmental stage of oocytes in an individual's ovary was synchronous, suggesting that almost all the oocytes in an ovary are spawned at the same time. The size at first maturity was estimated to be 7 ≤ body length ( BL ) < 8 cm. Fecundity was expressed as a function of BL , ranging from 19 300 eggs for 8 cm BL to 92 100 eggs for 14 cm BL . Small female shrimps (<10 cm BL ) spawned around August. Most large female shrimps (≥10 cm BL ) spawned around May, and some large female shrimps also spawned until September. Although most large female shrimps spawned in spring, the larval abundance was low before July and high from August onwards. The results suggest that a substantial decrease in the stock size of large individuals causes the low larval abundance before July.  相似文献   

Fluid accumulation in mouse ligated intestine inoculated with the vascular permeability factor produced by Bacillus cereus.   总被引：1，自引：0，他引：1  

K Shinagawa  K Sato  H Konuma  N Matsusaka  S Sugii 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):167-171

Partially purified vascular permeability (VP) factor (VPF) of Bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (MIL) and rabbit (RIL), suggesting that the VP activity may correlate with fluid accumulation in ligated intestinal loops of these animals. Fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (IDU) of partially purified VPF, whereas it was found at 2 hr in all loops with 400-600 IDU of partially purified VPF. In rabbit intestinal loops with 120-190 IDU of partially purified VPF, fluid accumulation was observed at 6 hr. From these findings, VPF produced by B. cereus can be easily detected in both MIL and RIL. The intestinal tissue of mouse intestinal loops was histopathologically damaged at different concentrations of the VPF to induce fluid accumulation. With 50 IDU of partially purified VPF, severe edema was found in the laminia proprial layer and submucosa. With 600 IDU of partially purified VPF, on the other hand, severe necrosis in the surface epithelium of villus and laminia proprial layer, and hyperemia in the submucosa were observed, suggesting that partially purified VPF may be cytotoxic and/or intestinecrotic.  相似文献   

Acanthochondria priacanthi (Copepoda: Chondracanthidae) as a biological indicator for stock identification of sandfish Arctoscopus japonicus (Steindachner)     

Takashi  YANAGIMOTO  Kenji  KONISHI 《Fisheries Science》2004,70(2):336-338

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Expressed sequence tag of Japanese flounder Paralichthys olivaceus skin cells     

Ikuo  HIRONO  Ryosuke  YAZAWA  Takashi  AOKI 《Fisheries Science》2004,70(1):195-197

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Low incidence of Enterobacteriaceae in wild ducks (Aythya spp.) and antibiograms of the isolates.     

G Sato  M Asagi  H Yamamoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1979,41(2):181-183

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Purification and properties of pyrethroid carboxyesterase in rat liver microsome     

Takashi Suzuki  Junshi Miyamoto 《Pesticide biochemistry and physiology》1978,8(2):186-198

Pyrethroid carboxyesterase which hydrolyzes the esters of chrysanthemumic acid was purified from rat liver microsome by cholic acid solubilization, ammonium sulfate fractionation, heat treatment, and DEAE-Sephadex A-50 column chromatography. The 45-fold purified enzyme (38% yield) is likely to consist of single protein, as evidenced by polyacrylamide gel disc electrophoresis and Sephadex G-100 column chromatography, and had a molecular weight of approximately 74,000 and a K_m of 0.21 mM. It is susceptible to inhibition by organophosphates and carbamate insecticides and insensitive to pCMB, mercuric ion, and cupric ion. It is capable of hydrolyzing trans isomers of synthetic pyrethroids much more rapidly (five to ten times) than the cis counterparts. The purified pyrethroid carboxyesterase is apparently identical in nature with malathion carboxyesterase and with p-nitrophenyl acetate carboxyesterase.  相似文献   

Human interleukin-1β induces A375-S2 human melanoma apoptosis through caspase pathway     

WANG Che  WANG Min-wei  TASHIRO Shin-ichi  ONODERA Satoshi  IKEJIMA Takashi 《园艺学报》2004,20(7):1138-1143

AIM: To study the molecular biological mechanism and signal transduction pathway of interleukin-1β (IL-1β)-induced apoptosis in A375-S2 melanoma cells. METHODS: Photomicrocropy showed typical apoptotic changes. The cytotoxic effect of IL-1β in vitro and influences of caspases in this effect were measured by MTT assay. The cytotoxicity of cells was assessed by LDH-based assay. Degradation of DNA was detected by agarose gel electrophoresis. RESULTS: The inhibitory effect of IL-1β on A375-S2 cell growth was in a dose and time-dependent manner, and cell death rate reached more than 90% at 72 h after treatment with 10^-9mol/L IL-1β. The inhibitors of caspase-family, -1, -3, -8, -9, and -10, partially blocked cell death at early stage. LDH assay showed that major IL-1β-induced cell death was apoptosis, and in a dose and time-dependent manner. Typical apoptotic DNA ladder was observed in agarose gel electrophoresis. CONCLUSION: IL-1β induced apoptosis in melanoma A375-S2 cells by activating caspase pathway.  相似文献   

Evaluation of cross-protection with an attenuated isolate of Bean yellow mosaic virus by differential detection of virus isolates using RT-PCR     

Eiko Nakazono-Nagaoka  Chikako Sato  Yoshitaka Kosaka  Tomohide Natsuaki 《Journal of General Plant Pathology》2004,70(6):359-362

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