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Takayasu Moroki Saori Matsuo Hirofumi Hatakeyama Seigo Hayashi Izumi Matsumoto Satoshi Suzuki Takashi Kotera Kazuyoshi Kumagai Kiyokazu Ozaki 《Journal of toxicologic pathology》2021,34(2):161
With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017. 相似文献
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Takayasu Moroki Yutaka Yoshikawa Katsuhiko Yoshizawa Airo Tsubura Hiroyuki Yasui 《Journal of toxicologic pathology》2013,26(3):329-333
Vanadium has potential for use in diabetes therapy. Many investigators have reported toxic effects of inorganic vanadium salts; however, there are few reports on toxic effects of oxovanadium(VO2+) complexes. Therefore, we studied VO2+ toxicity by examining histological changes and measuring the vanadium concentration in the testis after repeated oral administration of bis(1-oxy-2-pyridine-thiolato)oxovanadium(VO2+) (VO(opt)2) for 2 or 4 weeks in KK-Ay mice. Severe mineralization and degeneration/necrosis of the seminiferous tubules were detected after either 2 or 4 weeks of administration. Vacuolar changes in Sertoli cells and the seminiferous epithelia, and hyperplasia of Leydig cells were observed in the testes of some animals. Vanadium concentrations in the mineralized testis were much higher than those in the testis of untreated KK-Ay mice. These results represent the first report of the possibility for seminiferous tubules mineralization induced by VO(opt)2 administration. Therefore, our research provides important information about the potentially toxic effects of VO2+ complexes. 相似文献
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Ukiya M Akihisa T Tokuda H Koike K Takayasu J Okuda H Kimura Y Nikaido T Nishino H 《Journal of agricultural and food chemistry》2003,51(10):2949-2957
Eight fatty acid esters of triterpene alcohols (1-8), four free triterpene alcohols (9, 12, 17, and 18), four diterpene acids (19-22), two tocopherol-related compounds (23 and 24), four estolides (25-28), three syn-alkane-4,6-diols (29-31), one 1,3-dioxoalkanoic acid (32), and one aliphatic ketone (33), along with the mixture of free fatty acids, were isolated from the diethyl ether extract of the pollen grains of sunflower (Helianthus annuus). Among these compounds, 14 (2-8, 12, 23, 25-28, and 33) were new naturally occurring compounds, and their structures were determined on the basis of spectroscopic methods. Twenty-four terpenoids and lipids (1-4, 6-9, 12, and 19-33) and six free triterpene triols (10, 11, and 13-16), derived from their fatty acid esters (2, 3, and 5-8) by alkaline hydrolysis, were evaluated with respect to their inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) induced by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in Raji cells, which is known to be a primary screening test for antitumor promoters. Among the 30 compounds tested, 21 compounds possessing a di- or a polycyclic ring system in the molecule (1-4, 6-16, and 19-24) showed potent inhibitory effects on EBV-EA induction (91-100% inhibition at 1 x 10(3) mol ratio/TPA). 相似文献
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Niwako Ogata Teppei Kanda Mizuki Kawahata Takayasu Ichikawa Yuki Matsumoto Waka Morimitsu Yukiko Nishino Takamasa Itoi Kayo Furumoto 《Veterinary anaesthesia and analgesia》2017,44(5):1091-1100
Objective
To determine the effects of brimonidine tartrate ophthalmic solution on sedation, heart rate (HR), respiratory frequency (fR), rectal temperature (RT) and noninvasive mean arterial pressure (MAP) in healthy cats.Study design
Randomized, blinded crossover study, with 1 week washout between treatments.Animals
Six healthy purpose-bred cats.Methods
Brimonidine tartrate ophthalmic solution 0.1% (one or two drops; 58.6 ± 3.3 μg per drop) or a control solution (artificial tear solution) was administered to six healthy cats. Behavioural observations and measurements of HR, fR, RT and MAP were recorded before and at 30, 60, 90, 120, 180, 240, 300 and 360 minutes after topical administration. Behavioural scores were analysed using Friedman’s test for repeated measures to evaluate the time effect in each treatment and treatment effect at each time point. Physiological variables (HR, fR, RT and MAP) were analysed using two-way analysis of variance for repeated measures to evaluate the time and treatment effects. The level of significance was set at p < 0.05.Results
Dose-dependent behavioural and physiological responses were noted. A dose of two drops of brimonidine resulted in sedation in the cats and decreased HR and MAP. Significant sedative effects occurred between 30 and 120 minutes and for physiological responses up to 360 minutes. The most frequent adverse reaction was vomiting, occurring within 40 minutes in all six cats administered two drops and five of the six cats administered one drop of brimonidine.Conclusions and clinical relevance
The results demonstrated that ocular administration of brimonidine 0.1% ophthalmic solution induced sedation in cats and some cardiovascular effects usually associated with α2-adrenoceptor agonists. Further studies should be performed to determine clinical applications for this agent in cats. 相似文献6.
To improve the welfare of caged laying hens, a pecking device made of stones was introduced on the cage floor. Twenty‐four White Leghorn hens aged 15 months were divided into four groups: single‐housed hens with device, single‐housed control hens, pair‐housed hens with device and pair‐housed control hens. Hens housed with the device pecked at various pecking objects less often than control hens. Agonistic behavior was also lower in hens with the device than in hens without the device, implied a possibility of improvement in quality of pecking stimuli with the device. Not only time spent pecking, but also quality of pecking might be important to fill their need for stimulation. Both single‐ and pair‐housed hens more often pecked at the device in the evening. Response to various pecking objects also showed that pecking behaviors were most frequently expressed in the evening. Increased foraging at dusk is a well‐known habit; therefore, the increase in pecking behavior in the evening might reflect the hens' general circadian rhythm. These results indicate that the device made of stones could promote some instinctive behavior. Enhancement of behavioral repertories and reduced agonistic behavior with the pecking device might improve the welfare of caged laying hens. 相似文献
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Takayasu Moroki Tomo Sasaki Katsuhiko Yoshizawa Takaaki Doi 《Journal of toxicologic pathology》2015,28(3):171-176
Pituicytoma is an extremely rare neoplasm derived from pituicytes, which are glial cells
in the posterior lobe of the pituitary gland. A malignant pituicytoma was found in the
intracranial cavity of a 55-week-old male Sprague-Dawley rat. Macroscopically, the tumor
was located on the sphenoid bone and involved the pituitary gland. The tumor was composed
of sheets of fusiform cells with spindle- or pleomorphic-shaped nuclei and abundant
eosinophilic cytoplasms. The cells were arranged in a whirling or irregular growth
pattern. Some tumor cells were bizarre multinucleated giant cells with cytoplasmic
eosinophilic hyaline droplets. Many tumor cells were strongly positive for vimentin and
glial fibrillary acidic protein, and some cells were positive for ED-1 and S-100. These
findings closely resembled those of a giant cell glioblastoma derived from the pituitary
gland, suggesting anaplastic pituicytoma. From our review of the literature, we believe
this is the first report of a spontaneous malignant pituicytoma in a rodent. 相似文献
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Iino M Goto K Kakegawa W Okado H Sudo M Ishiuchi S Miwa A Takayasu Y Saito I Tsuzuki K Ozawa S 《Science (New York, N.Y.)》2001,292(5518):926-929
Glial cells express a variety of neurotransmitter receptors. Notably, Bergmann glial cells in the cerebellum have Ca2+-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) assembled without the GluR2 subunit. To elucidate the role of these Ca2+-permeable AMPARs, we converted them into Ca2+-impermeable receptors by adenoviral-mediated delivery of the GluR2 gene. This conversion retracted the glial processes ensheathing synapses on Purkinje cell dendritic spines and retarded the removal of synaptically released glutamate. Furthermore, it caused multiple innervation of Purkinje cells by the climbing fibers. Thus, the glial Ca2+-permeable AMPARs are indispensable for proper structural and functional relations between Bergmann glia and glutamatergic synapses. 相似文献
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